Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets
Vitamin B2 (riboflavin) is a water-soluble vitamin that has important roles in human health. In this study, we developed a sensitive and specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and lateral-flow immunochromatographic assay (ICA) strip for t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2017.1360257 |
| _version_ | 1818341913185484800 |
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| author | Lu Zeng Wei Jiang Liqiang Liu Shanshan Song Hua Kuang |
| author_facet | Lu Zeng Wei Jiang Liqiang Liu Shanshan Song Hua Kuang |
| author_sort | Lu Zeng |
| collection | DOAJ |
| description | Vitamin B2 (riboflavin) is a water-soluble vitamin that has important roles in human health. In this study, we developed a sensitive and specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and lateral-flow immunochromatographic assay (ICA) strip for the rapid detection of vitamin B2. Following routine fusion and selection, the optimum monoclonal antibody against vitamin B2 was obtained. The 50% inhibitory concentration and limit of detection of ic-ELISA were 8.18 and 1.80 ng/mL, respectively. The cut-off value of the lateral-flow ICA strip was 50 ng/mL. The results revealed that our developed methods are suitable for the on-site detection and mass screening of vitamin B2 in food and pharmaceutical products. |
| first_indexed | 2024-12-13T16:06:21Z |
| format | Article |
| id | doaj.art-fa8474e991374c299caaae7d0aefa762 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-13T16:06:21Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-fa8474e991374c299caaae7d0aefa7622022-12-21T23:39:02ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-0129112113210.1080/09540105.2017.13602571360257Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tabletsLu Zeng0Wei Jiang1Liqiang Liu2Shanshan Song3Hua Kuang4State Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityVitamin B2 (riboflavin) is a water-soluble vitamin that has important roles in human health. In this study, we developed a sensitive and specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and lateral-flow immunochromatographic assay (ICA) strip for the rapid detection of vitamin B2. Following routine fusion and selection, the optimum monoclonal antibody against vitamin B2 was obtained. The 50% inhibitory concentration and limit of detection of ic-ELISA were 8.18 and 1.80 ng/mL, respectively. The cut-off value of the lateral-flow ICA strip was 50 ng/mL. The results revealed that our developed methods are suitable for the on-site detection and mass screening of vitamin B2 in food and pharmaceutical products.http://dx.doi.org/10.1080/09540105.2017.1360257vitamin b2monoclonal antibodyic-elisalateral-flow immunochromatographic assay strip |
| spellingShingle | Lu Zeng Wei Jiang Liqiang Liu Shanshan Song Hua Kuang Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets Food and Agricultural Immunology vitamin b2 monoclonal antibody ic-elisa lateral-flow immunochromatographic assay strip |
| title | Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets |
| title_full | Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets |
| title_fullStr | Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets |
| title_full_unstemmed | Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets |
| title_short | Development of ic-ELISA and lateral-flow immunochromatographic strip for detection of vitamin B2 in an energy drink and vitamin tablets |
| title_sort | development of ic elisa and lateral flow immunochromatographic strip for detection of vitamin b2 in an energy drink and vitamin tablets |
| topic | vitamin b2 monoclonal antibody ic-elisa lateral-flow immunochromatographic assay strip |
| url | http://dx.doi.org/10.1080/09540105.2017.1360257 |
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