Acaricidal effect of Momordica charantia, Megaskepasma erythrochlamys and Gliricidia sepium on the Rhipicephalus microplus
Objective. The acaricidal activity of Momordica charantia (Mc), Megaskepasma erythrochlamys (Me) and Gliricidia sepium (Gs) on Rhipicephalus microplus (Rm) was evaluated. Materials and methods. Preliminary phytochemical analysis of leaves of the methanolic extract of Mc (EMc), the ethanolic extract...
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| Format: | Article |
| Language: | English |
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Universidad de Cordoba
2019-12-01
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| Series: | Revista MVZ Cordoba |
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| Online Access: | https://revistamvz.unicordoba.edu.co/article/view/e1951/2376 |
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| author | Dumar Jaramillo H Angélica González R Natalia Pedraza C Jorge Sierra A Gina García M Ricardo Jara A |
| author_facet | Dumar Jaramillo H Angélica González R Natalia Pedraza C Jorge Sierra A Gina García M Ricardo Jara A |
| author_sort | Dumar Jaramillo H |
| collection | DOAJ |
| description | Objective. The acaricidal activity of Momordica charantia (Mc), Megaskepasma erythrochlamys (Me) and Gliricidia sepium (Gs) on Rhipicephalus microplus (Rm) was evaluated. Materials and methods. Preliminary phytochemical analysis of leaves of the methanolic extract of Mc (EMc), the ethanolic extract of Me (EMe) and the acetone extract of Gs (EGs) were carried out through the technique of colorimetry and thin layer chromatography (CCD). The acaricidal activity was
performed through in-vitro tests using the larval immersion test (LIT) and the adult immersion test
(AIT). For in-situ tests, grazing cattle naturally infested with ticks were used, using the LC50 obtained from the in-vitro AIT tests; subsequently engorged females were taken to incubation to assess their reproductive capacity Results. The presence of several groups of secondary metabolites of acaricidal interest was determined. The acaricidal effect of plants extracts on engorged females was demonstrated; although only EGs showed larvicidal activity. Extracts at 160 mg/mL affected the life cycle of Rm by inhibiting oviposition in 46.9%, 66.1% and 84.03% (p<0.05) for EGs, EMc and EMe, respectively. On the other hand, the in-situ testing, significant difference (p<0.05) was observed between EMc and EMe regarding control groups. Conclusions. The results obtained are promising to strengthen the possibility of linking the extracts of these plants within integrated plans to control
ticks in cattle production systems. |
| first_indexed | 2024-12-19T21:49:41Z |
| format | Article |
| id | doaj.art-fa6cd1bdf4a34e20bec169a51bad6fb1 |
| institution | Directory Open Access Journal |
| issn | 0122-0268 1909-0544 |
| language | English |
| last_indexed | 2024-12-19T21:49:41Z |
| publishDate | 2019-12-01 |
| publisher | Universidad de Cordoba |
| record_format | Article |
| series | Revista MVZ Cordoba |
| spelling | doaj.art-fa6cd1bdf4a34e20bec169a51bad6fb12022-12-21T20:04:26ZengUniversidad de CordobaRevista MVZ Cordoba0122-02681909-05442019-12-01251e1951https://doi.org/10.21897/rmvz.1951Acaricidal effect of Momordica charantia, Megaskepasma erythrochlamys and Gliricidia sepium on the Rhipicephalus microplusDumar Jaramillo H0https://orcid.org/0000-0003-1377-1747Angélica González R1https://orcid.org/0000-0001-7258-0989Natalia Pedraza C2https://orcid.org/0000-0001-5991-0525Jorge Sierra A3https://orcid.org/0000-0002-8244-9260Gina García M4https://orcid.org/0000-0001-5840-3302Ricardo Jara A5https://orcid.org/0000-0002-6079-4733Universidad de los Llanos, Facultad de Ciencias Agropecuarias y Recursos Naturales, Escuela de Ciencias Animales, Research group in Experimental Pharmacology and internal medicine -ÉLITE, Km 12 Vía Puerto López Villavicencio, Colombia.Universidad de los Llanos, Facultad de Ciencias Agropecuarias y Recursos Naturales, Escuela de Ciencias Animales, Research group in Experimental Pharmacology and internal medicine -ÉLITE, Km 12 Vía Puerto López Villavicencio, Colombia.Universidad de los Llanos, Facultad de Ciencias Agropecuarias y Recursos Naturales, Escuela de Ciencias Animales, Research group in Experimental Pharmacology and internal medicine -ÉLITE, Km 12 Vía Puerto López Villavicencio, Colombia.Universidad de los Llanos, Facultad de Ciencias Básicas e Ingeniería, Departamento de Química. Research group in Experimental Pharmacology and Internal Medicine –ÉLITE.Universidad de los Llanos, Facultad de Ciencias Agropecuarias y Recursos Naturales, Escuela de Ciencias Animales, Research group in Experimental Pharmacology and internal medicine -ÉLITE, Km 12 Vía Puerto López Villavicencio, Colombia.Universidad de los Llanos, Facultad de Ciencias Agropecuarias y Recursos Naturales, Escuela de Ciencias Animales, Research group in Experimental Pharmacology and internal medicine -ÉLITE, Km 12 Vía Puerto López Villavicencio, Colombia.Objective. The acaricidal activity of Momordica charantia (Mc), Megaskepasma erythrochlamys (Me) and Gliricidia sepium (Gs) on Rhipicephalus microplus (Rm) was evaluated. Materials and methods. Preliminary phytochemical analysis of leaves of the methanolic extract of Mc (EMc), the ethanolic extract of Me (EMe) and the acetone extract of Gs (EGs) were carried out through the technique of colorimetry and thin layer chromatography (CCD). The acaricidal activity was performed through in-vitro tests using the larval immersion test (LIT) and the adult immersion test (AIT). For in-situ tests, grazing cattle naturally infested with ticks were used, using the LC50 obtained from the in-vitro AIT tests; subsequently engorged females were taken to incubation to assess their reproductive capacity Results. The presence of several groups of secondary metabolites of acaricidal interest was determined. The acaricidal effect of plants extracts on engorged females was demonstrated; although only EGs showed larvicidal activity. Extracts at 160 mg/mL affected the life cycle of Rm by inhibiting oviposition in 46.9%, 66.1% and 84.03% (p<0.05) for EGs, EMc and EMe, respectively. On the other hand, the in-situ testing, significant difference (p<0.05) was observed between EMc and EMe regarding control groups. Conclusions. The results obtained are promising to strengthen the possibility of linking the extracts of these plants within integrated plans to control ticks in cattle production systems.https://revistamvz.unicordoba.edu.co/article/view/e1951/2376acaricattle diseasesethnopharmacology |
| spellingShingle | Dumar Jaramillo H Angélica González R Natalia Pedraza C Jorge Sierra A Gina García M Ricardo Jara A Acaricidal effect of Momordica charantia, Megaskepasma erythrochlamys and Gliricidia sepium on the Rhipicephalus microplus Revista MVZ Cordoba acari cattle diseases ethnopharmacology |
| title | Acaricidal effect of Momordica charantia, Megaskepasma erythrochlamys and Gliricidia sepium on the Rhipicephalus microplus |
| title_full | Acaricidal effect of Momordica charantia, Megaskepasma erythrochlamys and Gliricidia sepium on the Rhipicephalus microplus |
| title_fullStr | Acaricidal effect of Momordica charantia, Megaskepasma erythrochlamys and Gliricidia sepium on the Rhipicephalus microplus |
| title_full_unstemmed | Acaricidal effect of Momordica charantia, Megaskepasma erythrochlamys and Gliricidia sepium on the Rhipicephalus microplus |
| title_short | Acaricidal effect of Momordica charantia, Megaskepasma erythrochlamys and Gliricidia sepium on the Rhipicephalus microplus |
| title_sort | acaricidal effect of momordica charantia megaskepasma erythrochlamys and gliricidia sepium on the rhipicephalus microplus |
| topic | acari cattle diseases ethnopharmacology |
| url | https://revistamvz.unicordoba.edu.co/article/view/e1951/2376 |
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