A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein β subunit, AGB1.
An Arabidopsis U-box E3 ubiquitin ligase Plant U-box 20 (PUB20; alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein β subunit, AGB1, by yeast two-hybrid screening. A bimolecular fluorescence complementation (BiFC) assay showed that PUB20 interacted with...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3499536?pdf=render |
| _version_ | 1818579935317458944 |
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| author | Shio Kobayashi Daisuke Tsugama Shenkui Liu Tetsuo Takano |
| author_facet | Shio Kobayashi Daisuke Tsugama Shenkui Liu Tetsuo Takano |
| author_sort | Shio Kobayashi |
| collection | DOAJ |
| description | An Arabidopsis U-box E3 ubiquitin ligase Plant U-box 20 (PUB20; alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein β subunit, AGB1, by yeast two-hybrid screening. A bimolecular fluorescence complementation (BiFC) assay showed that PUB20 interacted with AGB1 in the nuclei and the cytosol. The expression levels of PUB20 and its closest homolog, PUB21 were stable under many conditions. GUS driven by the PUB20 promoter was active in anthers, pollen, premature seeds and receptacles and GUS driven by the PUB21 promoter was active in anthers and funiculi. PUB20 was found to have autoubiquitination activity in vitro. |
| first_indexed | 2024-12-16T07:09:36Z |
| format | Article |
| id | doaj.art-fa6a9b32e29c40faaa17b622875a6f3e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T07:09:36Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fa6a9b32e29c40faaa17b622875a6f3e2022-12-21T22:39:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e4920710.1371/journal.pone.0049207A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein β subunit, AGB1.Shio KobayashiDaisuke TsugamaShenkui LiuTetsuo TakanoAn Arabidopsis U-box E3 ubiquitin ligase Plant U-box 20 (PUB20; alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein β subunit, AGB1, by yeast two-hybrid screening. A bimolecular fluorescence complementation (BiFC) assay showed that PUB20 interacted with AGB1 in the nuclei and the cytosol. The expression levels of PUB20 and its closest homolog, PUB21 were stable under many conditions. GUS driven by the PUB20 promoter was active in anthers, pollen, premature seeds and receptacles and GUS driven by the PUB21 promoter was active in anthers and funiculi. PUB20 was found to have autoubiquitination activity in vitro.http://europepmc.org/articles/PMC3499536?pdf=render |
| spellingShingle | Shio Kobayashi Daisuke Tsugama Shenkui Liu Tetsuo Takano A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein β subunit, AGB1. PLoS ONE |
| title | A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein β subunit, AGB1. |
| title_full | A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein β subunit, AGB1. |
| title_fullStr | A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein β subunit, AGB1. |
| title_full_unstemmed | A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein β subunit, AGB1. |
| title_short | A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein β subunit, AGB1. |
| title_sort | u box e3 ubiquitin ligase pub20 interacts with the arabidopsis g protein β subunit agb1 |
| url | http://europepmc.org/articles/PMC3499536?pdf=render |
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