Effects of Different Concentrations of Melatonin on the Time-course of Nitrite–induced Oxidation of Hemoglobin: In vitro Study
Melatonin is a potent scavenger of reactive oxygen species or free radicals like superoxide and hydroxyl radicals. The oxidation of hemoglobin to methemoglobin (meth-Hb) by oxidizing compounds has been widely studied. The present work was designed to evaluate the ability of different concentrations...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
College of Pharmacy University of Baghdad
2017-03-01
|
| Series: | Iraqi Journal of Pharmaceutical Sciences |
| Online Access: | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/558 |
| _version_ | 1818883898356006912 |
|---|---|
| author | Saad A.Hussain Shaima M. Mohammed Intesar T. Numan Ihab I. Abdulwahhab |
| author_facet | Saad A.Hussain Shaima M. Mohammed Intesar T. Numan Ihab I. Abdulwahhab |
| author_sort | Saad A.Hussain |
| collection | DOAJ |
| description | Melatonin is a potent scavenger of reactive oxygen species or free radicals like superoxide and hydroxyl radicals. The oxidation of hemoglobin to methemoglobin (meth-Hb) by oxidizing compounds has been widely studied. The present work was designed to evaluate the ability of different concentrations of melatonin to inhibit nitrite–induced oxidation of hemoglobin. Blood samples were obtained from apparently healthy individuals from which erythrocyte hemolysate was prepared. Different concentrations of melatonin (10-9-1.0 mg/ml) were incubated for 10 min with the hemolysate, then to the resultant mixture 1 ml of sodium nitrite (final concentration 0.6 mM) was added, and the formation of meth-Hb was measured by monitoring absorbance of light at 631 nm each min for 30 min. Control samples without melatonin were utilized for comparison. Nitrite caused rapid oxidation of hemoglobin to meth-Hb in control samples; in the presence of melatonin, the oxidation process was delayed in a dose–dependent manner. The effect of melatonin on the time course of nitrite-induced oxidation of Hb showed that melatonin has a protective effect initiated early after addition along with nitrite. Melatonin also affect the time required for the formation of meth-Hb, the time required to convert 50% of the available Hb to meth-Hb was 4 min in the absence of melatonin, and became 17, 22, 26, 30, 114 and 383 min with increasing melatonin concentrations (10-9, 10-6, 0.001, 0.01, 0.1, and 1.0 mg/ml respectively). In conclusion, melatonin in a concentration and time dependent manner can protect Hb from oxidation by nitrite; melatonin delays the onset of autocatalytic stage and the protective effect extended over long period of time.
Key words: melatonin, erythrocytes oxidation |
| first_indexed | 2024-12-19T15:40:58Z |
| format | Article |
| id | doaj.art-fa86400347c94ee299339042f57a715f |
| institution | Directory Open Access Journal |
| issn | 2521-3512 1683-3597 |
| language | English |
| last_indexed | 2024-12-19T15:40:58Z |
| publishDate | 2017-03-01 |
| publisher | College of Pharmacy University of Baghdad |
| record_format | Article |
| series | Iraqi Journal of Pharmaceutical Sciences |
| spelling | doaj.art-fa86400347c94ee299339042f57a715f2022-12-21T20:15:29ZengCollege of Pharmacy University of BaghdadIraqi Journal of Pharmaceutical Sciences2521-35121683-35972017-03-0118110.31351/vol18iss1pp78-81Effects of Different Concentrations of Melatonin on the Time-course of Nitrite–induced Oxidation of Hemoglobin: In vitro StudySaad A.HussainShaima M. MohammedIntesar T. NumanIhab I. AbdulwahhabMelatonin is a potent scavenger of reactive oxygen species or free radicals like superoxide and hydroxyl radicals. The oxidation of hemoglobin to methemoglobin (meth-Hb) by oxidizing compounds has been widely studied. The present work was designed to evaluate the ability of different concentrations of melatonin to inhibit nitrite–induced oxidation of hemoglobin. Blood samples were obtained from apparently healthy individuals from which erythrocyte hemolysate was prepared. Different concentrations of melatonin (10-9-1.0 mg/ml) were incubated for 10 min with the hemolysate, then to the resultant mixture 1 ml of sodium nitrite (final concentration 0.6 mM) was added, and the formation of meth-Hb was measured by monitoring absorbance of light at 631 nm each min for 30 min. Control samples without melatonin were utilized for comparison. Nitrite caused rapid oxidation of hemoglobin to meth-Hb in control samples; in the presence of melatonin, the oxidation process was delayed in a dose–dependent manner. The effect of melatonin on the time course of nitrite-induced oxidation of Hb showed that melatonin has a protective effect initiated early after addition along with nitrite. Melatonin also affect the time required for the formation of meth-Hb, the time required to convert 50% of the available Hb to meth-Hb was 4 min in the absence of melatonin, and became 17, 22, 26, 30, 114 and 383 min with increasing melatonin concentrations (10-9, 10-6, 0.001, 0.01, 0.1, and 1.0 mg/ml respectively). In conclusion, melatonin in a concentration and time dependent manner can protect Hb from oxidation by nitrite; melatonin delays the onset of autocatalytic stage and the protective effect extended over long period of time. Key words: melatonin, erythrocytes oxidationhttps://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/558 |
| spellingShingle | Saad A.Hussain Shaima M. Mohammed Intesar T. Numan Ihab I. Abdulwahhab Effects of Different Concentrations of Melatonin on the Time-course of Nitrite–induced Oxidation of Hemoglobin: In vitro Study Iraqi Journal of Pharmaceutical Sciences |
| title | Effects of Different Concentrations of Melatonin on the Time-course of Nitrite–induced Oxidation of Hemoglobin: In vitro Study |
| title_full | Effects of Different Concentrations of Melatonin on the Time-course of Nitrite–induced Oxidation of Hemoglobin: In vitro Study |
| title_fullStr | Effects of Different Concentrations of Melatonin on the Time-course of Nitrite–induced Oxidation of Hemoglobin: In vitro Study |
| title_full_unstemmed | Effects of Different Concentrations of Melatonin on the Time-course of Nitrite–induced Oxidation of Hemoglobin: In vitro Study |
| title_short | Effects of Different Concentrations of Melatonin on the Time-course of Nitrite–induced Oxidation of Hemoglobin: In vitro Study |
| title_sort | effects of different concentrations of melatonin on the time course of nitritea€ induced oxidation of hemoglobin in vitro study |
| url | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/558 |
| work_keys_str_mv | AT saadahussain effectsofdifferentconcentrationsofmelatoninonthetimecourseofnitriteainducedoxidationofhemoglobininvitrostudy AT shaimammohammed effectsofdifferentconcentrationsofmelatoninonthetimecourseofnitriteainducedoxidationofhemoglobininvitrostudy AT intesartnuman effectsofdifferentconcentrationsofmelatoninonthetimecourseofnitriteainducedoxidationofhemoglobininvitrostudy AT ihabiabdulwahhab effectsofdifferentconcentrationsofmelatoninonthetimecourseofnitriteainducedoxidationofhemoglobininvitrostudy |