Repellency and toxicity of the crude ethanolic extract of Limoniastrum guyonianum against Tribolium castaneum. Acheuk, F., Belaid, M., Lakhdari, W., Abdellaoui, K., Dehliz, A., and Mokrane, K. (Algeria/Tunisia)
In nature, the interaction between plants and insects has led to the production of a set of secondary compounds. Many plant secondary metabolites have significant insecticidal activity. The aim of this study is to evaluate the repellent and insecticidal effect of the crude ethanolic extract of Limon...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Institution of the Agricultural Research and Higher Education
2017-03-01
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| Series: | Tunisian Journal of Plant Protection |
| Subjects: | |
| Online Access: | http://www.tjpp.tn/SiteWeb/TJPPsiSite/CurrentIssue/6Acheuk.pdf
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| Summary: | In nature, the interaction between plants and insects has led to the production of a set of secondary
compounds. Many plant secondary metabolites have significant insecticidal activity. The aim of this
study is to evaluate the repellent and insecticidal effect of the crude ethanolic extract of Limoniastrum
guyonianum against adults of the red flour beetle Tribolium castaneum. For the insecticidal activity,
five doses (100, 200, 400, 600, and 800 µg/insect) were tested and were topically applied onto insect
thorax. An area preference method was adopted to assess the repellent activity. A phytochemical study
and measurement of two enzymatic biomarkers: acetylcholinesterase (AChE) and gluthatione Stransferase
(GST) were made to understand the mechanisms of toxic action of the tested extract.
Phytochemical study showed the presence of various groups of natural products. The plant is rich in
flavonoids, tannins, alkaloids, and glycosides. Low amount of saponins was noted. The study also
showed that this plant does not contain iridoids. For repellent activity, the results showed that the
highest dose (800 µg/insect) exhibited obvious repellent effect against T. castaneum. The repellency
percentage was 90.14 ± 2.5% after 4 h of exposure. The crude extract was found to be toxic to T.
castaneum and the corresponding LD50value was 218.3 μg/insect. Moreover, the extract inhibits the
activity of the acetylcholinesterase (IC50: 205.7 µg/insect). |
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| ISSN: | 1737-5436 2490-4368 |