Vardenafil Reduces Testicular Damage Following Ischemia/Reperfusion Injury in Rats
We investigated the effect of intraperitoneal vardenafil (1 mg/kg) administration during an ischemic period in a rat model of testicular torsion/detorsion (T/D). Twenty-one adult Wistar rats were equally randomized into a control group, a T/D group and a vardenafil group. The control group was desig...
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| Format: | Article |
| Language: | English |
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Wiley
2009-07-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1607551X09705303 |
| _version_ | 1811199727565275136 |
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| author | Bulent Erol Husnu Tokgoz Volkan Hanci Sibel Bektas Bulent Akduman Faruk Yencilek Gorkem Mungan Aydin Mungan |
| author_facet | Bulent Erol Husnu Tokgoz Volkan Hanci Sibel Bektas Bulent Akduman Faruk Yencilek Gorkem Mungan Aydin Mungan |
| author_sort | Bulent Erol |
| collection | DOAJ |
| description | We investigated the effect of intraperitoneal vardenafil (1 mg/kg) administration during an ischemic period in a rat model of testicular torsion/detorsion (T/D). Twenty-one adult Wistar rats were equally randomized into a control group, a T/D group and a vardenafil group. The control group was designed to collect basal values for biochemical and histopathological parameters. The T/D group underwent testicular torsion for 1 hour. The vardenafil group received vardenafil (1mg/kg) intraperitoneally at 30 minutes after torsion. All rats were sacrificed 4 hours after reperfusion to evaluate the tissue levels of malondialdehyde and total antioxidant status. Germ cell apoptosis was evaluated using the apoptosis protease activating factor 1 antibody in all groups. The expressions of endothelial nitric oxide synthase (NOS) and inducible NOS were also assessed in both testes of all rats. The malondialdehyde levels in the T/D group were significantly higher than in the control and vardenafil groups. There were also significant decreases in total antioxidant status in the T/D group compared with the control and vardenafil groups. Vardenafil treatment significantly reduced apoptosis protease activating factor 1, endothelial NOS and inducible NOS levels in the vardenafil group compared with the T/D group. Administration of 1 mg/kg vardenafil during testicular torsion decreased ischemia/reperfusion cellular damage. Our results indicate that the reduction in oxidative stress by vardenafil may play a major role in its cytoprotective effects. |
| first_indexed | 2024-04-12T01:53:17Z |
| format | Article |
| id | doaj.art-fa859b9ddfb0412aa459d16f5cdadb7f |
| institution | Directory Open Access Journal |
| issn | 1607-551X |
| language | English |
| last_indexed | 2024-04-12T01:53:17Z |
| publishDate | 2009-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-fa859b9ddfb0412aa459d16f5cdadb7f2022-12-22T03:52:53ZengWileyKaohsiung Journal of Medical Sciences1607-551X2009-07-0125737438010.1016/S1607-551X(09)70530-3Vardenafil Reduces Testicular Damage Following Ischemia/Reperfusion Injury in RatsBulent Erol0Husnu Tokgoz1Volkan Hanci2Sibel Bektas3Bulent Akduman4Faruk Yencilek5Gorkem Mungan6Aydin Mungan7Department of Urology, Zonguldak Karaelmas University, Zonguldak, TurkeyDepartment of Urology, Zonguldak Karaelmas University, Zonguldak, TurkeyDepartment of Anesthesiology, Zonguldak Karaelmas University, Zonguldak, TurkeyDepartment of Pathology, Zonguldak Karaelmas University, Zonguldak, TurkeyDepartment of Urology, Zonguldak Karaelmas University, Zonguldak, TurkeyDepartment of Urology, Faculty of Medicine, Yeditepe University, Istanbul, TurkeyDepartment of Biochemistry, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, TurkeyDepartment of Urology, Zonguldak Karaelmas University, Zonguldak, TurkeyWe investigated the effect of intraperitoneal vardenafil (1 mg/kg) administration during an ischemic period in a rat model of testicular torsion/detorsion (T/D). Twenty-one adult Wistar rats were equally randomized into a control group, a T/D group and a vardenafil group. The control group was designed to collect basal values for biochemical and histopathological parameters. The T/D group underwent testicular torsion for 1 hour. The vardenafil group received vardenafil (1mg/kg) intraperitoneally at 30 minutes after torsion. All rats were sacrificed 4 hours after reperfusion to evaluate the tissue levels of malondialdehyde and total antioxidant status. Germ cell apoptosis was evaluated using the apoptosis protease activating factor 1 antibody in all groups. The expressions of endothelial nitric oxide synthase (NOS) and inducible NOS were also assessed in both testes of all rats. The malondialdehyde levels in the T/D group were significantly higher than in the control and vardenafil groups. There were also significant decreases in total antioxidant status in the T/D group compared with the control and vardenafil groups. Vardenafil treatment significantly reduced apoptosis protease activating factor 1, endothelial NOS and inducible NOS levels in the vardenafil group compared with the T/D group. Administration of 1 mg/kg vardenafil during testicular torsion decreased ischemia/reperfusion cellular damage. Our results indicate that the reduction in oxidative stress by vardenafil may play a major role in its cytoprotective effects.http://www.sciencedirect.com/science/article/pii/S1607551X09705303ischemia reperfusiontestes torsionvardenafil |
| spellingShingle | Bulent Erol Husnu Tokgoz Volkan Hanci Sibel Bektas Bulent Akduman Faruk Yencilek Gorkem Mungan Aydin Mungan Vardenafil Reduces Testicular Damage Following Ischemia/Reperfusion Injury in Rats Kaohsiung Journal of Medical Sciences ischemia reperfusion testes torsion vardenafil |
| title | Vardenafil Reduces Testicular Damage Following Ischemia/Reperfusion Injury in Rats |
| title_full | Vardenafil Reduces Testicular Damage Following Ischemia/Reperfusion Injury in Rats |
| title_fullStr | Vardenafil Reduces Testicular Damage Following Ischemia/Reperfusion Injury in Rats |
| title_full_unstemmed | Vardenafil Reduces Testicular Damage Following Ischemia/Reperfusion Injury in Rats |
| title_short | Vardenafil Reduces Testicular Damage Following Ischemia/Reperfusion Injury in Rats |
| title_sort | vardenafil reduces testicular damage following ischemia reperfusion injury in rats |
| topic | ischemia reperfusion testes torsion vardenafil |
| url | http://www.sciencedirect.com/science/article/pii/S1607551X09705303 |
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