Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat

The aim of this study was to evaluate the role of chronic cadmium exposure in modulating cardiac matrix metalloproteinases (MMPs) in the heart of rats. Adult male Sprague-Dawley rats were exposed to 15 ppm CdCl2 in drinking water for 10 weeks followed by withdrawal of cadmium treatment for 4 weeks....

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Main Authors: Sandra Concepcion Das, Kavitha Varadharajan, Muralitharan Shanmugakonar, Hamda A. Al-Naemi
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-08-01
Series:Frontiers in Pharmacology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2021.663048/full
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author Sandra Concepcion Das
Sandra Concepcion Das
Kavitha Varadharajan
Muralitharan Shanmugakonar
Hamda A. Al-Naemi
Hamda A. Al-Naemi
author_facet Sandra Concepcion Das
Sandra Concepcion Das
Kavitha Varadharajan
Muralitharan Shanmugakonar
Hamda A. Al-Naemi
Hamda A. Al-Naemi
author_sort Sandra Concepcion Das
collection DOAJ
description The aim of this study was to evaluate the role of chronic cadmium exposure in modulating cardiac matrix metalloproteinases (MMPs) in the heart of rats. Adult male Sprague-Dawley rats were exposed to 15 ppm CdCl2 in drinking water for 10 weeks followed by withdrawal of cadmium treatment for 4 weeks. Following the completion of the treatment, gene expression of inflammatory mediators (IL-1β, IL-6, IL-10, TNF-α and NF-κB), protein expression of MMP-2, MMP-9 and their respective inhibitors- TIMP-1 and TIMP-2, and gelatinolytic activity of MMP-2 and MMP-9 were determined. At the protein level, cadmium incites a differential effect on the expression and activity of gelatinases and their endogenous inhibitors in an exposure-dependent manner. Results also show that the administered cadmium dose elicits an inflammatory response until week 10 that slightly diminishes after 4 weeks. This study provides evidence of cadmium-induced imbalance in the MMP-TIMP system in the cardiac tissue. This imbalance may be mediated by cadmium-induced inflammation that could contribute to various cardiovascular pathologies.
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spelling doaj.art-fa7bbf634cea44549a45e1963b53fba22022-12-21T22:50:46ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-08-011210.3389/fphar.2021.663048663048Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley RatSandra Concepcion Das0Sandra Concepcion Das1Kavitha Varadharajan2Muralitharan Shanmugakonar3Hamda A. Al-Naemi4Hamda A. Al-Naemi5Laboratory Animal Research Center, Qatar University, Doha, QatarDepartment of Biological and Environmental Sciences, Qatar University, Doha, QatarLaboratory Animal Research Center, Qatar University, Doha, QatarLaboratory Animal Research Center, Qatar University, Doha, QatarLaboratory Animal Research Center, Qatar University, Doha, QatarDepartment of Biological and Environmental Sciences, Qatar University, Doha, QatarThe aim of this study was to evaluate the role of chronic cadmium exposure in modulating cardiac matrix metalloproteinases (MMPs) in the heart of rats. Adult male Sprague-Dawley rats were exposed to 15 ppm CdCl2 in drinking water for 10 weeks followed by withdrawal of cadmium treatment for 4 weeks. Following the completion of the treatment, gene expression of inflammatory mediators (IL-1β, IL-6, IL-10, TNF-α and NF-κB), protein expression of MMP-2, MMP-9 and their respective inhibitors- TIMP-1 and TIMP-2, and gelatinolytic activity of MMP-2 and MMP-9 were determined. At the protein level, cadmium incites a differential effect on the expression and activity of gelatinases and their endogenous inhibitors in an exposure-dependent manner. Results also show that the administered cadmium dose elicits an inflammatory response until week 10 that slightly diminishes after 4 weeks. This study provides evidence of cadmium-induced imbalance in the MMP-TIMP system in the cardiac tissue. This imbalance may be mediated by cadmium-induced inflammation that could contribute to various cardiovascular pathologies.https://www.frontiersin.org/articles/10.3389/fphar.2021.663048/fullcadmiumcardiacinflammatory cytokinesMMPTIMP
spellingShingle Sandra Concepcion Das
Sandra Concepcion Das
Kavitha Varadharajan
Muralitharan Shanmugakonar
Hamda A. Al-Naemi
Hamda A. Al-Naemi
Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat
Frontiers in Pharmacology
cadmium
cardiac
inflammatory cytokines
MMP
TIMP
title Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat
title_full Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat
title_fullStr Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat
title_full_unstemmed Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat
title_short Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat
title_sort chronic cadmium exposure alters cardiac matrix metalloproteinases in the heart of sprague dawley rat
topic cadmium
cardiac
inflammatory cytokines
MMP
TIMP
url https://www.frontiersin.org/articles/10.3389/fphar.2021.663048/full
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