Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancer
Abstract Backgroud Pancreatic cancer (PC) is a highly invasive malignancy with extremely poor prognosis. STK31 has been identified as a cancer‐testis (CT) gene, but its function in PC has not been elucidated well. Methods The effect of STK31 on cell proliferation, migration and invasion was investig...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-03-01
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| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.5472 |
| _version_ | 1797854240359579648 |
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| author | Hao Gao Baobao Cai Zipeng Lu Guangfu Wang Yong Gao Yi Miao Kuirong Jiang Kai Zhang |
| author_facet | Hao Gao Baobao Cai Zipeng Lu Guangfu Wang Yong Gao Yi Miao Kuirong Jiang Kai Zhang |
| author_sort | Hao Gao |
| collection | DOAJ |
| description | Abstract Backgroud Pancreatic cancer (PC) is a highly invasive malignancy with extremely poor prognosis. STK31 has been identified as a cancer‐testis (CT) gene, but its function in PC has not been elucidated well. Methods The effect of STK31 on cell proliferation, migration and invasion was investigated by in vitro and in vivo experiments and total RNA sequencing and targeted bisulfite sequencing was applied to explore the potential regulatory mechanisms of STK31 in PC. Results By analysis of tissue samples and the clinicopathologic features, we found that STK31 was reactivated in PC and associated with poor prognosis. In addition, the vitro and vivo studies indicated that STK31 could promote PC progression by facilitating cell proliferation, migration and invasion, and the indication. Targeted Bisulfite Sequencing showed that STK31 was regulated by methylation. Furthermore, the results of total RNA sequencing suggested that STK31 was closely related to signal transduction, metabolism, and the immune system. Conclusions This study demonstrates that STK31, as a CT gene, can promote the development of PC and is regulated by methylation. STK31 could be considered as a potential therapeutic target for PC. |
| first_indexed | 2024-04-09T20:03:03Z |
| format | Article |
| id | doaj.art-fa6bea58163a4f449378c9487b683e59 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-04-09T20:03:03Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-fa6bea58163a4f449378c9487b683e592023-04-02T20:55:00ZengWileyCancer Medicine2045-76342023-03-011267273728210.1002/cam4.5472Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancerHao Gao0Baobao Cai1Zipeng Lu2Guangfu Wang3Yong Gao4Yi Miao5Kuirong Jiang6Kai Zhang7Pancreas Center the First Affiliated Hospital of Nanjing Medical University Nanjing ChinaPancreas Center the First Affiliated Hospital of Nanjing Medical University Nanjing ChinaPancreas Center the First Affiliated Hospital of Nanjing Medical University Nanjing ChinaPancreas Center the First Affiliated Hospital of Nanjing Medical University Nanjing ChinaPancreas Center the First Affiliated Hospital of Nanjing Medical University Nanjing ChinaPancreas Center the First Affiliated Hospital of Nanjing Medical University Nanjing ChinaPancreas Center the First Affiliated Hospital of Nanjing Medical University Nanjing ChinaPancreas Center the First Affiliated Hospital of Nanjing Medical University Nanjing ChinaAbstract Backgroud Pancreatic cancer (PC) is a highly invasive malignancy with extremely poor prognosis. STK31 has been identified as a cancer‐testis (CT) gene, but its function in PC has not been elucidated well. Methods The effect of STK31 on cell proliferation, migration and invasion was investigated by in vitro and in vivo experiments and total RNA sequencing and targeted bisulfite sequencing was applied to explore the potential regulatory mechanisms of STK31 in PC. Results By analysis of tissue samples and the clinicopathologic features, we found that STK31 was reactivated in PC and associated with poor prognosis. In addition, the vitro and vivo studies indicated that STK31 could promote PC progression by facilitating cell proliferation, migration and invasion, and the indication. Targeted Bisulfite Sequencing showed that STK31 was regulated by methylation. Furthermore, the results of total RNA sequencing suggested that STK31 was closely related to signal transduction, metabolism, and the immune system. Conclusions This study demonstrates that STK31, as a CT gene, can promote the development of PC and is regulated by methylation. STK31 could be considered as a potential therapeutic target for PC.https://doi.org/10.1002/cam4.5472CT geneimmunotherapyinvasionmigrationproliferationSTK31 |
| spellingShingle | Hao Gao Baobao Cai Zipeng Lu Guangfu Wang Yong Gao Yi Miao Kuirong Jiang Kai Zhang Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancer Cancer Medicine CT gene immunotherapy invasion migration proliferation STK31 |
| title | Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancer |
| title_full | Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancer |
| title_fullStr | Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancer |
| title_full_unstemmed | Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancer |
| title_short | Cancer‐testis gene STK31 is regulated by methylation and promotes the development of pancreatic cancer |
| title_sort | cancer testis gene stk31 is regulated by methylation and promotes the development of pancreatic cancer |
| topic | CT gene immunotherapy invasion migration proliferation STK31 |
| url | https://doi.org/10.1002/cam4.5472 |
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