A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin
Boonnada Pamornpathomkul,1 Adisak Wongkajornsilp,2 Wanida Laiwattanapaisal,3 Theerasak Rojanarata,1 Praneet Opanasopit,1 Tanasait Ngawhirunpat1 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group, Silpakorn University, Nakhon Pathom,...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2017-01-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/a-combined-approach-of-hollow-microneedles-and-nanocarriers-for-skin-i-peer-reviewed-article-IJN |
| _version_ | 1818733856031768576 |
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| author | Pamornpathomkul B Wongkajornsilp A Laiwattanapaisal W Rojanarata T Opanasopit P Ngawhirunpat T |
| author_facet | Pamornpathomkul B Wongkajornsilp A Laiwattanapaisal W Rojanarata T Opanasopit P Ngawhirunpat T |
| author_sort | Pamornpathomkul B |
| collection | DOAJ |
| description | Boonnada Pamornpathomkul,1 Adisak Wongkajornsilp,2 Wanida Laiwattanapaisal,3 Theerasak Rojanarata,1 Praneet Opanasopit,1 Tanasait Ngawhirunpat1 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group, Silpakorn University, Nakhon Pathom, 2Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 3Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand Abstract: The aim of this study was to investigate the use of different types of microneedles (MNs) and nanocarriers for in vitro skin permeation and in vivo immunization of plasmid DNA encoding ovalbumin (pOVA). In vitro skin permeation studies indicated that hollow MNs had a superior enhancing effect on skin permeation compared with solid MN patches, electroporation (EP) patches, the combination of MN and EP patches, and untreated skin. Upon using hollow MNs combined with nanocarriers for pOVA delivery, the skin permeation was higher than for the delivery of naked pOVA, as evidenced by the increased amount of pOVA in Franz diffusion cells and immunoglobulin G (IgG) antibody responses. When the hollow MNs were used for the delivery of nanocarrier:pOVA complexes into the skin of mice, they induced a stronger IgG immune response than conventional subcutaneous (SC) injections. In addition, immunization of mice with the hollow MNs did not induce signs of skin infection or pinpoint bleeding. Accordingly, the hollow MNs combined with a nanocarrier delivery system is a promising approach for delivering pOVA complexes to the skin for promoting successful immunization. Keywords: hollow microneedle, solid microneedle, electroporation, plasmid DNA encoding ovalbumin, skin immunization, nanocarrier |
| first_indexed | 2024-12-17T23:56:06Z |
| format | Article |
| id | doaj.art-fa611eea63a447e1b5398974630a22d2 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-17T23:56:06Z |
| publishDate | 2017-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-fa611eea63a447e1b5398974630a22d22022-12-21T21:28:03ZengDove Medical PressInternational Journal of Nanomedicine1178-20132017-01-01Volume 1288589831049A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbuminPamornpathomkul BWongkajornsilp ALaiwattanapaisal WRojanarata TOpanasopit PNgawhirunpat TBoonnada Pamornpathomkul,1 Adisak Wongkajornsilp,2 Wanida Laiwattanapaisal,3 Theerasak Rojanarata,1 Praneet Opanasopit,1 Tanasait Ngawhirunpat1 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group, Silpakorn University, Nakhon Pathom, 2Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 3Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand Abstract: The aim of this study was to investigate the use of different types of microneedles (MNs) and nanocarriers for in vitro skin permeation and in vivo immunization of plasmid DNA encoding ovalbumin (pOVA). In vitro skin permeation studies indicated that hollow MNs had a superior enhancing effect on skin permeation compared with solid MN patches, electroporation (EP) patches, the combination of MN and EP patches, and untreated skin. Upon using hollow MNs combined with nanocarriers for pOVA delivery, the skin permeation was higher than for the delivery of naked pOVA, as evidenced by the increased amount of pOVA in Franz diffusion cells and immunoglobulin G (IgG) antibody responses. When the hollow MNs were used for the delivery of nanocarrier:pOVA complexes into the skin of mice, they induced a stronger IgG immune response than conventional subcutaneous (SC) injections. In addition, immunization of mice with the hollow MNs did not induce signs of skin infection or pinpoint bleeding. Accordingly, the hollow MNs combined with a nanocarrier delivery system is a promising approach for delivering pOVA complexes to the skin for promoting successful immunization. Keywords: hollow microneedle, solid microneedle, electroporation, plasmid DNA encoding ovalbumin, skin immunization, nanocarrierhttps://www.dovepress.com/a-combined-approach-of-hollow-microneedles-and-nanocarriers-for-skin-i-peer-reviewed-article-IJNHollow microneedleSolid microneedleElectroporationPlasmid OvalbuminSkin immunizationNanocarrier |
| spellingShingle | Pamornpathomkul B Wongkajornsilp A Laiwattanapaisal W Rojanarata T Opanasopit P Ngawhirunpat T A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin International Journal of Nanomedicine Hollow microneedle Solid microneedle Electroporation Plasmid Ovalbumin Skin immunization Nanocarrier |
| title | A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin |
| title_full | A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin |
| title_fullStr | A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin |
| title_full_unstemmed | A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin |
| title_short | A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin |
| title_sort | combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid dna encoding ovalbumin |
| topic | Hollow microneedle Solid microneedle Electroporation Plasmid Ovalbumin Skin immunization Nanocarrier |
| url | https://www.dovepress.com/a-combined-approach-of-hollow-microneedles-and-nanocarriers-for-skin-i-peer-reviewed-article-IJN |
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