CdsH Contributes to the Replication of <i>Salmonella</i> Typhimurium inside Epithelial Cells in a Cysteine-Supplemented Medium
<i>Salmonella</i> Typhimurium is a facultative, intracellular pathogen whose products range from self-limited gastroenteritis to systemic diseases. Food ingestion increases biomolecules’ concentration in the intestinal lumen, including amino acids such as cysteine, which is toxic in a co...
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MDPI AG
2020-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/12/2019 |
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| author | Fernando Díaz-Yáñez Ricardo Álvarez Iván L. Calderón Juan A. Fuentes Fernando Gil |
| author_facet | Fernando Díaz-Yáñez Ricardo Álvarez Iván L. Calderón Juan A. Fuentes Fernando Gil |
| author_sort | Fernando Díaz-Yáñez |
| collection | DOAJ |
| description | <i>Salmonella</i> Typhimurium is a facultative, intracellular pathogen whose products range from self-limited gastroenteritis to systemic diseases. Food ingestion increases biomolecules’ concentration in the intestinal lumen, including amino acids such as cysteine, which is toxic in a concentration-dependent manner. When cysteine’s intracellular concentration reaches toxic levels, <i>S</i>. Typhimurium expresses a cysteine-inducible enzyme (CdsH), which converts cysteine into pyruvate, sulfide, and ammonia. Despite this evidence, the biological context of <i>cdsH</i>’s role is not completely clear, especially in the infective cycle. Since inside epithelial cells both <i>cdsH</i> and its positive regulator, <i>ybaO</i>, are overexpressed, we hypothesized a possible role of <i>cdsH</i> in the intestinal phase of the infection. To test this hypothesis, we used an in vitro model of HT-29 cell infection, adding extra cysteine to the culture medium during the infective process. We observed that, at 6 h post-invasion, the wild type <i>S.</i> Typhimurium proliferated 30% more than the Δ<i>cdsH</i> strain in the presence of extra cysteine. This result shows that <i>cdsH</i> contributes to the bacterial replication in the intracellular environment in increased concentrations of extracellular cysteine, strongly suggesting that <i>cdsH</i> participates by increasing the bacterial fitness in the intestinal phase of the <i>S.</i> Typhimurium infection. |
| first_indexed | 2024-03-10T14:00:38Z |
| format | Article |
| id | doaj.art-fa815a6c273c44298c5e43599f69aa83 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:00:38Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-fa815a6c273c44298c5e43599f69aa832023-11-21T01:12:08ZengMDPI AGMicroorganisms2076-26072020-12-01812201910.3390/microorganisms8122019CdsH Contributes to the Replication of <i>Salmonella</i> Typhimurium inside Epithelial Cells in a Cysteine-Supplemented MediumFernando Díaz-Yáñez0Ricardo Álvarez1Iván L. Calderón2Juan A. Fuentes3Fernando Gil4Microbiota-Host Interactions and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, 8370186 Santiago, ChileMicrobiota-Host Interactions and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, 8370186 Santiago, ChileLaboratorio de RNAs Bacterianos, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, 8370186 Santiago, ChileLaboratorio de Genética y Patogénesis Bacteriana, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, 8370186 Santiago, ChileMicrobiota-Host Interactions and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, 8370186 Santiago, Chile<i>Salmonella</i> Typhimurium is a facultative, intracellular pathogen whose products range from self-limited gastroenteritis to systemic diseases. Food ingestion increases biomolecules’ concentration in the intestinal lumen, including amino acids such as cysteine, which is toxic in a concentration-dependent manner. When cysteine’s intracellular concentration reaches toxic levels, <i>S</i>. Typhimurium expresses a cysteine-inducible enzyme (CdsH), which converts cysteine into pyruvate, sulfide, and ammonia. Despite this evidence, the biological context of <i>cdsH</i>’s role is not completely clear, especially in the infective cycle. Since inside epithelial cells both <i>cdsH</i> and its positive regulator, <i>ybaO</i>, are overexpressed, we hypothesized a possible role of <i>cdsH</i> in the intestinal phase of the infection. To test this hypothesis, we used an in vitro model of HT-29 cell infection, adding extra cysteine to the culture medium during the infective process. We observed that, at 6 h post-invasion, the wild type <i>S.</i> Typhimurium proliferated 30% more than the Δ<i>cdsH</i> strain in the presence of extra cysteine. This result shows that <i>cdsH</i> contributes to the bacterial replication in the intracellular environment in increased concentrations of extracellular cysteine, strongly suggesting that <i>cdsH</i> participates by increasing the bacterial fitness in the intestinal phase of the <i>S.</i> Typhimurium infection.https://www.mdpi.com/2076-2607/8/12/2019<i>cdsH</i> expressioncysteine toxicityepithelial cell infection |
| spellingShingle | Fernando Díaz-Yáñez Ricardo Álvarez Iván L. Calderón Juan A. Fuentes Fernando Gil CdsH Contributes to the Replication of <i>Salmonella</i> Typhimurium inside Epithelial Cells in a Cysteine-Supplemented Medium Microorganisms <i>cdsH</i> expression cysteine toxicity epithelial cell infection |
| title | CdsH Contributes to the Replication of <i>Salmonella</i> Typhimurium inside Epithelial Cells in a Cysteine-Supplemented Medium |
| title_full | CdsH Contributes to the Replication of <i>Salmonella</i> Typhimurium inside Epithelial Cells in a Cysteine-Supplemented Medium |
| title_fullStr | CdsH Contributes to the Replication of <i>Salmonella</i> Typhimurium inside Epithelial Cells in a Cysteine-Supplemented Medium |
| title_full_unstemmed | CdsH Contributes to the Replication of <i>Salmonella</i> Typhimurium inside Epithelial Cells in a Cysteine-Supplemented Medium |
| title_short | CdsH Contributes to the Replication of <i>Salmonella</i> Typhimurium inside Epithelial Cells in a Cysteine-Supplemented Medium |
| title_sort | cdsh contributes to the replication of i salmonella i typhimurium inside epithelial cells in a cysteine supplemented medium |
| topic | <i>cdsH</i> expression cysteine toxicity epithelial cell infection |
| url | https://www.mdpi.com/2076-2607/8/12/2019 |
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