Targeted Toxin-Based Selectable Drug-Free Enrichment of Mammalian Cells with High Transgene Expression

Targeted Toxin-Based Selectable Drug-Free Enrichment of Mammalian Cells with High Transgene Expression

Almost all transfection protocols for mammalian cells use a drug resistance gene for the selection of transfected cells. However, it always requires the characterization of each isolated clone regarding transgene expression, which is time-consuming and labor-intensive. In the current study, we devel...

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Bibliographic Details
Main Authors:	Satoshi Watanabe, Takayuki Sakurai, Shingo Nakamura, Masato Ohtsuka, Issei Saitoh, Eri Akasaka, Masahiro Sato
Format:	Article
Language:	English
Published:	MDPI AG 2013-02-01
Series:	Biology
Subjects:	a-Gal epitope BS-I-B4 lectin endo-b-galactosidase C porcine embryonic fibroblasts saporin targeted toxin high transgene expression
Online Access:	http://www.mdpi.com/2079-7737/2/1/341

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