Detection of Rotavirus from diarrhoeic cow calves in Mathura, India

Diarrhoeal diseases are important cause of morbidity and mortality in neonates of various animal species. Rotaviruses cause neonatal diarrhea in calves. The present study was carried out to determine the prevalence of rotavirus infection among cow calves in Mathura and adjacent regions. During the p...

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Main Authors: Krishna Kumar, A. Tewari, S. K. Dash, A. Goel, A. K. Bhatia
Format: Article
Language:English
Published: Veterinary World 2011-01-01
Series:Veterinary World
Subjects:
Online Access:http://www.scopemed.org/mnstemps/2/2-1319388079.pdf
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author Krishna Kumar
A. Tewari
S. K. Dash
A. Goel
A. K. Bhatia
author_facet Krishna Kumar
A. Tewari
S. K. Dash
A. Goel
A. K. Bhatia
author_sort Krishna Kumar
collection DOAJ
description Diarrhoeal diseases are important cause of morbidity and mortality in neonates of various animal species. Rotaviruses cause neonatal diarrhea in calves. The present study was carried out to determine the prevalence of rotavirus infection among cow calves in Mathura and adjacent regions. During the present study 101 diarrheic and 29 non diarrheic stool samples collected from cow calves were screened for rotavirus. Of the101 diarrheic samples 17 samples (16.83%) were found to be positive for rotavirus by RNA PAGE. All the non-diarrheic samples were negative for rotavirus. All the isolates exhibited 4-2-3-2 migration pattern suggesting group A rotavirus. Depending on migration of 10th and 11th segments, all the isolates were of long pattern. Three different electropherotypes were detected in this study period. Male diarrheic calves were found to be more susceptible to rotavirus infection (20.37%) than female diarrheic calves (12.76%). Besides Rotavirus antigen was detected by ELISA. [Vet. World 2011; 4(12.000): 554-556]
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spelling doaj.art-fa83f3bc387c4a4ea849acaa5714c95e2022-12-21T16:42:59ZengVeterinary WorldVeterinary World0972-89882231-09162011-01-01412.000554556Detection of Rotavirus from diarrhoeic cow calves in Mathura, IndiaKrishna KumarA. TewariS. K. DashA. GoelA. K. BhatiaDiarrhoeal diseases are important cause of morbidity and mortality in neonates of various animal species. Rotaviruses cause neonatal diarrhea in calves. The present study was carried out to determine the prevalence of rotavirus infection among cow calves in Mathura and adjacent regions. During the present study 101 diarrheic and 29 non diarrheic stool samples collected from cow calves were screened for rotavirus. Of the101 diarrheic samples 17 samples (16.83%) were found to be positive for rotavirus by RNA PAGE. All the non-diarrheic samples were negative for rotavirus. All the isolates exhibited 4-2-3-2 migration pattern suggesting group A rotavirus. Depending on migration of 10th and 11th segments, all the isolates were of long pattern. Three different electropherotypes were detected in this study period. Male diarrheic calves were found to be more susceptible to rotavirus infection (20.37%) than female diarrheic calves (12.76%). Besides Rotavirus antigen was detected by ELISA. [Vet. World 2011; 4(12.000): 554-556]http://www.scopemed.org/mnstemps/2/2-1319388079.pdfBovine rotavirusDiarrhoeaRNA PAGEELISAVirusElectropherotypes
spellingShingle Krishna Kumar
A. Tewari
S. K. Dash
A. Goel
A. K. Bhatia
Detection of Rotavirus from diarrhoeic cow calves in Mathura, India
Veterinary World
Bovine rotavirus
Diarrhoea
RNA PAGE
ELISA
Virus
Electropherotypes
title Detection of Rotavirus from diarrhoeic cow calves in Mathura, India
title_full Detection of Rotavirus from diarrhoeic cow calves in Mathura, India
title_fullStr Detection of Rotavirus from diarrhoeic cow calves in Mathura, India
title_full_unstemmed Detection of Rotavirus from diarrhoeic cow calves in Mathura, India
title_short Detection of Rotavirus from diarrhoeic cow calves in Mathura, India
title_sort detection of rotavirus from diarrhoeic cow calves in mathura india
topic Bovine rotavirus
Diarrhoea
RNA PAGE
ELISA
Virus
Electropherotypes
url http://www.scopemed.org/mnstemps/2/2-1319388079.pdf
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