Cells Deficient in the Fanconi Anemia Protein FANCD2 are Hypersensitive to the Cytotoxicity and DNA Damage Induced by Coffee and Caffeic Acid
Epidemiological studies have found a positive association between coffee consumption and a lower risk of cardiovascular disorders, some cancers, diabetes, Parkinson and Alzheimer disease. Coffee consumption, however, has also been linked to an increased risk of developing some types of cancer, inclu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-07-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/8/7/211 |
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| author | Estefanía Burgos-Morón José Manuel Calderón-Montaño Manuel Luis Orta Emilio Guillén-Mancina Santiago Mateos Miguel López-Lázaro |
| author_facet | Estefanía Burgos-Morón José Manuel Calderón-Montaño Manuel Luis Orta Emilio Guillén-Mancina Santiago Mateos Miguel López-Lázaro |
| author_sort | Estefanía Burgos-Morón |
| collection | DOAJ |
| description | Epidemiological studies have found a positive association between coffee consumption and a lower risk of cardiovascular disorders, some cancers, diabetes, Parkinson and Alzheimer disease. Coffee consumption, however, has also been linked to an increased risk of developing some types of cancer, including bladder cancer in adults and leukemia in children of mothers who drink coffee during pregnancy. Since cancer is driven by the accumulation of DNA alterations, the ability of the coffee constituent caffeic acid to induce DNA damage in cells may play a role in the carcinogenic potential of this beverage. This carcinogenic potential may be exacerbated in cells with DNA repair defects. People with the genetic disease Fanconi Anemia have DNA repair deficiencies and are predisposed to several cancers, particularly acute myeloid leukemia. Defects in the DNA repair protein Fanconi Anemia D2 (FANCD2) also play an important role in the development of a variety of cancers (e.g., bladder cancer) in people without this genetic disease. This communication shows that cells deficient in FANCD2 are hypersensitive to the cytotoxicity (clonogenic assay) and DNA damage (γ-H2AX and 53BP1 focus assay) induced by caffeic acid and by a commercial lyophilized coffee extract. These data suggest that people with Fanconi Anemia, or healthy people who develop sporadic mutations in FANCD2, may be hypersensitive to the carcinogenic activity of coffee. |
| first_indexed | 2024-04-11T13:39:17Z |
| format | Article |
| id | doaj.art-fa79cd7512a74ef9bfe9d385b6dc14a3 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T13:39:17Z |
| publishDate | 2016-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-fa79cd7512a74ef9bfe9d385b6dc14a32022-12-22T04:21:22ZengMDPI AGToxins2072-66512016-07-018721110.3390/toxins8070211toxins8070211Cells Deficient in the Fanconi Anemia Protein FANCD2 are Hypersensitive to the Cytotoxicity and DNA Damage Induced by Coffee and Caffeic AcidEstefanía Burgos-Morón0José Manuel Calderón-Montaño1Manuel Luis Orta2Emilio Guillén-Mancina3Santiago Mateos4Miguel López-Lázaro5Department of Pharmacology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012 Seville, SpainDepartment of Pharmacology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012 Seville, SpainDepartment of Cell Biology, Faculty of Biology, University of Seville, Avda. Reina Mercedes s/n., 41012 Seville, SpainDepartment of Pharmacology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012 Seville, SpainDepartment of Cell Biology, Faculty of Biology, University of Seville, Avda. Reina Mercedes s/n., 41012 Seville, SpainDepartment of Pharmacology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012 Seville, SpainEpidemiological studies have found a positive association between coffee consumption and a lower risk of cardiovascular disorders, some cancers, diabetes, Parkinson and Alzheimer disease. Coffee consumption, however, has also been linked to an increased risk of developing some types of cancer, including bladder cancer in adults and leukemia in children of mothers who drink coffee during pregnancy. Since cancer is driven by the accumulation of DNA alterations, the ability of the coffee constituent caffeic acid to induce DNA damage in cells may play a role in the carcinogenic potential of this beverage. This carcinogenic potential may be exacerbated in cells with DNA repair defects. People with the genetic disease Fanconi Anemia have DNA repair deficiencies and are predisposed to several cancers, particularly acute myeloid leukemia. Defects in the DNA repair protein Fanconi Anemia D2 (FANCD2) also play an important role in the development of a variety of cancers (e.g., bladder cancer) in people without this genetic disease. This communication shows that cells deficient in FANCD2 are hypersensitive to the cytotoxicity (clonogenic assay) and DNA damage (γ-H2AX and 53BP1 focus assay) induced by caffeic acid and by a commercial lyophilized coffee extract. These data suggest that people with Fanconi Anemia, or healthy people who develop sporadic mutations in FANCD2, may be hypersensitive to the carcinogenic activity of coffee.http://www.mdpi.com/2072-6651/8/7/211coffeecaffeic acidcancerDNA damagecarcinogenesisFANCD2Fanconi anemia |
| spellingShingle | Estefanía Burgos-Morón José Manuel Calderón-Montaño Manuel Luis Orta Emilio Guillén-Mancina Santiago Mateos Miguel López-Lázaro Cells Deficient in the Fanconi Anemia Protein FANCD2 are Hypersensitive to the Cytotoxicity and DNA Damage Induced by Coffee and Caffeic Acid Toxins coffee caffeic acid cancer DNA damage carcinogenesis FANCD2 Fanconi anemia |
| title | Cells Deficient in the Fanconi Anemia Protein FANCD2 are Hypersensitive to the Cytotoxicity and DNA Damage Induced by Coffee and Caffeic Acid |
| title_full | Cells Deficient in the Fanconi Anemia Protein FANCD2 are Hypersensitive to the Cytotoxicity and DNA Damage Induced by Coffee and Caffeic Acid |
| title_fullStr | Cells Deficient in the Fanconi Anemia Protein FANCD2 are Hypersensitive to the Cytotoxicity and DNA Damage Induced by Coffee and Caffeic Acid |
| title_full_unstemmed | Cells Deficient in the Fanconi Anemia Protein FANCD2 are Hypersensitive to the Cytotoxicity and DNA Damage Induced by Coffee and Caffeic Acid |
| title_short | Cells Deficient in the Fanconi Anemia Protein FANCD2 are Hypersensitive to the Cytotoxicity and DNA Damage Induced by Coffee and Caffeic Acid |
| title_sort | cells deficient in the fanconi anemia protein fancd2 are hypersensitive to the cytotoxicity and dna damage induced by coffee and caffeic acid |
| topic | coffee caffeic acid cancer DNA damage carcinogenesis FANCD2 Fanconi anemia |
| url | http://www.mdpi.com/2072-6651/8/7/211 |
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