In Vitro Antibacterial Activity of Selected Palestinian Medicinal Plants against <i>Chlamydia trachomatis</i>
<i>Chlamydia</i> spp. are intracellular pathogens of humans and animals that cause a wide range of diseases such as blinding trachoma and sexually transmitted infections. According to the World Health Organization (WHO), there are more than 127 million new infections each year worldwide....
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MDPI AG
2021-08-01
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| Series: | Microbiology Research |
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| Online Access: | https://www.mdpi.com/2036-7481/12/3/47 |
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| author | Omar Hamarsheh Ahmad Amro Munir A. Al-Zeer |
| author_facet | Omar Hamarsheh Ahmad Amro Munir A. Al-Zeer |
| author_sort | Omar Hamarsheh |
| collection | DOAJ |
| description | <i>Chlamydia</i> spp. are intracellular pathogens of humans and animals that cause a wide range of diseases such as blinding trachoma and sexually transmitted infections. According to the World Health Organization (WHO), there are more than 127 million new infections each year worldwide. Chlamydial urogenital infections can cause cervicitis, urethritis, pelvic inflammatory disease and infertility. From within an intracellular niche, termed an inclusion, the <i>Chlamydiae</i> complete their life cycle shielded from host defenses. The host cell defense response used to eliminate the pathogen must subvert this protective shield and is thought to involve the gamma interferon-inducible family of immunity related GTPase proteins and nitric oxide. Typically, azithromycin and doxycycline are the first line drugs for the treatment of chlamydial infections. Although <i>C. trachomatis</i> is sensitive to these antibiotics in vitro, currently, there is increasing bacterial resistance to antibiotics including multidrug-resistant <i>C. trachomatis</i>, which have been described in many instances. Therefore, alternative drug candidates against <i>Chlamydia</i> should be assessed in vitro. In this study, we tested and quantified the activity of plant extracts against <i>Chlamydia</i>-infected HeLa cells with <i>C. trachomatis</i> inclusions. The in vitro results show that post-treatment with <i>Artemisia inculta</i> Delile extract significantly inhibits <i>Chlamydia</i> infection compared to DMSO-treated samples. In conclusion, plant extracts may contain active ingredients with antichlamydial activity potential and can be used as alternative drug candidates for treatment of <i>Chlamydia</i> infection which has significant socio-economic and medical impact. |
| first_indexed | 2024-03-10T07:26:38Z |
| format | Article |
| id | doaj.art-fa5e1236f5584b5dbe3346c5ca62db39 |
| institution | Directory Open Access Journal |
| issn | 2036-7481 |
| language | English |
| last_indexed | 2024-03-10T07:26:38Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj.art-fa5e1236f5584b5dbe3346c5ca62db392023-11-22T14:14:59ZengMDPI AGMicrobiology Research2036-74812021-08-0112365666210.3390/microbiolres12030047In Vitro Antibacterial Activity of Selected Palestinian Medicinal Plants against <i>Chlamydia trachomatis</i>Omar Hamarsheh0Ahmad Amro1Munir A. Al-Zeer2Department of Life Sciences, College of Science and Technology, Al-Quds University, Jerusalem, PalestineFaculty of Pharmacy, Al-Quds University, Jerusalem, PalestineDepartment of Applied Biochemistry, Institute of Biotechnology, 4/3-2, Technical University of Berlin, Gustav-Meyer-Allee 25, 13355 Berlin, Germany<i>Chlamydia</i> spp. are intracellular pathogens of humans and animals that cause a wide range of diseases such as blinding trachoma and sexually transmitted infections. According to the World Health Organization (WHO), there are more than 127 million new infections each year worldwide. Chlamydial urogenital infections can cause cervicitis, urethritis, pelvic inflammatory disease and infertility. From within an intracellular niche, termed an inclusion, the <i>Chlamydiae</i> complete their life cycle shielded from host defenses. The host cell defense response used to eliminate the pathogen must subvert this protective shield and is thought to involve the gamma interferon-inducible family of immunity related GTPase proteins and nitric oxide. Typically, azithromycin and doxycycline are the first line drugs for the treatment of chlamydial infections. Although <i>C. trachomatis</i> is sensitive to these antibiotics in vitro, currently, there is increasing bacterial resistance to antibiotics including multidrug-resistant <i>C. trachomatis</i>, which have been described in many instances. Therefore, alternative drug candidates against <i>Chlamydia</i> should be assessed in vitro. In this study, we tested and quantified the activity of plant extracts against <i>Chlamydia</i>-infected HeLa cells with <i>C. trachomatis</i> inclusions. The in vitro results show that post-treatment with <i>Artemisia inculta</i> Delile extract significantly inhibits <i>Chlamydia</i> infection compared to DMSO-treated samples. In conclusion, plant extracts may contain active ingredients with antichlamydial activity potential and can be used as alternative drug candidates for treatment of <i>Chlamydia</i> infection which has significant socio-economic and medical impact.https://www.mdpi.com/2036-7481/12/3/47antibacterial activity<i>Chlamydia trachomatis</i>medicinal plantsPalestine |
| spellingShingle | Omar Hamarsheh Ahmad Amro Munir A. Al-Zeer In Vitro Antibacterial Activity of Selected Palestinian Medicinal Plants against <i>Chlamydia trachomatis</i> Microbiology Research antibacterial activity <i>Chlamydia trachomatis</i> medicinal plants Palestine |
| title | In Vitro Antibacterial Activity of Selected Palestinian Medicinal Plants against <i>Chlamydia trachomatis</i> |
| title_full | In Vitro Antibacterial Activity of Selected Palestinian Medicinal Plants against <i>Chlamydia trachomatis</i> |
| title_fullStr | In Vitro Antibacterial Activity of Selected Palestinian Medicinal Plants against <i>Chlamydia trachomatis</i> |
| title_full_unstemmed | In Vitro Antibacterial Activity of Selected Palestinian Medicinal Plants against <i>Chlamydia trachomatis</i> |
| title_short | In Vitro Antibacterial Activity of Selected Palestinian Medicinal Plants against <i>Chlamydia trachomatis</i> |
| title_sort | in vitro antibacterial activity of selected palestinian medicinal plants against i chlamydia trachomatis i |
| topic | antibacterial activity <i>Chlamydia trachomatis</i> medicinal plants Palestine |
| url | https://www.mdpi.com/2036-7481/12/3/47 |
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