The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair development
IntroductionZinc finger protein 3 (ZFP3) and closely related C2H2 zinc finger proteins have been identified as regulators of abscisic acid signals and photomorphogenic responses during germination. Whether ZFP3 and related ZFP factors regulate plant development is, however, not known.ResultsZFP3 ove...
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1221519/full |
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| author | Dániel Benyó Emese Bató Dóra Faragó Gábor Rigó Ildikó Domonkos Nitin Labhane Laura Zsigmond Melvin Prasad István Nagy István Nagy László Szabados |
| author_facet | Dániel Benyó Emese Bató Dóra Faragó Gábor Rigó Ildikó Domonkos Nitin Labhane Laura Zsigmond Melvin Prasad István Nagy István Nagy László Szabados |
| author_sort | Dániel Benyó |
| collection | DOAJ |
| description | IntroductionZinc finger protein 3 (ZFP3) and closely related C2H2 zinc finger proteins have been identified as regulators of abscisic acid signals and photomorphogenic responses during germination. Whether ZFP3 and related ZFP factors regulate plant development is, however, not known.ResultsZFP3 overexpression reduced plant growth, limited cell expansion in leaves, and compromised root hair development. The T-DNA insertion zfp3 mutant and transgenic lines with silenced ZFP1, ZFP3, ZFP4, and ZFP7 genes were similar to wild-type plants or had only minor differences in plant growth and morphology, probably due to functional redundancy. RNAseq transcript profiling identified ZFP3-controlled gene sets, including targets of ABA signaling with reduced transcript abundance. The largest gene set that was downregulated by ZFP3 encoded regulatory and structural proteins in cell wall biogenesis, cell differentiation, and root hair formation. Chromatin immunoprecipitation confirmed ZFP3 binding to several target promoters.DiscussionOur results suggest that ZFP3 and related ZnF proteins can modulate cellular differentiation and plant vegetative development by regulating the expression of genes implicated in cell wall biogenesis. |
| first_indexed | 2024-03-08T16:51:34Z |
| format | Article |
| id | doaj.art-fa68932f2a884833b4b418a5b5d68057 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T16:51:34Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-fa68932f2a884833b4b418a5b5d680572024-01-05T04:30:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-01-011410.3389/fpls.2023.12215191221519The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair developmentDániel Benyó0Emese Bató1Dóra Faragó2Gábor Rigó3Ildikó Domonkos4Nitin Labhane5Laura Zsigmond6Melvin Prasad7István Nagy8István Nagy9László Szabados10Instiute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryInstiute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryInstiute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryInstiute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryInstiute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryDepartment of Botany, Bhavan’s College, Mumbai, Maharashtra, IndiaInstiute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryInstiute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryInstitute of Biochemistry, HUN-REN Biological Research Centre, Szeged, HungarySeqOmics Biotechnology Ltd, Mórahalom, HungaryInstiute of Plant Biology, HUN-REN Biological Research Centre, Szeged, HungaryIntroductionZinc finger protein 3 (ZFP3) and closely related C2H2 zinc finger proteins have been identified as regulators of abscisic acid signals and photomorphogenic responses during germination. Whether ZFP3 and related ZFP factors regulate plant development is, however, not known.ResultsZFP3 overexpression reduced plant growth, limited cell expansion in leaves, and compromised root hair development. The T-DNA insertion zfp3 mutant and transgenic lines with silenced ZFP1, ZFP3, ZFP4, and ZFP7 genes were similar to wild-type plants or had only minor differences in plant growth and morphology, probably due to functional redundancy. RNAseq transcript profiling identified ZFP3-controlled gene sets, including targets of ABA signaling with reduced transcript abundance. The largest gene set that was downregulated by ZFP3 encoded regulatory and structural proteins in cell wall biogenesis, cell differentiation, and root hair formation. Chromatin immunoprecipitation confirmed ZFP3 binding to several target promoters.DiscussionOur results suggest that ZFP3 and related ZnF proteins can modulate cellular differentiation and plant vegetative development by regulating the expression of genes implicated in cell wall biogenesis.https://www.frontiersin.org/articles/10.3389/fpls.2023.1221519/fullArabidopsis thalianazinc finger protein 3gene overexpressiongene silencingplant developmentroot hair |
| spellingShingle | Dániel Benyó Emese Bató Dóra Faragó Gábor Rigó Ildikó Domonkos Nitin Labhane Laura Zsigmond Melvin Prasad István Nagy István Nagy László Szabados The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair development Frontiers in Plant Science Arabidopsis thaliana zinc finger protein 3 gene overexpression gene silencing plant development root hair |
| title | The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair development |
| title_full | The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair development |
| title_fullStr | The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair development |
| title_full_unstemmed | The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair development |
| title_short | The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair development |
| title_sort | zinc finger protein 3 of arabidopsis thaliana regulates vegetative growth and root hair development |
| topic | Arabidopsis thaliana zinc finger protein 3 gene overexpression gene silencing plant development root hair |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1221519/full |
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