Human mesenchymal stem cell-derived exosomes accelerate wound healing of mice eczema
Introduction The aim of our study was to investigate the therapeutic effect of Mesenchymal stem cell-derived exosomes (MSC-exs) on eczema mice model. Methods Eczema mice were established by 2, 4-two nitrochlorobenzene. Human umbilical cords cells and exosomes were harvested. In eczema mice model, th...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-04-01
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| Series: | Journal of Dermatological Treatment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09546634.2020.1820935 |
| _version_ | 1797683541634449408 |
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| author | Miao Wang Yang Zhao Qingyi Zhang |
| author_facet | Miao Wang Yang Zhao Qingyi Zhang |
| author_sort | Miao Wang |
| collection | DOAJ |
| description | Introduction The aim of our study was to investigate the therapeutic effect of Mesenchymal stem cell-derived exosomes (MSC-exs) on eczema mice model. Methods Eczema mice were established by 2, 4-two nitrochlorobenzene. Human umbilical cords cells and exosomes were harvested. In eczema mice model, the effect of MSC-ex on eczema was evaluated by severity score, atopic dermatitis score and histopathological analysis of dermis. MTT tests were performed to assess PBMC proliferation. Treg was identified by flow cytometry. The angiogenesis was analyzed by endothelial cell tube formation assay. Results Compared with PBS, the wound closure of animals treated with MSC-exs was faster. After MSC-exs treatment, there were more new epidermis and dermis, and less scar formation of the lesion. There were significant differences in the integral score of skin injury and the number of lymphocyte infiltration in the skin between the treatment group and the PBS group (p < .01). MSC-exs significantly inhibit Peripheral blood mononuclear cell proliferation, promote the transformation of Treg and the formation of endothelial tube. Conclusion MSC-ex accelerated wounds healing in mice eczema model by inhibiting inflammatory cell infiltration and promoting vascular formation. |
| first_indexed | 2024-03-12T00:16:31Z |
| format | Article |
| id | doaj.art-fa52711f518247e984a1178686248f58 |
| institution | Directory Open Access Journal |
| issn | 0954-6634 1471-1753 |
| language | English |
| last_indexed | 2024-03-12T00:16:31Z |
| publishDate | 2022-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Dermatological Treatment |
| spelling | doaj.art-fa52711f518247e984a1178686248f582023-09-15T14:28:49ZengTaylor & Francis GroupJournal of Dermatological Treatment0954-66341471-17532022-04-013331401140510.1080/09546634.2020.18209351820935Human mesenchymal stem cell-derived exosomes accelerate wound healing of mice eczemaMiao Wang0Yang Zhao1Qingyi Zhang2Department of Pathology, Beijing Friend Hospital, The Second Clinical Medical College of Capital Medical UniversityMilitary Medical Science Academy, The PLA Institute of Radiation MedicineDepartment of Hematology of Air Force, PLA General HospitalIntroduction The aim of our study was to investigate the therapeutic effect of Mesenchymal stem cell-derived exosomes (MSC-exs) on eczema mice model. Methods Eczema mice were established by 2, 4-two nitrochlorobenzene. Human umbilical cords cells and exosomes were harvested. In eczema mice model, the effect of MSC-ex on eczema was evaluated by severity score, atopic dermatitis score and histopathological analysis of dermis. MTT tests were performed to assess PBMC proliferation. Treg was identified by flow cytometry. The angiogenesis was analyzed by endothelial cell tube formation assay. Results Compared with PBS, the wound closure of animals treated with MSC-exs was faster. After MSC-exs treatment, there were more new epidermis and dermis, and less scar formation of the lesion. There were significant differences in the integral score of skin injury and the number of lymphocyte infiltration in the skin between the treatment group and the PBS group (p < .01). MSC-exs significantly inhibit Peripheral blood mononuclear cell proliferation, promote the transformation of Treg and the formation of endothelial tube. Conclusion MSC-ex accelerated wounds healing in mice eczema model by inhibiting inflammatory cell infiltration and promoting vascular formation.http://dx.doi.org/10.1080/09546634.2020.1820935eczemamesenchymal stem cellsexosome |
| spellingShingle | Miao Wang Yang Zhao Qingyi Zhang Human mesenchymal stem cell-derived exosomes accelerate wound healing of mice eczema Journal of Dermatological Treatment eczema mesenchymal stem cells exosome |
| title | Human mesenchymal stem cell-derived exosomes accelerate wound healing of mice eczema |
| title_full | Human mesenchymal stem cell-derived exosomes accelerate wound healing of mice eczema |
| title_fullStr | Human mesenchymal stem cell-derived exosomes accelerate wound healing of mice eczema |
| title_full_unstemmed | Human mesenchymal stem cell-derived exosomes accelerate wound healing of mice eczema |
| title_short | Human mesenchymal stem cell-derived exosomes accelerate wound healing of mice eczema |
| title_sort | human mesenchymal stem cell derived exosomes accelerate wound healing of mice eczema |
| topic | eczema mesenchymal stem cells exosome |
| url | http://dx.doi.org/10.1080/09546634.2020.1820935 |
| work_keys_str_mv | AT miaowang humanmesenchymalstemcellderivedexosomesacceleratewoundhealingofmiceeczema AT yangzhao humanmesenchymalstemcellderivedexosomesacceleratewoundhealingofmiceeczema AT qingyizhang humanmesenchymalstemcellderivedexosomesacceleratewoundhealingofmiceeczema |