Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat
The aim of this study was to evaluate the role of chronic cadmium exposure in modulating cardiac matrix metalloproteinases (MMPs) in the heart of rats. Adult male Sprague-Dawley rats were exposed to 15 ppm CdCl2 in drinking water for 10 weeks followed by withdrawal of cadmium treatment for 4 weeks....
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Frontiers Media S.A.
2021-08-01
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Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.663048/full |
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author | Sandra Concepcion Das Sandra Concepcion Das Kavitha Varadharajan Muralitharan Shanmugakonar Hamda A. Al-Naemi Hamda A. Al-Naemi |
author_facet | Sandra Concepcion Das Sandra Concepcion Das Kavitha Varadharajan Muralitharan Shanmugakonar Hamda A. Al-Naemi Hamda A. Al-Naemi |
author_sort | Sandra Concepcion Das |
collection | DOAJ |
description | The aim of this study was to evaluate the role of chronic cadmium exposure in modulating cardiac matrix metalloproteinases (MMPs) in the heart of rats. Adult male Sprague-Dawley rats were exposed to 15 ppm CdCl2 in drinking water for 10 weeks followed by withdrawal of cadmium treatment for 4 weeks. Following the completion of the treatment, gene expression of inflammatory mediators (IL-1β, IL-6, IL-10, TNF-α and NF-κB), protein expression of MMP-2, MMP-9 and their respective inhibitors- TIMP-1 and TIMP-2, and gelatinolytic activity of MMP-2 and MMP-9 were determined. At the protein level, cadmium incites a differential effect on the expression and activity of gelatinases and their endogenous inhibitors in an exposure-dependent manner. Results also show that the administered cadmium dose elicits an inflammatory response until week 10 that slightly diminishes after 4 weeks. This study provides evidence of cadmium-induced imbalance in the MMP-TIMP system in the cardiac tissue. This imbalance may be mediated by cadmium-induced inflammation that could contribute to various cardiovascular pathologies. |
first_indexed | 2024-12-14T19:09:39Z |
format | Article |
id | doaj.art-fa7bbf634cea44549a45e1963b53fba2 |
institution | Directory Open Access Journal |
issn | 1663-9812 |
language | English |
last_indexed | 2024-12-14T19:09:39Z |
publishDate | 2021-08-01 |
publisher | Frontiers Media S.A. |
record_format | Article |
series | Frontiers in Pharmacology |
spelling | doaj.art-fa7bbf634cea44549a45e1963b53fba22022-12-21T22:50:46ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-08-011210.3389/fphar.2021.663048663048Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley RatSandra Concepcion Das0Sandra Concepcion Das1Kavitha Varadharajan2Muralitharan Shanmugakonar3Hamda A. Al-Naemi4Hamda A. Al-Naemi5Laboratory Animal Research Center, Qatar University, Doha, QatarDepartment of Biological and Environmental Sciences, Qatar University, Doha, QatarLaboratory Animal Research Center, Qatar University, Doha, QatarLaboratory Animal Research Center, Qatar University, Doha, QatarLaboratory Animal Research Center, Qatar University, Doha, QatarDepartment of Biological and Environmental Sciences, Qatar University, Doha, QatarThe aim of this study was to evaluate the role of chronic cadmium exposure in modulating cardiac matrix metalloproteinases (MMPs) in the heart of rats. Adult male Sprague-Dawley rats were exposed to 15 ppm CdCl2 in drinking water for 10 weeks followed by withdrawal of cadmium treatment for 4 weeks. Following the completion of the treatment, gene expression of inflammatory mediators (IL-1β, IL-6, IL-10, TNF-α and NF-κB), protein expression of MMP-2, MMP-9 and their respective inhibitors- TIMP-1 and TIMP-2, and gelatinolytic activity of MMP-2 and MMP-9 were determined. At the protein level, cadmium incites a differential effect on the expression and activity of gelatinases and their endogenous inhibitors in an exposure-dependent manner. Results also show that the administered cadmium dose elicits an inflammatory response until week 10 that slightly diminishes after 4 weeks. This study provides evidence of cadmium-induced imbalance in the MMP-TIMP system in the cardiac tissue. This imbalance may be mediated by cadmium-induced inflammation that could contribute to various cardiovascular pathologies.https://www.frontiersin.org/articles/10.3389/fphar.2021.663048/fullcadmiumcardiacinflammatory cytokinesMMPTIMP |
spellingShingle | Sandra Concepcion Das Sandra Concepcion Das Kavitha Varadharajan Muralitharan Shanmugakonar Hamda A. Al-Naemi Hamda A. Al-Naemi Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat Frontiers in Pharmacology cadmium cardiac inflammatory cytokines MMP TIMP |
title | Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat |
title_full | Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat |
title_fullStr | Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat |
title_full_unstemmed | Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat |
title_short | Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat |
title_sort | chronic cadmium exposure alters cardiac matrix metalloproteinases in the heart of sprague dawley rat |
topic | cadmium cardiac inflammatory cytokines MMP TIMP |
url | https://www.frontiersin.org/articles/10.3389/fphar.2021.663048/full |
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