Identification of cholesterol gallstone with two-dimensional gel electrophoresis and mass spectrometry
Background and rationale. We aimed to provide novel information to better understand the molecular mechanisms underlying gallstones formation and explore the potential protein markers for gallstones progression. The gallbladder tissues were collected from 20 patients with cholesterol gallstone and 1...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-03-01
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| Series: | Annals of Hepatology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268119307884 |
| _version_ | 1830200278128787456 |
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| author | Changlin Qian Zhiyong Shen Jie Zhang Fu Ji Hua Liu |
| author_facet | Changlin Qian Zhiyong Shen Jie Zhang Fu Ji Hua Liu |
| author_sort | Changlin Qian |
| collection | DOAJ |
| description | Background and rationale. We aimed to provide novel information to better understand the molecular mechanisms underlying gallstones formation and explore the potential protein markers for gallstones progression. The gallbladder tissues were collected from 20 patients with cholesterol gallstone and 10 liver transplant donors from November 2010 to April 2011. The proteomics were compared between gallstone patients and controls by two-dimensional gel electrophoresis (2-DE). The differentially expressed proteins were identified and validated by western blotting and real-time PCR.Results. Total 19 protein spots were found to be different between two groups and 11 proteins were identified, among which 4 ones (such as Peroxiredoxin 3/Prdx3) were down-regulated and 7 (such as Tropomyosin 4/TPM4, Transgelin/SM22, Transthyretin/TTR) were up-regulated in gallstone group. Results of western blotting and RT-PCR were consistent with the 2-DE results. Conclusion. The differentially expressed proteins of TTR, TPM4, SM22 and Prdx3 may play key roles in gallstone formation and may be markers for gallstone progression. |
| first_indexed | 2024-12-18T02:30:04Z |
| format | Article |
| id | doaj.art-fa58b6eaf5cd443697689d75aa7d4b51 |
| institution | Directory Open Access Journal |
| issn | 1665-2681 |
| language | English |
| last_indexed | 2024-12-18T02:30:04Z |
| publishDate | 2015-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj.art-fa58b6eaf5cd443697689d75aa7d4b512022-12-21T21:23:55ZengElsevierAnnals of Hepatology1665-26812015-03-01142251258Identification of cholesterol gallstone with two-dimensional gel electrophoresis and mass spectrometryChanglin Qian0Zhiyong Shen1Jie Zhang2Fu Ji3Hua Liu4Department of General Surgery, South Campus, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 201112. ChinaDepartment of General Surgery, South Campus, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 201112. ChinaDepartment of General Surgery, South Campus, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 201112. ChinaDepartment of General Surgery, South Campus, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 201112. ChinaDepartment of General Surgery, South Campus, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 201112. China; Correspondence and reprint request:Background and rationale. We aimed to provide novel information to better understand the molecular mechanisms underlying gallstones formation and explore the potential protein markers for gallstones progression. The gallbladder tissues were collected from 20 patients with cholesterol gallstone and 10 liver transplant donors from November 2010 to April 2011. The proteomics were compared between gallstone patients and controls by two-dimensional gel electrophoresis (2-DE). The differentially expressed proteins were identified and validated by western blotting and real-time PCR.Results. Total 19 protein spots were found to be different between two groups and 11 proteins were identified, among which 4 ones (such as Peroxiredoxin 3/Prdx3) were down-regulated and 7 (such as Tropomyosin 4/TPM4, Transgelin/SM22, Transthyretin/TTR) were up-regulated in gallstone group. Results of western blotting and RT-PCR were consistent with the 2-DE results. Conclusion. The differentially expressed proteins of TTR, TPM4, SM22 and Prdx3 may play key roles in gallstone formation and may be markers for gallstone progression.http://www.sciencedirect.com/science/article/pii/S1665268119307884Molecular mechanismsProteomics |
| spellingShingle | Changlin Qian Zhiyong Shen Jie Zhang Fu Ji Hua Liu Identification of cholesterol gallstone with two-dimensional gel electrophoresis and mass spectrometry Annals of Hepatology Molecular mechanisms Proteomics |
| title | Identification of cholesterol gallstone with two-dimensional gel electrophoresis and mass spectrometry |
| title_full | Identification of cholesterol gallstone with two-dimensional gel electrophoresis and mass spectrometry |
| title_fullStr | Identification of cholesterol gallstone with two-dimensional gel electrophoresis and mass spectrometry |
| title_full_unstemmed | Identification of cholesterol gallstone with two-dimensional gel electrophoresis and mass spectrometry |
| title_short | Identification of cholesterol gallstone with two-dimensional gel electrophoresis and mass spectrometry |
| title_sort | identification of cholesterol gallstone with two dimensional gel electrophoresis and mass spectrometry |
| topic | Molecular mechanisms Proteomics |
| url | http://www.sciencedirect.com/science/article/pii/S1665268119307884 |
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