Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics
The development of high-throughput sequencing (HTS) technologies and metagenomics protocols deeply impacted the discovery of viral diversity. Moreover, the characterization of novel viruses in the Neotropical primates (NP) is central for the comprehension of viral evolution dynamics in those hosts,...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1002963/full |
| _version_ | 1798002195768016896 |
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| author | Matheus Augusto Calvano Cosentino Mirela D’arc Filipe Romero Rebello Moreira Filipe Romero Rebello Moreira Liliane Tavares de Faria Cavalcante Ricardo Mouta Amanda Coimbra Francine Bittencourt Schiffler Thamiris dos Santos Miranda Gabriel Medeiros Cecilia A. Dias Antonizete R. Souza Maria Clotilde Henriques Tavares Amilcar Tanuri Marcelo Alves Soares Marcelo Alves Soares André Felipe Andrade dos Santos |
| author_facet | Matheus Augusto Calvano Cosentino Mirela D’arc Filipe Romero Rebello Moreira Filipe Romero Rebello Moreira Liliane Tavares de Faria Cavalcante Ricardo Mouta Amanda Coimbra Francine Bittencourt Schiffler Thamiris dos Santos Miranda Gabriel Medeiros Cecilia A. Dias Antonizete R. Souza Maria Clotilde Henriques Tavares Amilcar Tanuri Marcelo Alves Soares Marcelo Alves Soares André Felipe Andrade dos Santos |
| author_sort | Matheus Augusto Calvano Cosentino |
| collection | DOAJ |
| description | The development of high-throughput sequencing (HTS) technologies and metagenomics protocols deeply impacted the discovery of viral diversity. Moreover, the characterization of novel viruses in the Neotropical primates (NP) is central for the comprehension of viral evolution dynamics in those hosts, due to their evolutionary proximity to Old World primates, including humans. In the present work, novel anelloviruses were detected and characterized through HTS protocols in the NP Callithrix penicillata, the common black-tufted marmoset. De novo assembly of generated sequences was carried out, and a total of 15 contigs were identified with complete Anelloviridae ORF1 gene, two of them including a flanking GC-rich region, confirming the presence of two whole novel genomes of ~3 kb. The identified viruses were monophyletic within the Epsilontorquevirus genus, a lineage harboring previously reported anelloviruses infecting hosts from the Cebidae family. The genetic divergence found in the new viruses characterized two novel species, named Epsilontorquevirus callithrichensis I and II. The phylogenetic pattern inferred for the Epsilontorquevirus genus was consistent with the topology of their host species tree, echoing a virus-host diversification model observed in other viral groups. This study expands the host span of Anelloviridae and provides insights into their diversification dynamics, highlighting the importance of sampling animal viral genomes to obtain a clearer depiction of their long-term evolutionary processes. |
| first_indexed | 2024-04-11T11:48:16Z |
| format | Article |
| id | doaj.art-fa61a092a46d408faae74fc64bc6f694 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T11:48:16Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-fa61a092a46d408faae74fc64bc6f6942022-12-22T04:25:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.10029631002963Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamicsMatheus Augusto Calvano Cosentino0Mirela D’arc1Filipe Romero Rebello Moreira2Filipe Romero Rebello Moreira3Liliane Tavares de Faria Cavalcante4Ricardo Mouta5Amanda Coimbra6Francine Bittencourt Schiffler7Thamiris dos Santos Miranda8Gabriel Medeiros9Cecilia A. Dias10Antonizete R. Souza11Maria Clotilde Henriques Tavares12Amilcar Tanuri13Marcelo Alves Soares14Marcelo Alves Soares15André Felipe Andrade dos Santos16Laboratório de Diversidade e Doenças Virais, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Diversidade e Doenças Virais, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Diversidade e Doenças Virais, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartment of Infectious Diseases Epidemiology, Imperial College London, London, United KingdomLaboratório de Diversidade e Doenças Virais, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Diversidade e Doenças Virais, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Diversidade e Doenças Virais, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Diversidade e Doenças Virais, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Diversidade e Doenças Virais, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Diversidade e Doenças Virais, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilCentro de Primatologia, Universidade de Brasília, Brasília, BrazilCentro de Primatologia, Universidade de Brasília, Brasília, BrazilCentro de Primatologia, Universidade de Brasília, Brasília, BrazilLaboratório de Virologia Molecular, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Diversidade e Doenças Virais, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilPrograma de Oncovirologia, Instituto Nacional de Câncer, Rio de Janeiro, BrazilLaboratório de Diversidade e Doenças Virais, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilThe development of high-throughput sequencing (HTS) technologies and metagenomics protocols deeply impacted the discovery of viral diversity. Moreover, the characterization of novel viruses in the Neotropical primates (NP) is central for the comprehension of viral evolution dynamics in those hosts, due to their evolutionary proximity to Old World primates, including humans. In the present work, novel anelloviruses were detected and characterized through HTS protocols in the NP Callithrix penicillata, the common black-tufted marmoset. De novo assembly of generated sequences was carried out, and a total of 15 contigs were identified with complete Anelloviridae ORF1 gene, two of them including a flanking GC-rich region, confirming the presence of two whole novel genomes of ~3 kb. The identified viruses were monophyletic within the Epsilontorquevirus genus, a lineage harboring previously reported anelloviruses infecting hosts from the Cebidae family. The genetic divergence found in the new viruses characterized two novel species, named Epsilontorquevirus callithrichensis I and II. The phylogenetic pattern inferred for the Epsilontorquevirus genus was consistent with the topology of their host species tree, echoing a virus-host diversification model observed in other viral groups. This study expands the host span of Anelloviridae and provides insights into their diversification dynamics, highlighting the importance of sampling animal viral genomes to obtain a clearer depiction of their long-term evolutionary processes.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1002963/fullmetagenomicmarmosetco-divergencessDNA virusesvirus evolution |
| spellingShingle | Matheus Augusto Calvano Cosentino Mirela D’arc Filipe Romero Rebello Moreira Filipe Romero Rebello Moreira Liliane Tavares de Faria Cavalcante Ricardo Mouta Amanda Coimbra Francine Bittencourt Schiffler Thamiris dos Santos Miranda Gabriel Medeiros Cecilia A. Dias Antonizete R. Souza Maria Clotilde Henriques Tavares Amilcar Tanuri Marcelo Alves Soares Marcelo Alves Soares André Felipe Andrade dos Santos Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics Frontiers in Microbiology metagenomic marmoset co-divergence ssDNA viruses virus evolution |
| title | Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics |
| title_full | Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics |
| title_fullStr | Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics |
| title_full_unstemmed | Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics |
| title_short | Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics |
| title_sort | discovery of two novel torque teno viruses in callithrix penicillata provides insights on anelloviridae diversification dynamics |
| topic | metagenomic marmoset co-divergence ssDNA viruses virus evolution |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1002963/full |
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