High Manganese Tolerance and Biooxidation Ability of Serratia marcescens Isolated from Manganese Mine Water in Minas Gerais, Brazil
Manganese is an important metal for the maintenance of several biological functions, but it can be toxic in high concentrations. One of the main forms of human exposure to metals, such as manganese (Mn), is the consumption of solar salt contaminated. Mn-tolerant bacteria could be used to decrease th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01946/full |
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| author | Natália R. Barboza Mônica M. C. A. Morais Pollyana S. Queiroz Soraya S. Amorim Renata Guerra-Sá Versiane A. Leão |
| author_facet | Natália R. Barboza Mônica M. C. A. Morais Pollyana S. Queiroz Soraya S. Amorim Renata Guerra-Sá Versiane A. Leão |
| author_sort | Natália R. Barboza |
| collection | DOAJ |
| description | Manganese is an important metal for the maintenance of several biological functions, but it can be toxic in high concentrations. One of the main forms of human exposure to metals, such as manganese (Mn), is the consumption of solar salt contaminated. Mn-tolerant bacteria could be used to decrease the concentration of this metal from contaminated sites through safer environmental-friendly alternative technology in the future. Therefore, this study was undertaken to isolate and identify Mn resistant bacteria from water samples collected from a Mn mine in the Iron Quadrangle region (Minas Gerais, Brazil). Two bacterial isolates were identified as Serratia marcescens based on morphological, biochemical, 16S rDNA gene sequencing and phylogeny analysis. Maximum resistance of the selected isolates against increasing concentrations of Mn(II), up to 1200 mg L-1 was determined in solid media. A batch assay was developed to analyze and quantify the Mn removal capacities of the isolates. Biological Mn removal capacities of over 55% were detected for both isolates. Whereas that mechanism like biosorption, precipitation and oxidation could be explaining the Mn removal, we seek to give an insight into some of the molecular mechanisms adopted by S. marcescens isolates. For this purpose, the following approaches were adopted: leucoberbelin blue I assay, Mn(II) oxidation by cell-free filtrate and electron microscopy and energy-dispersive X-ray spectroscopy analyses. Overall, these results indicate that S. marcescens promotes Mn removal in an indirect mechanism by the formation of Mn oxides precipitates around the cells, which should be further explored for potential biotechnological applications for water recycling both in hydrometallurgical and mineral processing operations. |
| first_indexed | 2024-04-13T10:55:05Z |
| format | Article |
| id | doaj.art-fa5480f286b74af1abb7dfea7dd6c6e6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T10:55:05Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-fa5480f286b74af1abb7dfea7dd6c6e62022-12-22T02:49:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-10-01810.3389/fmicb.2017.01946287492High Manganese Tolerance and Biooxidation Ability of Serratia marcescens Isolated from Manganese Mine Water in Minas Gerais, BrazilNatália R. Barboza0Mônica M. C. A. Morais1Pollyana S. Queiroz2Soraya S. Amorim3Renata Guerra-Sá4Versiane A. Leão5Laboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológica (NUPEB), Universidade Federal de Ouro Preto, Ouro Preto, BrazilUnileste, Ipatinga, BrazilLaboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológica (NUPEB), Universidade Federal de Ouro Preto, Ouro Preto, BrazilLaboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológica (NUPEB), Universidade Federal de Ouro Preto, Ouro Preto, BrazilLaboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológica (NUPEB), Universidade Federal de Ouro Preto, Ouro Preto, BrazilLaboratório de Bio&Hidrometalurgia, Departamento de Engenharia Metalúrgica e de Materiais, Escola de Minas, Universidade Federal de Ouro Preto, Ouro Preto, BrazilManganese is an important metal for the maintenance of several biological functions, but it can be toxic in high concentrations. One of the main forms of human exposure to metals, such as manganese (Mn), is the consumption of solar salt contaminated. Mn-tolerant bacteria could be used to decrease the concentration of this metal from contaminated sites through safer environmental-friendly alternative technology in the future. Therefore, this study was undertaken to isolate and identify Mn resistant bacteria from water samples collected from a Mn mine in the Iron Quadrangle region (Minas Gerais, Brazil). Two bacterial isolates were identified as Serratia marcescens based on morphological, biochemical, 16S rDNA gene sequencing and phylogeny analysis. Maximum resistance of the selected isolates against increasing concentrations of Mn(II), up to 1200 mg L-1 was determined in solid media. A batch assay was developed to analyze and quantify the Mn removal capacities of the isolates. Biological Mn removal capacities of over 55% were detected for both isolates. Whereas that mechanism like biosorption, precipitation and oxidation could be explaining the Mn removal, we seek to give an insight into some of the molecular mechanisms adopted by S. marcescens isolates. For this purpose, the following approaches were adopted: leucoberbelin blue I assay, Mn(II) oxidation by cell-free filtrate and electron microscopy and energy-dispersive X-ray spectroscopy analyses. Overall, these results indicate that S. marcescens promotes Mn removal in an indirect mechanism by the formation of Mn oxides precipitates around the cells, which should be further explored for potential biotechnological applications for water recycling both in hydrometallurgical and mineral processing operations.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01946/fullmanganesebioremediationSerratia marcescensmanganese oxidationbiooxidation |
| spellingShingle | Natália R. Barboza Mônica M. C. A. Morais Pollyana S. Queiroz Soraya S. Amorim Renata Guerra-Sá Versiane A. Leão High Manganese Tolerance and Biooxidation Ability of Serratia marcescens Isolated from Manganese Mine Water in Minas Gerais, Brazil Frontiers in Microbiology manganese bioremediation Serratia marcescens manganese oxidation biooxidation |
| title | High Manganese Tolerance and Biooxidation Ability of Serratia marcescens Isolated from Manganese Mine Water in Minas Gerais, Brazil |
| title_full | High Manganese Tolerance and Biooxidation Ability of Serratia marcescens Isolated from Manganese Mine Water in Minas Gerais, Brazil |
| title_fullStr | High Manganese Tolerance and Biooxidation Ability of Serratia marcescens Isolated from Manganese Mine Water in Minas Gerais, Brazil |
| title_full_unstemmed | High Manganese Tolerance and Biooxidation Ability of Serratia marcescens Isolated from Manganese Mine Water in Minas Gerais, Brazil |
| title_short | High Manganese Tolerance and Biooxidation Ability of Serratia marcescens Isolated from Manganese Mine Water in Minas Gerais, Brazil |
| title_sort | high manganese tolerance and biooxidation ability of serratia marcescens isolated from manganese mine water in minas gerais brazil |
| topic | manganese bioremediation Serratia marcescens manganese oxidation biooxidation |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01946/full |
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