Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections
Staphylococcus aureus is an important causative pathogen of bloodstream infections. An amplification assay such as real-time PCR is a sensitive, specific technique to detect S. aureus. However, it needs well-trained personnel, and costs are high. A literature review focusing on rapid and simple meth...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2022-12-01
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| Series: | Polish Journal of Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.33073/pjm-2022-050 |
| _version_ | 1797947721620914176 |
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| author | Duan Rui Wang Pei |
| author_facet | Duan Rui Wang Pei |
| author_sort | Duan Rui |
| collection | DOAJ |
| description | Staphylococcus aureus is an important causative pathogen of bloodstream infections. An amplification assay such as real-time PCR is a sensitive, specific technique to detect S. aureus. However, it needs well-trained personnel, and costs are high. A literature review focusing on rapid and simple methods for diagnosing S. aureus was performed. The following methods were included: (a) Hybrisep in situ hybridization test, (b) T2Dx system, (c) BinaxNow Staphylococcus aureus and PBP2a, (d) Gram staining, (e) PNA FISH and QuickFISH, (f) Accelerate PhenoTM system, (g) MALDI-TOF MS, (h) BioFire FilmArray, (i) Xpert MRSA/SA. These rapid and simple methods can rapidly identify S. aureus in positive blood cultures or direct blood samples. Furthermore, BioFire FilmArray and Xpert MRSA/SA identify methicillin-resistant S. aureus (MRSA), and the Accelerate PhenoTM system can also provide antimicrobial susceptibility testing (AST) results. The rapidity and simplicity of results generated by these methods have the potential to improve patient outcomes and aid in the prevention of the emergence and transmission of MRSA. |
| first_indexed | 2024-04-10T21:32:15Z |
| format | Article |
| id | doaj.art-fa730577e4cd4becb6474c1b484e6ffd |
| institution | Directory Open Access Journal |
| issn | 2544-4646 |
| language | English |
| last_indexed | 2024-04-10T21:32:15Z |
| publishDate | 2022-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-fa730577e4cd4becb6474c1b484e6ffd2023-01-19T13:20:30ZengSciendoPolish Journal of Microbiology2544-46462022-12-0171448148910.33073/pjm-2022-050Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream InfectionsDuan Rui0Wang Pei1Department of Laboratory Medicine and Blood Transfusion, The First People’s Hospital of Jingmen, Jingmen, Hubei Province, ChinaDepartment of Laboratory Medicine and Blood Transfusion, The First People’s Hospital of Jingmen, Jingmen, Hubei Province, ChinaStaphylococcus aureus is an important causative pathogen of bloodstream infections. An amplification assay such as real-time PCR is a sensitive, specific technique to detect S. aureus. However, it needs well-trained personnel, and costs are high. A literature review focusing on rapid and simple methods for diagnosing S. aureus was performed. The following methods were included: (a) Hybrisep in situ hybridization test, (b) T2Dx system, (c) BinaxNow Staphylococcus aureus and PBP2a, (d) Gram staining, (e) PNA FISH and QuickFISH, (f) Accelerate PhenoTM system, (g) MALDI-TOF MS, (h) BioFire FilmArray, (i) Xpert MRSA/SA. These rapid and simple methods can rapidly identify S. aureus in positive blood cultures or direct blood samples. Furthermore, BioFire FilmArray and Xpert MRSA/SA identify methicillin-resistant S. aureus (MRSA), and the Accelerate PhenoTM system can also provide antimicrobial susceptibility testing (AST) results. The rapidity and simplicity of results generated by these methods have the potential to improve patient outcomes and aid in the prevention of the emergence and transmission of MRSA.https://doi.org/10.33073/pjm-2022-050bloodstream infectionsstaphylococcus aureusrapid diagnosticsantimicrobial stewardshipbacteremia |
| spellingShingle | Duan Rui Wang Pei Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections Polish Journal of Microbiology bloodstream infections staphylococcus aureus rapid diagnostics antimicrobial stewardship bacteremia |
| title | Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections |
| title_full | Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections |
| title_fullStr | Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections |
| title_full_unstemmed | Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections |
| title_short | Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections |
| title_sort | rapid and simple approaches for diagnosis of staphylococcus aureus in bloodstream infections |
| topic | bloodstream infections staphylococcus aureus rapid diagnostics antimicrobial stewardship bacteremia |
| url | https://doi.org/10.33073/pjm-2022-050 |
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