Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]
Bacillus velezensis ML122-2 is an antimicrobial-producing strain isolated from the leaf of Assam tea or Miang [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]. The cell-free supernatant (CFS) of strain ML122-2 exhibits a broad-spectrum antimicrobial activity against various Gram-positive and Gra...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.789362/full |
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| author | Patthanasak Rungsirivanich Patthanasak Rungsirivanich Elvina Parlindungan Elvina Parlindungan Paula M. O’Connor Paula M. O’Connor Des Field Des Field Jennifer Mahony Jennifer Mahony Narumol Thongwai Narumol Thongwai Douwe van Sinderen Douwe van Sinderen |
| author_facet | Patthanasak Rungsirivanich Patthanasak Rungsirivanich Elvina Parlindungan Elvina Parlindungan Paula M. O’Connor Paula M. O’Connor Des Field Des Field Jennifer Mahony Jennifer Mahony Narumol Thongwai Narumol Thongwai Douwe van Sinderen Douwe van Sinderen |
| author_sort | Patthanasak Rungsirivanich |
| collection | DOAJ |
| description | Bacillus velezensis ML122-2 is an antimicrobial-producing strain isolated from the leaf of Assam tea or Miang [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]. The cell-free supernatant (CFS) of strain ML122-2 exhibits a broad-spectrum antimicrobial activity against various Gram-positive and Gram-negative bacteria as well as the mold Penicillium expansum. The genome of B. velezensis ML122-2 was sequenced and in silico analysis identified three potential bacteriocin-associated gene clusters, that is, those involved in the production of mersacidin, amylocyclicin, and LCI. Furthermore, six gene clusters exhibiting homology (75–100% DNA sequence identity) to those associated with the secondary metabolites bacilysin, bacillibactin, surfactin, macrolactin H, bacillaene, and plipastatin were identified. Individual antimicrobial activities produced by B. velezensis ML122-2 were purified and characterized by Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry analysis, revealing three antimicrobial peptides with molecular masses corresponding to surfactin, plipastatin, and amylocyclicin. Transcriptional analysis of specific genes associated with mersacidin (mrsA), amylocyclicin (acnA), plipastatin (ppsA), and surfactin (srfAA) production by B. velezensis ML122-2 showed that the first was not transcribed under the conditions tested, while the latter three were consistent with the presence of the associated peptides as determined by mass spectrometry analysis. These findings demonstrate that B. velezensis ML122-2 has the genetic capacity to produce a wide range of antimicrobial activities that may support a specific community structure and highlight the biotechnological properties of Assam tea. |
| first_indexed | 2024-12-14T07:59:55Z |
| format | Article |
| id | doaj.art-fa87c892e8f642a5988fcbe15dbd3bdb |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T07:59:55Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-fa87c892e8f642a5988fcbe15dbd3bdb2022-12-21T23:10:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.789362789362Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]Patthanasak Rungsirivanich0Patthanasak Rungsirivanich1Elvina Parlindungan2Elvina Parlindungan3Paula M. O’Connor4Paula M. O’Connor5Des Field6Des Field7Jennifer Mahony8Jennifer Mahony9Narumol Thongwai10Narumol Thongwai11Douwe van Sinderen12Douwe van Sinderen13Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, ThailandGraduate School, Chiang Mai University, Chiang Mai, ThailandSchool of Microbiology, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, IrelandTeagasc Food Research Centre, Moorepark, Fermoy, IrelandSchool of Microbiology, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, IrelandSchool of Microbiology, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, IrelandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, ThailandResearch Center in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai, ThailandSchool of Microbiology, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, IrelandBacillus velezensis ML122-2 is an antimicrobial-producing strain isolated from the leaf of Assam tea or Miang [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]. The cell-free supernatant (CFS) of strain ML122-2 exhibits a broad-spectrum antimicrobial activity against various Gram-positive and Gram-negative bacteria as well as the mold Penicillium expansum. The genome of B. velezensis ML122-2 was sequenced and in silico analysis identified three potential bacteriocin-associated gene clusters, that is, those involved in the production of mersacidin, amylocyclicin, and LCI. Furthermore, six gene clusters exhibiting homology (75–100% DNA sequence identity) to those associated with the secondary metabolites bacilysin, bacillibactin, surfactin, macrolactin H, bacillaene, and plipastatin were identified. Individual antimicrobial activities produced by B. velezensis ML122-2 were purified and characterized by Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry analysis, revealing three antimicrobial peptides with molecular masses corresponding to surfactin, plipastatin, and amylocyclicin. Transcriptional analysis of specific genes associated with mersacidin (mrsA), amylocyclicin (acnA), plipastatin (ppsA), and surfactin (srfAA) production by B. velezensis ML122-2 showed that the first was not transcribed under the conditions tested, while the latter three were consistent with the presence of the associated peptides as determined by mass spectrometry analysis. These findings demonstrate that B. velezensis ML122-2 has the genetic capacity to produce a wide range of antimicrobial activities that may support a specific community structure and highlight the biotechnological properties of Assam tea.https://www.frontiersin.org/articles/10.3389/fmicb.2021.789362/fullamylocyclicinbacteriocinbiocontrolgene clusterMiangplipastatin |
| spellingShingle | Patthanasak Rungsirivanich Patthanasak Rungsirivanich Elvina Parlindungan Elvina Parlindungan Paula M. O’Connor Paula M. O’Connor Des Field Des Field Jennifer Mahony Jennifer Mahony Narumol Thongwai Narumol Thongwai Douwe van Sinderen Douwe van Sinderen Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.] Frontiers in Microbiology amylocyclicin bacteriocin biocontrol gene cluster Miang plipastatin |
| title | Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.] |
| title_full | Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.] |
| title_fullStr | Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.] |
| title_full_unstemmed | Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.] |
| title_short | Simultaneous Production of Multiple Antimicrobial Compounds by Bacillus velezensis ML122-2 Isolated From Assam Tea Leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.] |
| title_sort | simultaneous production of multiple antimicrobial compounds by bacillus velezensis ml122 2 isolated from assam tea leaf camellia sinensis var assamica j w mast kitam |
| topic | amylocyclicin bacteriocin biocontrol gene cluster Miang plipastatin |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.789362/full |
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