An Integrated Approach for the Efficient Extraction and Solubilization of Rice Microsomal Membrane Proteins for High-Throughput Proteomics

An Integrated Approach for the Efficient Extraction and Solubilization of Rice Microsomal Membrane Proteins for High-Throughput Proteomics

The preparation of microsomal membrane proteins (MPs) is critically important to microsomal proteomics. To date most research studies have utilized an ultracentrifugation-based approach for the isolation and solubilization of plant MPs. However, these approaches are labor-intensive, time-consuming,...

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Main Authors: Truong Van Nguyen, Ravi Gupta, Dicky Annas, Jinmi Yoon, Yu-Jin Kim, Gi Hyun Lee, Jeong Woo Jang, Kang Hyun Park, Randeep Rakwal, Ki-Hong Jung, Cheol Woo Min, Sun Tae Kim
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-09-01
Series:Frontiers in Plant Science
Subjects:
rice
label-free quantitative proteomics
microsomal membrane extraction
ultracentrifugation
AZO
sds
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.723369/full

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