Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study
Background In vitro maturation (IVM) of immature oocytes retrieved from ovarian tissue has been considered as a valuable approach for fertility preservation in cancerous patients. Objective To evaluate the efficacy of vitrification on oocyte maturation, survival rates, as well as the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Sciences
2019-03-01
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| Series: | International Journal of Reproductive BioMedicine |
| Subjects: | |
| Online Access: | https://doi.org/10.18502/ijrm.v17i3.4516 |
| _version_ | 1818601285492932608 |
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| author | Mehdi Mohsenzadeh Nasim Tabibnejad Mahboubeh Vatanparast Fatemeh Anbari Mohammad Ali Khalili Mojgan Karimi-Zarchi |
| author_facet | Mehdi Mohsenzadeh Nasim Tabibnejad Mahboubeh Vatanparast Fatemeh Anbari Mohammad Ali Khalili Mojgan Karimi-Zarchi |
| author_sort | Mehdi Mohsenzadeh |
| collection | DOAJ |
| description | Background
In vitro maturation (IVM) of immature oocytes retrieved from ovarian tissue has been considered as a valuable approach for fertility preservation in cancerous patients.
Objective
To evaluate the efficacy of vitrification on oocyte maturation, survival rates, as well as the subcellular oocyte quality post IVM.
Materials and Methods
The ovarian cortexes from 19 women with cervix and uterine malignancy aged 21–39 yr were collected. Cumulus-oocyte complexes were aspirated from all visible antral follicles. 102 immature oocytes were collected, and 43 oocytes were detected appropriately for IVM (control group). Also, 59 immature oocytes were vitrified, then matured in vitro (IVM) in two groups: with Growth/differentiation factor 9 (GDF9) (group 1) and without GDF9 (group 2) supplementation. Rates of oocytes viability, maturation, and survival along with meiotic spindle visualization and zona pellucida birefringence were assessed with Polyscope.
Results
The rate of maturation was significantly higher in controls (55.8%) compared to the other groups. Maturation rate was 23.3% in oocytes cultured in IVM medium enriched with GDF9, and 27.6% in those cultured in IVM medium lacking GDF9 (p = 0.86). Also, the meiotic spindle was present in 74.4% of control oocytes which was significantly higher than the other groups. The proportion of high zona pellucida birefringence was higher in the controls when compared with group 1 (51.2% vs. 23.3%, respectively, p = 0.04).
Conclusion
Vitrification had a detrimental effect on oocyte maturation, viability as well as the subcellular quality of the oocytes after IVM in cancerous women. |
| first_indexed | 2024-12-16T12:48:57Z |
| format | Article |
| id | doaj.art-fa77aeb3f14547ea8138ffaaa3193873 |
| institution | Directory Open Access Journal |
| issn | 2476-3772 |
| language | English |
| last_indexed | 2024-12-16T12:48:57Z |
| publishDate | 2019-03-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-fa77aeb3f14547ea8138ffaaa31938732022-12-21T22:31:12ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-37722019-03-011717518410.18502/ijrm.v17i3.4516Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental studyMehdi Mohsenzadeh0Nasim Tabibnejad1Mahboubeh Vatanparast2Fatemeh Anbari3Mohammad Ali Khalili4Mojgan Karimi-Zarchi5Iran University of Medical Sciences, Tehran, Iran.Iran University of Medical Sciences, Tehran, Iran.Iran University of Medical Sciences, Tehran, Iran.Iran University of Medical Sciences, Tehran, Iran.Iran University of Medical Sciences, Tehran, Iran.Iran University of Medical Sciences, Tehran, Iran.Background In vitro maturation (IVM) of immature oocytes retrieved from ovarian tissue has been considered as a valuable approach for fertility preservation in cancerous patients. Objective To evaluate the efficacy of vitrification on oocyte maturation, survival rates, as well as the subcellular oocyte quality post IVM. Materials and Methods The ovarian cortexes from 19 women with cervix and uterine malignancy aged 21–39 yr were collected. Cumulus-oocyte complexes were aspirated from all visible antral follicles. 102 immature oocytes were collected, and 43 oocytes were detected appropriately for IVM (control group). Also, 59 immature oocytes were vitrified, then matured in vitro (IVM) in two groups: with Growth/differentiation factor 9 (GDF9) (group 1) and without GDF9 (group 2) supplementation. Rates of oocytes viability, maturation, and survival along with meiotic spindle visualization and zona pellucida birefringence were assessed with Polyscope. Results The rate of maturation was significantly higher in controls (55.8%) compared to the other groups. Maturation rate was 23.3% in oocytes cultured in IVM medium enriched with GDF9, and 27.6% in those cultured in IVM medium lacking GDF9 (p = 0.86). Also, the meiotic spindle was present in 74.4% of control oocytes which was significantly higher than the other groups. The proportion of high zona pellucida birefringence was higher in the controls when compared with group 1 (51.2% vs. 23.3%, respectively, p = 0.04). Conclusion Vitrification had a detrimental effect on oocyte maturation, viability as well as the subcellular quality of the oocytes after IVM in cancerous women.https://doi.org/10.18502/ijrm.v17i3.4516ivmvitrificationfertility preservationmaturation ratemeiotic spindlezona pellucida birefringence. |
| spellingShingle | Mehdi Mohsenzadeh Nasim Tabibnejad Mahboubeh Vatanparast Fatemeh Anbari Mohammad Ali Khalili Mojgan Karimi-Zarchi Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study International Journal of Reproductive BioMedicine ivm vitrification fertility preservation maturation rate meiotic spindle zona pellucida birefringence. |
| title | Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study |
| title_full | Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study |
| title_fullStr | Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study |
| title_full_unstemmed | Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study |
| title_short | Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study |
| title_sort | vitrification has detrimental effects on maturation viability and subcellular quality of oocytes post ivm in cancerous women an experimental study |
| topic | ivm vitrification fertility preservation maturation rate meiotic spindle zona pellucida birefringence. |
| url | https://doi.org/10.18502/ijrm.v17i3.4516 |
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