Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia
This study aims to investigate the morphological and biochemical characteristics of thermophilic bacteria isolated from thermophilic biotopes in the Al-Jouf region of Saudi Arabia for the first time. Additionally, the production of thermostable bacterial enzymes (proteases, amylases, cellulases, gel...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2024-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/characterization-and-identification-of-thermophilic-bacteria-isolated-from-different-sites-located-in-al-jouf-region-saudi-arabia/ |
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| author | Sherifa A. Saidan Raja Jarboui Salam S. Alsharari Mona S. Azab |
| author_facet | Sherifa A. Saidan Raja Jarboui Salam S. Alsharari Mona S. Azab |
| author_sort | Sherifa A. Saidan |
| collection | DOAJ |
| description | This study aims to investigate the morphological and biochemical characteristics of thermophilic bacteria isolated from thermophilic biotopes in the Al-Jouf region of Saudi Arabia for the first time. Additionally, the production of thermostable bacterial enzymes (proteases, amylases, cellulases, gelatinases, and lipases) was investigated. Six main bacterial species were identified via 16S rRNA sequencing and phylogenetic analysis. Twenty water and soil samples were collected from several thermophilic sites in the Dumat Al-Jandal and Al-Qurayyat cities. In total, 28 bacterial strains were isolated and biochemically characterized. Most isolated strains showed high protease and amylase production. 46 and 28% of isolated strains showed the production of cellulase and gelatinase, respectively. However, lipase production was not observed in any isolated bacteria. Four Bacillus licheniformis and two Bacillus subtilis strains exhibited high GC content (55%). Our findings suggest thermophilic bacteria as a source of thermostable enzymes for pharmaceutical and industrial applications. |
| first_indexed | 2024-04-24T22:52:02Z |
| format | Article |
| id | doaj.art-fa64c28d085f4ab882d6d08f3c00a3ed |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-24T22:52:02Z |
| publishDate | 2024-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-fa64c28d085f4ab882d6d08f3c00a3ed2024-03-18T10:55:16ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2024-03-01181243256https://doi.org/10.22207/JPAM.18.1.07Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi ArabiaSherifa A. SaidanRaja Jarbouihttps://orcid.org/0000-0002-2093-6450Salam S. Alshararihttps://orcid.org/0000-0001-7206-823XMona S. Azabhttps://orcid.org/0000-0003-1650-0248This study aims to investigate the morphological and biochemical characteristics of thermophilic bacteria isolated from thermophilic biotopes in the Al-Jouf region of Saudi Arabia for the first time. Additionally, the production of thermostable bacterial enzymes (proteases, amylases, cellulases, gelatinases, and lipases) was investigated. Six main bacterial species were identified via 16S rRNA sequencing and phylogenetic analysis. Twenty water and soil samples were collected from several thermophilic sites in the Dumat Al-Jandal and Al-Qurayyat cities. In total, 28 bacterial strains were isolated and biochemically characterized. Most isolated strains showed high protease and amylase production. 46 and 28% of isolated strains showed the production of cellulase and gelatinase, respectively. However, lipase production was not observed in any isolated bacteria. Four Bacillus licheniformis and two Bacillus subtilis strains exhibited high GC content (55%). Our findings suggest thermophilic bacteria as a source of thermostable enzymes for pharmaceutical and industrial applications.https://microbiologyjournal.org/characterization-and-identification-of-thermophilic-bacteria-isolated-from-different-sites-located-in-al-jouf-region-saudi-arabia/hot springssalt marsh soilbacillus licheniformisbacillus subtilisthermostable enzymes |
| spellingShingle | Sherifa A. Saidan Raja Jarboui Salam S. Alsharari Mona S. Azab Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia Journal of Pure and Applied Microbiology hot springs salt marsh soil bacillus licheniformis bacillus subtilis thermostable enzymes |
| title | Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia |
| title_full | Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia |
| title_fullStr | Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia |
| title_full_unstemmed | Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia |
| title_short | Characterization and Identification of Thermophilic Bacteria Isolated from Different Sites Located in Al-Jouf Region, Saudi Arabia |
| title_sort | characterization and identification of thermophilic bacteria isolated from different sites located in al jouf region saudi arabia |
| topic | hot springs salt marsh soil bacillus licheniformis bacillus subtilis thermostable enzymes |
| url | https://microbiologyjournal.org/characterization-and-identification-of-thermophilic-bacteria-isolated-from-different-sites-located-in-al-jouf-region-saudi-arabia/ |
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