Characterization of Maize miRNAs in Response to Synergistic Infection of Maize Chlorotic Mottle Virus and Sugarcane Mosaic Virus
The synergistic infection of maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) causes maize lethal necrosis, with considerable losses to global maize production. microRNAs (miRNAs) are conserved non-coding small RNAs that play essential regulatory roles in plant development and e...
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| Format: | Article |
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MDPI AG
2019-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/20/13/3146 |
| _version_ | 1811191728290398208 |
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| author | Zihao Xia Zhenxing Zhao Xinran Gao Zhiyuan Jiao Yuanhua Wu Tao Zhou Zaifeng Fan |
| author_facet | Zihao Xia Zhenxing Zhao Xinran Gao Zhiyuan Jiao Yuanhua Wu Tao Zhou Zaifeng Fan |
| author_sort | Zihao Xia |
| collection | DOAJ |
| description | The synergistic infection of maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) causes maize lethal necrosis, with considerable losses to global maize production. microRNAs (miRNAs) are conserved non-coding small RNAs that play essential regulatory roles in plant development and environmental stress responses, including virus infection. However, the characterization of maize miRNAs in response to synergistic infection of MCMV and SCMV remains largely unknown. In this study, the profiles of small RNAs from MCMV and SCMV single- and co-infected (S + M) maize plants were obtained by high-throughput sequencing. A total of 173 known miRNAs, belonging to 26 miRNA families, and 49 novel miRNAs were profiled. The expression patterns of most miRNAs in S + M-infected maize plants were similar to that in SCMV-infected maize plants, probably due to the existence of RNA silencing suppressor HC-Pro. Northern blotting and quantitative real-time PCR were performed to validate the accumulation of miRNAs and their targets in different experimental treatments, respectively. The down-regulation of miR159, miR393, and miR394 might be involved in antiviral defense to synergistic infection. These results provide novel insights into the regulatory networks of miRNAs in maize plants in response to the synergistic infection of MCMV and SCMV. |
| first_indexed | 2024-04-11T23:40:42Z |
| format | Article |
| id | doaj.art-fa6efe8621fb4f31b2828d59b87c1b96 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-11T23:40:42Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fa6efe8621fb4f31b2828d59b87c1b962022-12-22T03:56:48ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-06-012013314610.3390/ijms20133146ijms20133146Characterization of Maize miRNAs in Response to Synergistic Infection of Maize Chlorotic Mottle Virus and Sugarcane Mosaic VirusZihao Xia0Zhenxing Zhao1Xinran Gao2Zhiyuan Jiao3Yuanhua Wu4Tao Zhou5Zaifeng Fan6College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, ChinaState Key Laboratory of Agro-Biotechnology and Key Laboratory of Pest Monitoring and Green Management-MOA, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, Shenyang Agricultural University, Shenyang 110866, ChinaState Key Laboratory of Agro-Biotechnology and Key Laboratory of Pest Monitoring and Green Management-MOA, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, Shenyang Agricultural University, Shenyang 110866, ChinaState Key Laboratory of Agro-Biotechnology and Key Laboratory of Pest Monitoring and Green Management-MOA, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Agro-Biotechnology and Key Laboratory of Pest Monitoring and Green Management-MOA, China Agricultural University, Beijing 100193, ChinaThe synergistic infection of maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) causes maize lethal necrosis, with considerable losses to global maize production. microRNAs (miRNAs) are conserved non-coding small RNAs that play essential regulatory roles in plant development and environmental stress responses, including virus infection. However, the characterization of maize miRNAs in response to synergistic infection of MCMV and SCMV remains largely unknown. In this study, the profiles of small RNAs from MCMV and SCMV single- and co-infected (S + M) maize plants were obtained by high-throughput sequencing. A total of 173 known miRNAs, belonging to 26 miRNA families, and 49 novel miRNAs were profiled. The expression patterns of most miRNAs in S + M-infected maize plants were similar to that in SCMV-infected maize plants, probably due to the existence of RNA silencing suppressor HC-Pro. Northern blotting and quantitative real-time PCR were performed to validate the accumulation of miRNAs and their targets in different experimental treatments, respectively. The down-regulation of miR159, miR393, and miR394 might be involved in antiviral defense to synergistic infection. These results provide novel insights into the regulatory networks of miRNAs in maize plants in response to the synergistic infection of MCMV and SCMV.https://www.mdpi.com/1422-0067/20/13/3146maizemiRNAmaize chlorotic mottle virus (MCMV)sugarcane mosaic virus (SCMV) |
| spellingShingle | Zihao Xia Zhenxing Zhao Xinran Gao Zhiyuan Jiao Yuanhua Wu Tao Zhou Zaifeng Fan Characterization of Maize miRNAs in Response to Synergistic Infection of Maize Chlorotic Mottle Virus and Sugarcane Mosaic Virus International Journal of Molecular Sciences maize miRNA maize chlorotic mottle virus (MCMV) sugarcane mosaic virus (SCMV) |
| title | Characterization of Maize miRNAs in Response to Synergistic Infection of Maize Chlorotic Mottle Virus and Sugarcane Mosaic Virus |
| title_full | Characterization of Maize miRNAs in Response to Synergistic Infection of Maize Chlorotic Mottle Virus and Sugarcane Mosaic Virus |
| title_fullStr | Characterization of Maize miRNAs in Response to Synergistic Infection of Maize Chlorotic Mottle Virus and Sugarcane Mosaic Virus |
| title_full_unstemmed | Characterization of Maize miRNAs in Response to Synergistic Infection of Maize Chlorotic Mottle Virus and Sugarcane Mosaic Virus |
| title_short | Characterization of Maize miRNAs in Response to Synergistic Infection of Maize Chlorotic Mottle Virus and Sugarcane Mosaic Virus |
| title_sort | characterization of maize mirnas in response to synergistic infection of maize chlorotic mottle virus and sugarcane mosaic virus |
| topic | maize miRNA maize chlorotic mottle virus (MCMV) sugarcane mosaic virus (SCMV) |
| url | https://www.mdpi.com/1422-0067/20/13/3146 |
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