Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental study
Abstract Background: Sperm quality has an important role in the success of assisted reproductive techniques, by adding some bioactive agents with a positive impact on sperms, it can be improved. Objective: This study aimed to evaluate the effects of kisspeptin on the sperm motility criteria, Lactate...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shahid Sadoughi University of Medical Sciences
2022-04-01
|
| Series: | International Journal of Reproductive BioMedicine |
| Subjects: | |
| Online Access: | https://doi.org/10.18502/ijrm.v20i4.10903 |
| _version_ | 1818252881885659136 |
|---|---|
| author | Masoumeh Akmali Roghayeh Yalmeh Tahereh Talaei-Khozani Fatemeh Karimi Elham Aliabadi |
| author_facet | Masoumeh Akmali Roghayeh Yalmeh Tahereh Talaei-Khozani Fatemeh Karimi Elham Aliabadi |
| author_sort | Masoumeh Akmali |
| collection | DOAJ |
| description | Abstract Background: Sperm quality has an important role in the success of assisted reproductive techniques, by adding some bioactive agents with a positive impact on sperms, it can be improved. Objective: This study aimed to evaluate the effects of kisspeptin on the sperm motility criteria, Lactate dehydrogenase-C (LDHC) activity, acrosomal reaction, and capacitation in the mouse testicular sperm in vitro. Materials and Methods: Sperm samples were extracted from testes of 96 male Balb/C mice weighing 25-30 gr, aged 6-8 wk. Then, they were separated into 4 parts; 2 controls and 2 kisspeptin-treated aliquots; each one incubated for either 15 or 30 min. The sperm motility and the LDHC activity were evaluated, and also the frequency of the non-capacitated, intact, and acrosomal-reacted sperms were evaluated by staining with Wheat germ agglutinin, Peanut agglutinin, and Concanavalin A, respectively. The stained sperms were analyzed by flow cytometry and fluorescent microscope. Results: Our result showed that kisspeptin increased both the sperm motility (p = 0.04) and LDHC enzyme activity (p = 0.04) after 15 min of incubation. At the same time, it did not impact the frequency of the non-capacitated, intact and acrosomal-reacted sperms after incubation in the same period (p = 0.16). Conclusion: A 15 min period of incubation with kisspeptin could be applicable for evaluating sperm motility and LDH activity. |
| first_indexed | 2024-12-12T16:31:14Z |
| format | Article |
| id | doaj.art-fa6bfa984c1d459dbc2668dd55c70904 |
| institution | Directory Open Access Journal |
| issn | 2476-4108 2476-3772 |
| language | English |
| last_indexed | 2024-12-12T16:31:14Z |
| publishDate | 2022-04-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-fa6bfa984c1d459dbc2668dd55c709042022-12-22T00:18:47ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722022-04-0120430731810.18502/ijrm.v20i4.10903ijrm.v20i4.10903Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental studyMasoumeh Akmali0Roghayeh Yalmeh1Tahereh Talaei-Khozani2Fatemeh Karimi3Elham Aliabadi4Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Abstract Background: Sperm quality has an important role in the success of assisted reproductive techniques, by adding some bioactive agents with a positive impact on sperms, it can be improved. Objective: This study aimed to evaluate the effects of kisspeptin on the sperm motility criteria, Lactate dehydrogenase-C (LDHC) activity, acrosomal reaction, and capacitation in the mouse testicular sperm in vitro. Materials and Methods: Sperm samples were extracted from testes of 96 male Balb/C mice weighing 25-30 gr, aged 6-8 wk. Then, they were separated into 4 parts; 2 controls and 2 kisspeptin-treated aliquots; each one incubated for either 15 or 30 min. The sperm motility and the LDHC activity were evaluated, and also the frequency of the non-capacitated, intact, and acrosomal-reacted sperms were evaluated by staining with Wheat germ agglutinin, Peanut agglutinin, and Concanavalin A, respectively. The stained sperms were analyzed by flow cytometry and fluorescent microscope. Results: Our result showed that kisspeptin increased both the sperm motility (p = 0.04) and LDHC enzyme activity (p = 0.04) after 15 min of incubation. At the same time, it did not impact the frequency of the non-capacitated, intact and acrosomal-reacted sperms after incubation in the same period (p = 0.16). Conclusion: A 15 min period of incubation with kisspeptin could be applicable for evaluating sperm motility and LDH activity.https://doi.org/10.18502/ijrm.v20i4.10903testis, spermatozoa, kisspeptin, lactate dehydrogenase, acrosome. |
| spellingShingle | Masoumeh Akmali Roghayeh Yalmeh Tahereh Talaei-Khozani Fatemeh Karimi Elham Aliabadi Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental study International Journal of Reproductive BioMedicine testis, spermatozoa, kisspeptin, lactate dehydrogenase, acrosome. |
| title | Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental study |
| title_full | Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental study |
| title_fullStr | Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental study |
| title_full_unstemmed | Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental study |
| title_short | Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental study |
| title_sort | effects of kisspeptin incubation on the mature mouse testicular sperms quality an experimental study |
| topic | testis, spermatozoa, kisspeptin, lactate dehydrogenase, acrosome. |
| url | https://doi.org/10.18502/ijrm.v20i4.10903 |
| work_keys_str_mv | AT masoumehakmali effectsofkisspeptinincubationonthematuremousetesticularspermsqualityanexperimentalstudy AT roghayehyalmeh effectsofkisspeptinincubationonthematuremousetesticularspermsqualityanexperimentalstudy AT taherehtalaeikhozani effectsofkisspeptinincubationonthematuremousetesticularspermsqualityanexperimentalstudy AT fatemehkarimi effectsofkisspeptinincubationonthematuremousetesticularspermsqualityanexperimentalstudy AT elhamaliabadi effectsofkisspeptinincubationonthematuremousetesticularspermsqualityanexperimentalstudy |