Identification of a novel monocytic phenotype in Classic Hodgkin Lymphoma tumor microenvironment.
Classic Hodgkin lymphoma (CHL) characteristically shows few malignant cells in a microenvironment comprised of mixed inflammatory cells. Although CHL is associated with a high cure rate, recent studies have associated poor prognosis with absolute monocyte count in peripheral blood and increased mono...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0224621 |
| _version_ | 1819029164502548480 |
|---|---|
| author | Ginell R Post Youzhong Yuan Emily R Holthoff Charles M Quick Steven R Post |
| author_facet | Ginell R Post Youzhong Yuan Emily R Holthoff Charles M Quick Steven R Post |
| author_sort | Ginell R Post |
| collection | DOAJ |
| description | Classic Hodgkin lymphoma (CHL) characteristically shows few malignant cells in a microenvironment comprised of mixed inflammatory cells. Although CHL is associated with a high cure rate, recent studies have associated poor prognosis with absolute monocyte count in peripheral blood and increased monocyte/macrophages in involved lymph nodes. Thus, the role of monocytic infiltration and macrophage differentiation in the tumor microenvironment of CHL may be more relevant than absolute macrophage numbers to defining prognosis in CHL patients and potentially have therapeutic implications. Most studies identify tumor-associated macrophages (TAMs) using markers (e.g., CD68) expressed by macrophages and other mononuclear phagocytes, such as monocytes. In contrast, Class A Scavenger Receptor (SR-A/CD204) is expressed by tissue macrophages but not monocytic precursors. In this study, we examined SR-A expression in CHL (n = 43), and compared its expression with that of other macrophage markers. We confirmed a high prevalence of mononuclear cells that stained with CD68, CD163, and CD14 in CHL lymph nodes. However, SR-A protein expression determined by immunohistochemistry was limited to macrophages localized in sclerotic bands characteristic of nodular sclerosis CHL. In contrast, SR-A protein was readily detectable in lymph nodes with metastatic tumor, extra-nodal CHL, T cell/histiocyte-rich large B cell lymphoma, and resident macrophages in non-malignant tissues, including spleen, lymph node, liver and lung. The results of SR-A protein expression paralleled the expression of SR-A mRNA determined by quantitative RT-PCR. These data provide evidence that tumor-infiltrating monocyte/macrophages in CHL have a unique phenotype that likely depends on the microenvironment of nodal CHL. |
| first_indexed | 2024-12-21T06:09:54Z |
| format | Article |
| id | doaj.art-fa7bc9b658854104be253680ab20d362 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T06:09:54Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fa7bc9b658854104be253680ab20d3622022-12-21T19:13:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011411e022462110.1371/journal.pone.0224621Identification of a novel monocytic phenotype in Classic Hodgkin Lymphoma tumor microenvironment.Ginell R PostYouzhong YuanEmily R HolthoffCharles M QuickSteven R PostClassic Hodgkin lymphoma (CHL) characteristically shows few malignant cells in a microenvironment comprised of mixed inflammatory cells. Although CHL is associated with a high cure rate, recent studies have associated poor prognosis with absolute monocyte count in peripheral blood and increased monocyte/macrophages in involved lymph nodes. Thus, the role of monocytic infiltration and macrophage differentiation in the tumor microenvironment of CHL may be more relevant than absolute macrophage numbers to defining prognosis in CHL patients and potentially have therapeutic implications. Most studies identify tumor-associated macrophages (TAMs) using markers (e.g., CD68) expressed by macrophages and other mononuclear phagocytes, such as monocytes. In contrast, Class A Scavenger Receptor (SR-A/CD204) is expressed by tissue macrophages but not monocytic precursors. In this study, we examined SR-A expression in CHL (n = 43), and compared its expression with that of other macrophage markers. We confirmed a high prevalence of mononuclear cells that stained with CD68, CD163, and CD14 in CHL lymph nodes. However, SR-A protein expression determined by immunohistochemistry was limited to macrophages localized in sclerotic bands characteristic of nodular sclerosis CHL. In contrast, SR-A protein was readily detectable in lymph nodes with metastatic tumor, extra-nodal CHL, T cell/histiocyte-rich large B cell lymphoma, and resident macrophages in non-malignant tissues, including spleen, lymph node, liver and lung. The results of SR-A protein expression paralleled the expression of SR-A mRNA determined by quantitative RT-PCR. These data provide evidence that tumor-infiltrating monocyte/macrophages in CHL have a unique phenotype that likely depends on the microenvironment of nodal CHL.https://doi.org/10.1371/journal.pone.0224621 |
| spellingShingle | Ginell R Post Youzhong Yuan Emily R Holthoff Charles M Quick Steven R Post Identification of a novel monocytic phenotype in Classic Hodgkin Lymphoma tumor microenvironment. PLoS ONE |
| title | Identification of a novel monocytic phenotype in Classic Hodgkin Lymphoma tumor microenvironment. |
| title_full | Identification of a novel monocytic phenotype in Classic Hodgkin Lymphoma tumor microenvironment. |
| title_fullStr | Identification of a novel monocytic phenotype in Classic Hodgkin Lymphoma tumor microenvironment. |
| title_full_unstemmed | Identification of a novel monocytic phenotype in Classic Hodgkin Lymphoma tumor microenvironment. |
| title_short | Identification of a novel monocytic phenotype in Classic Hodgkin Lymphoma tumor microenvironment. |
| title_sort | identification of a novel monocytic phenotype in classic hodgkin lymphoma tumor microenvironment |
| url | https://doi.org/10.1371/journal.pone.0224621 |
| work_keys_str_mv | AT ginellrpost identificationofanovelmonocyticphenotypeinclassichodgkinlymphomatumormicroenvironment AT youzhongyuan identificationofanovelmonocyticphenotypeinclassichodgkinlymphomatumormicroenvironment AT emilyrholthoff identificationofanovelmonocyticphenotypeinclassichodgkinlymphomatumormicroenvironment AT charlesmquick identificationofanovelmonocyticphenotypeinclassichodgkinlymphomatumormicroenvironment AT stevenrpost identificationofanovelmonocyticphenotypeinclassichodgkinlymphomatumormicroenvironment |