Protective effects of Aureobasidium pullulans lysate on UV-damaged human skin fibroblasts and HaCaT cells
Abstract Background Aureobasidium pullulans (A. pullulans) has a wide range of applications. Ultraviolet (UV) rays from the sun can cause skin photoaging. In order to explore the protective effect and application potential of A. pullulans lysate on UV-damaged human skin fibroblasts (HSF) and HaCaT C...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2023-08-01
|
| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40643-023-00678-9 |
| _version_ | 1797578499831103488 |
|---|---|
| author | Xin Wang Yongtao Zhang Dongdong Wang Ning Su Li Yang Hao Fu Jiachan Zhang Meng Li Changtao Wang |
| author_facet | Xin Wang Yongtao Zhang Dongdong Wang Ning Su Li Yang Hao Fu Jiachan Zhang Meng Li Changtao Wang |
| author_sort | Xin Wang |
| collection | DOAJ |
| description | Abstract Background Aureobasidium pullulans (A. pullulans) has a wide range of applications. Ultraviolet (UV) rays from the sun can cause skin photoaging. In order to explore the protective effect and application potential of A. pullulans lysate on UV-damaged human skin fibroblasts (HSF) and HaCaT Cells, this study investigates the anti-aging and anti-inflammatory effects of A. pullulans lysate as well as the mechanism of anti-oxidative stress at the cellular and molecular levels through cytotoxicity experiments, enzyme-linked immunosorbent assays (ELISA), and real-time quantitative PCR (RT-qPCR). Results The experimental results have shown that the A. pullulans lysate can effectively reduce the loss of extracellular matrix components (EMC), such as collagen and hyaluronic acid (HA). It is also capable of scavenging excess reactive oxygen species (ROS) from the body, thereby increasing the activity of catalase, decreasing the overexpression of intracellular matrix metalloproteinases (MMPs), enhancing the gene expression of metalloproteinase inhibitors (TIMPs), and decreasing the level of inflammatory factors, reducing UV-induced apoptosis of HaCaT cells. Meanwhile, oxidative stress homeostasis is also regulated through the Nrf2/Keap1 and MAPK signaling pathways. Conclusions This study shows that the A. pullulans lysate has the potential to resist photoaging. Graphical Abstract |
| first_indexed | 2024-03-10T22:23:45Z |
| format | Article |
| id | doaj.art-fa7f2f0bc34d4b67ac1ecb93e49ae846 |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-03-10T22:23:45Z |
| publishDate | 2023-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-fa7f2f0bc34d4b67ac1ecb93e49ae8462023-11-19T12:12:37ZengSpringerOpenBioresources and Bioprocessing2197-43652023-08-0110111410.1186/s40643-023-00678-9Protective effects of Aureobasidium pullulans lysate on UV-damaged human skin fibroblasts and HaCaT cellsXin Wang0Yongtao Zhang1Dongdong Wang2Ning Su3Li Yang4Hao Fu5Jiachan Zhang6Meng Li7Changtao Wang8Beijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business UniversityBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business UniversityBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business UniversityChinese Academy of Inspection and QuarantineBeijing Sino-German Union Cosmetic Institute Co., LtdBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business UniversityBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business UniversityBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business UniversityBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business UniversityAbstract Background Aureobasidium pullulans (A. pullulans) has a wide range of applications. Ultraviolet (UV) rays from the sun can cause skin photoaging. In order to explore the protective effect and application potential of A. pullulans lysate on UV-damaged human skin fibroblasts (HSF) and HaCaT Cells, this study investigates the anti-aging and anti-inflammatory effects of A. pullulans lysate as well as the mechanism of anti-oxidative stress at the cellular and molecular levels through cytotoxicity experiments, enzyme-linked immunosorbent assays (ELISA), and real-time quantitative PCR (RT-qPCR). Results The experimental results have shown that the A. pullulans lysate can effectively reduce the loss of extracellular matrix components (EMC), such as collagen and hyaluronic acid (HA). It is also capable of scavenging excess reactive oxygen species (ROS) from the body, thereby increasing the activity of catalase, decreasing the overexpression of intracellular matrix metalloproteinases (MMPs), enhancing the gene expression of metalloproteinase inhibitors (TIMPs), and decreasing the level of inflammatory factors, reducing UV-induced apoptosis of HaCaT cells. Meanwhile, oxidative stress homeostasis is also regulated through the Nrf2/Keap1 and MAPK signaling pathways. Conclusions This study shows that the A. pullulans lysate has the potential to resist photoaging. Graphical Abstracthttps://doi.org/10.1186/s40643-023-00678-9A. pullulans lysateUVHuman skin fibroblastsHuman immortalized keratinocytes |
| spellingShingle | Xin Wang Yongtao Zhang Dongdong Wang Ning Su Li Yang Hao Fu Jiachan Zhang Meng Li Changtao Wang Protective effects of Aureobasidium pullulans lysate on UV-damaged human skin fibroblasts and HaCaT cells Bioresources and Bioprocessing A. pullulans lysate UV Human skin fibroblasts Human immortalized keratinocytes |
| title | Protective effects of Aureobasidium pullulans lysate on UV-damaged human skin fibroblasts and HaCaT cells |
| title_full | Protective effects of Aureobasidium pullulans lysate on UV-damaged human skin fibroblasts and HaCaT cells |
| title_fullStr | Protective effects of Aureobasidium pullulans lysate on UV-damaged human skin fibroblasts and HaCaT cells |
| title_full_unstemmed | Protective effects of Aureobasidium pullulans lysate on UV-damaged human skin fibroblasts and HaCaT cells |
| title_short | Protective effects of Aureobasidium pullulans lysate on UV-damaged human skin fibroblasts and HaCaT cells |
| title_sort | protective effects of aureobasidium pullulans lysate on uv damaged human skin fibroblasts and hacat cells |
| topic | A. pullulans lysate UV Human skin fibroblasts Human immortalized keratinocytes |
| url | https://doi.org/10.1186/s40643-023-00678-9 |
| work_keys_str_mv | AT xinwang protectiveeffectsofaureobasidiumpullulanslysateonuvdamagedhumanskinfibroblastsandhacatcells AT yongtaozhang protectiveeffectsofaureobasidiumpullulanslysateonuvdamagedhumanskinfibroblastsandhacatcells AT dongdongwang protectiveeffectsofaureobasidiumpullulanslysateonuvdamagedhumanskinfibroblastsandhacatcells AT ningsu protectiveeffectsofaureobasidiumpullulanslysateonuvdamagedhumanskinfibroblastsandhacatcells AT liyang protectiveeffectsofaureobasidiumpullulanslysateonuvdamagedhumanskinfibroblastsandhacatcells AT haofu protectiveeffectsofaureobasidiumpullulanslysateonuvdamagedhumanskinfibroblastsandhacatcells AT jiachanzhang protectiveeffectsofaureobasidiumpullulanslysateonuvdamagedhumanskinfibroblastsandhacatcells AT mengli protectiveeffectsofaureobasidiumpullulanslysateonuvdamagedhumanskinfibroblastsandhacatcells AT changtaowang protectiveeffectsofaureobasidiumpullulanslysateonuvdamagedhumanskinfibroblastsandhacatcells |