Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy
Background S100P is a member of the S100 family of calcium-binding proteins, and it participates in pathophysiological events, such as tumor growth and invasion. Based on the striking similarities between trophoblast cells and tumor cells with regard to proliferative and invasive properties, we rais...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2015-01-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45274_3d285ad31759deda4dea85c4fbb1aa26.pdf |
| _version_ | 1819294501391302656 |
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| author | Hai-Yan Zhu Zhu Xiao-Mei Tong Tong Xiao-Na Lin Lin Ling-Ying Jiang Jiang Jun-Xia Wang Wang Song-Ying Zhang |
| author_facet | Hai-Yan Zhu Zhu Xiao-Mei Tong Tong Xiao-Na Lin Lin Ling-Ying Jiang Jiang Jun-Xia Wang Wang Song-Ying Zhang |
| author_sort | Hai-Yan Zhu Zhu |
| collection | DOAJ |
| description | Background S100P is a member of the S100 family of calcium-binding proteins, and it participates in pathophysiological events, such as tumor growth and invasion. Based on the striking similarities between trophoblast cells and tumor cells with regard to proliferative and invasive properties, we raised the question of whether and how S100P expresses in trophoblast cells during development. This study aimed to investigate the expression pattern of S100P in the human placenta during pregnancy development. Materials and Methods In this experimental study, we collected 16 first-trimester placental tissues, 10 second-trimester placental tissues, and 12 term placentas. The mRNA expression levels of S100P were detected by reverse-transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR, the protein expression levels were detected by western blot, and the localization of S100P was measured by immunohistochemical staining. The values obtained from PCR and western blot analysis were expressed as the mean ± SD. Levene’s test was used to test equal variances, and one-way analysis of variance (ANOVA) was used to evaluate differences between groups. Results Protein and mRNA expression of S100P could be detected in placenta during pregnancy, with minor higher levels in first-trimester (p > 0.05). Immunohistochemical staining revealed that S100P protein was strongly expressed in syncytiotrophoblasts, and moderate expression was detected in villous cytotrophoblasts and cytotrophoblast columns. The S100P protein was localized to both cytoplasm and nuclei in syncytiotrophoblasts, while it only existed in the cytoplasm of cytotrophoblasts. Conclusion S100P was strongly detected in human placenta during pregnancy. The specific expression and distribution of S100P in human placenta throughout gestation suggested that S100P function might vary with its location in the placenta. |
| first_indexed | 2024-12-24T04:27:19Z |
| format | Article |
| id | doaj.art-fa6b3573c82743998400be943c16b707 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-24T04:27:19Z |
| publishDate | 2015-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-fa6b3573c82743998400be943c16b7072022-12-21T17:15:32ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782015-01-018444545210.22074/ijfs.2015.418945274Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during PregnancyHai-Yan Zhu ZhuXiao-Mei Tong TongXiao-Na Lin LinLing-Ying Jiang JiangJun-Xia Wang WangSong-Ying ZhangBackground S100P is a member of the S100 family of calcium-binding proteins, and it participates in pathophysiological events, such as tumor growth and invasion. Based on the striking similarities between trophoblast cells and tumor cells with regard to proliferative and invasive properties, we raised the question of whether and how S100P expresses in trophoblast cells during development. This study aimed to investigate the expression pattern of S100P in the human placenta during pregnancy development. Materials and Methods In this experimental study, we collected 16 first-trimester placental tissues, 10 second-trimester placental tissues, and 12 term placentas. The mRNA expression levels of S100P were detected by reverse-transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR, the protein expression levels were detected by western blot, and the localization of S100P was measured by immunohistochemical staining. The values obtained from PCR and western blot analysis were expressed as the mean ± SD. Levene’s test was used to test equal variances, and one-way analysis of variance (ANOVA) was used to evaluate differences between groups. Results Protein and mRNA expression of S100P could be detected in placenta during pregnancy, with minor higher levels in first-trimester (p > 0.05). Immunohistochemical staining revealed that S100P protein was strongly expressed in syncytiotrophoblasts, and moderate expression was detected in villous cytotrophoblasts and cytotrophoblast columns. The S100P protein was localized to both cytoplasm and nuclei in syncytiotrophoblasts, while it only existed in the cytoplasm of cytotrophoblasts. Conclusion S100P was strongly detected in human placenta during pregnancy. The specific expression and distribution of S100P in human placenta throughout gestation suggested that S100P function might vary with its location in the placenta.http://www.ijfs.ir/article_45274_3d285ad31759deda4dea85c4fbb1aa26.pdfs100pplacentatrophoblastpregnancy |
| spellingShingle | Hai-Yan Zhu Zhu Xiao-Mei Tong Tong Xiao-Na Lin Lin Ling-Ying Jiang Jiang Jun-Xia Wang Wang Song-Ying Zhang Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy International Journal of Fertility and Sterility s100p placenta trophoblast pregnancy |
| title | Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy |
| title_full | Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy |
| title_fullStr | Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy |
| title_full_unstemmed | Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy |
| title_short | Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy |
| title_sort | expression and distribution of calcium binding protein s100p in human placenta during pregnancy |
| topic | s100p placenta trophoblast pregnancy |
| url | http://www.ijfs.ir/article_45274_3d285ad31759deda4dea85c4fbb1aa26.pdf |
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