A multi-centre study on the role of the thioredoxin system in breast cancer cell proliferation
Redox proteins (thioredoxin, glutaredoxin) are key macromolecules capable of modulating intracellular processes. This determines research choices in the field of redox-dependent cell proliferation management. The study of the molecular mechanisms of the onset, development and progression of malignan...
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2018-12-01
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| Series: | Бюллетень сибирской медицины |
| Subjects: | |
| Online Access: | https://bulletin.ssmu.ru/jour/article/view/1991 |
| _version_ | 1797872867715579904 |
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| author | E. V. Shakhristova E. A. Stepovaya O. L. Nosareva L. S. Litvinova D. A. Skuratovskaya E. V. Rudikov A. A. Sadykova V. V. Novitsky |
| author_facet | E. V. Shakhristova E. A. Stepovaya O. L. Nosareva L. S. Litvinova D. A. Skuratovskaya E. V. Rudikov A. A. Sadykova V. V. Novitsky |
| author_sort | E. V. Shakhristova |
| collection | DOAJ |
| description | Redox proteins (thioredoxin, glutaredoxin) are key macromolecules capable of modulating intracellular processes. This determines research choices in the field of redox-dependent cell proliferation management. The study of the molecular mechanisms of the onset, development and progression of malignant neoplasms underlies the search for tumor-associated markers and potential targets for personalized antitumor therapy.Purpose. To establish the role of the “thioredoxin – thioredoxin-reductase” system in the impaired proliferation of mammary adenocarcinoma cells under the action of the cyclin-dependent protein kinase roskovitin blocker.Materials and methods. The study was carried out using the culture of mammary adenocarcinoma cells of the MCF-7 line incubated in the presence and absence of roskovitin at a final concentration of 20 μM for 18 h. The intracellular content of thioredoxin and protein regulators of proliferation (cyclin E and cyclin-dependent protein kinase 2) were determined by Western blotting technique, the expression level of thioredoxin mRNA was determined by real-time polymerase chain reaction and the activity of thioredoxin-reductase was measured by a spectrophotometric method.Results. It was established that the decrease in proliferative activity of MCF-7 tumor cells incubated in the presence of roskovitin was accompanied by a decrease in the content of cyclin E and cyclin-dependent kinase on the background of a decrease in the expression level of thioredoxin mRNA and an increase in the activity of thioredoxin-reductase.Conclusion. The involvement of the components of the thioredoxin system (thioredoxin, thioredoxinreductase) in disrupting the proliferation of MCF-7 tumor cells was detected under the action of the cyclindependent protein kinases of roskovitin. |
| first_indexed | 2024-04-10T01:07:01Z |
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| id | doaj.art-fa7bf85606374c2ca112d148581ffe64 |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:07:01Z |
| publishDate | 2018-12-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-fa7bf85606374c2ca112d148581ffe642023-03-13T09:58:24ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842018-12-0117418018610.20538/1682-0363-2018-4-180-1861464A multi-centre study on the role of the thioredoxin system in breast cancer cell proliferationE. V. Shakhristova0E. A. Stepovaya1O. L. Nosareva2L. S. Litvinova3D. A. Skuratovskaya4E. V. Rudikov5A. A. Sadykova6V. V. Novitsky7Сибирский государственный медицинский университет (СибГМУ)Сибирский государственный медицинский университет (СибГМУ)Сибирский государственный медицинский университет (СибГМУ)Балтийский федеральный университет имени Иммануила Канта (БФУ им. И. Канта)Балтийский федеральный университет имени Иммануила Канта (БФУ им. И. Канта)Сибирский государственный медицинский университет (СибГМУ)Сибирский государственный медицинский университет (СибГМУ)Сибирский государственный медицинский университет (СибГМУ)Redox proteins (thioredoxin, glutaredoxin) are key macromolecules capable of modulating intracellular processes. This determines research choices in the field of redox-dependent cell proliferation management. The study of the molecular mechanisms of the onset, development and progression of malignant neoplasms underlies the search for tumor-associated markers and potential targets for personalized antitumor therapy.Purpose. To establish the role of the “thioredoxin – thioredoxin-reductase” system in the impaired proliferation of mammary adenocarcinoma cells under the action of the cyclin-dependent protein kinase roskovitin blocker.Materials and methods. The study was carried out using the culture of mammary adenocarcinoma cells of the MCF-7 line incubated in the presence and absence of roskovitin at a final concentration of 20 μM for 18 h. The intracellular content of thioredoxin and protein regulators of proliferation (cyclin E and cyclin-dependent protein kinase 2) were determined by Western blotting technique, the expression level of thioredoxin mRNA was determined by real-time polymerase chain reaction and the activity of thioredoxin-reductase was measured by a spectrophotometric method.Results. It was established that the decrease in proliferative activity of MCF-7 tumor cells incubated in the presence of roskovitin was accompanied by a decrease in the content of cyclin E and cyclin-dependent kinase on the background of a decrease in the expression level of thioredoxin mRNA and an increase in the activity of thioredoxin-reductase.Conclusion. The involvement of the components of the thioredoxin system (thioredoxin, thioredoxinreductase) in disrupting the proliferation of MCF-7 tumor cells was detected under the action of the cyclindependent protein kinases of roskovitin.https://bulletin.ssmu.ru/jour/article/view/1991тиоредоксинаденокарцинома молочной железыредокс-регуляцияпролиферация |
| spellingShingle | E. V. Shakhristova E. A. Stepovaya O. L. Nosareva L. S. Litvinova D. A. Skuratovskaya E. V. Rudikov A. A. Sadykova V. V. Novitsky A multi-centre study on the role of the thioredoxin system in breast cancer cell proliferation Бюллетень сибирской медицины тиоредоксин аденокарцинома молочной железы редокс-регуляция пролиферация |
| title | A multi-centre study on the role of the thioredoxin system in breast cancer cell proliferation |
| title_full | A multi-centre study on the role of the thioredoxin system in breast cancer cell proliferation |
| title_fullStr | A multi-centre study on the role of the thioredoxin system in breast cancer cell proliferation |
| title_full_unstemmed | A multi-centre study on the role of the thioredoxin system in breast cancer cell proliferation |
| title_short | A multi-centre study on the role of the thioredoxin system in breast cancer cell proliferation |
| title_sort | multi centre study on the role of the thioredoxin system in breast cancer cell proliferation |
| topic | тиоредоксин аденокарцинома молочной железы редокс-регуляция пролиферация |
| url | https://bulletin.ssmu.ru/jour/article/view/1991 |
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