Establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCs
Abstract Myotonic dystrophy type 1 (DM1) is caused by expanded CTG repeats (CTGexp) in the dystrophia myotonica protein kinase (DMPK) gene, and the transcription products, expanded CUG repeats, sequester muscleblind like splicing regulator 1 (MBNL1), resulting in the nuclear MBNL1 aggregation in the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-26614-z |
| _version_ | 1797952589682180096 |
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| author | Ryu Kawada Tatsuya Jonouchi Akihiro Kagita Masae Sato Akitsu Hotta Hidetoshi Sakurai |
| author_facet | Ryu Kawada Tatsuya Jonouchi Akihiro Kagita Masae Sato Akitsu Hotta Hidetoshi Sakurai |
| author_sort | Ryu Kawada |
| collection | DOAJ |
| description | Abstract Myotonic dystrophy type 1 (DM1) is caused by expanded CTG repeats (CTGexp) in the dystrophia myotonica protein kinase (DMPK) gene, and the transcription products, expanded CUG repeats, sequester muscleblind like splicing regulator 1 (MBNL1), resulting in the nuclear MBNL1 aggregation in the DM1 cells. Loss of MBNL1 function is the pivotal mechanism underlying the pathogenesis of DM1. To develop therapeutics for DM1, proper human in vitro models based on the pathologic mechanism of DM1 are required. In this study, we established robust in vitro skeletal muscle cell models of DM1 with patient-derived induced pluripotent stem cells (iPSCs) using the MyoD1-induced system and iPSCs-derived muscle stem cell (iMuSC) differentiation system. Our newly established DM1 models enable simple quantitative evaluation of nuclear MBNL1 aggregation and the downstream splicing defects. Quantitative analyses using the MyoD1-induced myotubes showed that CTGexp-deleted DM1 skeletal myotubes exhibited a reversal of MBNL1-related pathologies, and antisense oligonucleotide treatment recovered these disease phenotypes in the DM1-iPSCs-derived myotubes. Furthermore, iMuSC-derived myotubes exhibited higher maturity than the MyoD1-induced myotubes, which enabled us to recapitulate the SERCA1 splicing defect in the DM1-iMuSC-derived myotubes. Our quantitative and reproducible in vitro models for DM1 established using human iPSCs are promising for drug discovery against DM1. |
| first_indexed | 2024-04-10T22:48:48Z |
| format | Article |
| id | doaj.art-fa71afad8748492e95365ac375fec8b3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T22:48:48Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fa71afad8748492e95365ac375fec8b32023-01-15T12:10:47ZengNature PortfolioScientific Reports2045-23222023-01-0113111710.1038/s41598-022-26614-zEstablishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCsRyu Kawada0Tatsuya Jonouchi1Akihiro Kagita2Masae Sato3Akitsu Hotta4Hidetoshi Sakurai5Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto UniversityDepartment of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto UniversityDepartment of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto UniversityDepartment of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto UniversityDepartment of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto UniversityDepartment of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto UniversityAbstract Myotonic dystrophy type 1 (DM1) is caused by expanded CTG repeats (CTGexp) in the dystrophia myotonica protein kinase (DMPK) gene, and the transcription products, expanded CUG repeats, sequester muscleblind like splicing regulator 1 (MBNL1), resulting in the nuclear MBNL1 aggregation in the DM1 cells. Loss of MBNL1 function is the pivotal mechanism underlying the pathogenesis of DM1. To develop therapeutics for DM1, proper human in vitro models based on the pathologic mechanism of DM1 are required. In this study, we established robust in vitro skeletal muscle cell models of DM1 with patient-derived induced pluripotent stem cells (iPSCs) using the MyoD1-induced system and iPSCs-derived muscle stem cell (iMuSC) differentiation system. Our newly established DM1 models enable simple quantitative evaluation of nuclear MBNL1 aggregation and the downstream splicing defects. Quantitative analyses using the MyoD1-induced myotubes showed that CTGexp-deleted DM1 skeletal myotubes exhibited a reversal of MBNL1-related pathologies, and antisense oligonucleotide treatment recovered these disease phenotypes in the DM1-iPSCs-derived myotubes. Furthermore, iMuSC-derived myotubes exhibited higher maturity than the MyoD1-induced myotubes, which enabled us to recapitulate the SERCA1 splicing defect in the DM1-iMuSC-derived myotubes. Our quantitative and reproducible in vitro models for DM1 established using human iPSCs are promising for drug discovery against DM1.https://doi.org/10.1038/s41598-022-26614-z |
| spellingShingle | Ryu Kawada Tatsuya Jonouchi Akihiro Kagita Masae Sato Akitsu Hotta Hidetoshi Sakurai Establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCs Scientific Reports |
| title | Establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCs |
| title_full | Establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCs |
| title_fullStr | Establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCs |
| title_full_unstemmed | Establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCs |
| title_short | Establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCs |
| title_sort | establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient derived ipscs |
| url | https://doi.org/10.1038/s41598-022-26614-z |
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