B.infantis enhances immunotherapy for Guillain-Barre syndrome through regulating PD-1
Abstract Background Guillain-Barré syndrome (GBS) is a rare, autoimmune disease. B.infantis is reported to be effective in alleviating GBS by regulating abnormal function of T helper (Th) cells. Objectives In this study, T cells were isolated from healthy and GBS patients. The therapeutic effect of...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Neurology |
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| Online Access: | https://doi.org/10.1186/s12883-022-03046-w |
| _version_ | 1811175851341905920 |
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| author | Peng Shi Di Nian Hongdang Qu Ming Ye Chun Wang Li Li Qian Li |
| author_facet | Peng Shi Di Nian Hongdang Qu Ming Ye Chun Wang Li Li Qian Li |
| author_sort | Peng Shi |
| collection | DOAJ |
| description | Abstract Background Guillain-Barré syndrome (GBS) is a rare, autoimmune disease. B.infantis is reported to be effective in alleviating GBS by regulating abnormal function of T helper (Th) cells. Objectives In this study, T cells were isolated from healthy and GBS patients. The therapeutic effect of Bifidobacterium infantis (B.infantis) and whether it is achieved by PD-1 was examined at cellular and animal models. Methods We used CCK-8, flow cytometry and real-time PCR to determine the differentiation of T cell subsets at cellular level. Then, an experimental autoimmune neuritis (EAN) animal model using six-week SD rats (n = 30, male) weighing 180–200 g was established to support the role of B. infantis in GBS through PD-1. Results B. infantis inhibited the proliferation and promoted apoptosis of T cells from GBS. At the same time, the expression levels of PD-1 increased, which was correlated with decreased T-bet (Th1) and ROR-γt (Th17) and increased Foxp3 (Treg) expression. Moreover, B. infantis alleviated the symptoms of GBS. Th1 and Th17 cells decreased while Treg cells increased after B. infantis treatment, which could be partly abrogated by PD-1 inhibitor. Conclusions We concluded from this study that B.infantis alleviated GBS partly through PD-1. |
| first_indexed | 2024-04-10T19:42:36Z |
| format | Article |
| id | doaj.art-fa8aa95bfc894a6389468d810f87d386 |
| institution | Directory Open Access Journal |
| issn | 1471-2377 |
| language | English |
| last_indexed | 2024-04-10T19:42:36Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neurology |
| spelling | doaj.art-fa8aa95bfc894a6389468d810f87d3862023-01-29T12:15:37ZengBMCBMC Neurology1471-23772023-01-012311810.1186/s12883-022-03046-wB.infantis enhances immunotherapy for Guillain-Barre syndrome through regulating PD-1Peng Shi0Di Nian1Hongdang Qu2Ming Ye3Chun Wang4Li Li5Qian Li6Department of Neurology, first Affiliated Hospital of Bengbu Medical CollegeDepartment of Medical Examination, Bengbu Medical CollegeDepartment of Neurology, first Affiliated Hospital of Bengbu Medical CollegeDepartment of Neurology, first Affiliated Hospital of Bengbu Medical CollegeDepartment of Neurology, first Affiliated Hospital of Bengbu Medical CollegeDepartment of Neurology, first Affiliated Hospital of Bengbu Medical CollegeDepartment of Neurology, first Affiliated Hospital of Bengbu Medical CollegeAbstract Background Guillain-Barré syndrome (GBS) is a rare, autoimmune disease. B.infantis is reported to be effective in alleviating GBS by regulating abnormal function of T helper (Th) cells. Objectives In this study, T cells were isolated from healthy and GBS patients. The therapeutic effect of Bifidobacterium infantis (B.infantis) and whether it is achieved by PD-1 was examined at cellular and animal models. Methods We used CCK-8, flow cytometry and real-time PCR to determine the differentiation of T cell subsets at cellular level. Then, an experimental autoimmune neuritis (EAN) animal model using six-week SD rats (n = 30, male) weighing 180–200 g was established to support the role of B. infantis in GBS through PD-1. Results B. infantis inhibited the proliferation and promoted apoptosis of T cells from GBS. At the same time, the expression levels of PD-1 increased, which was correlated with decreased T-bet (Th1) and ROR-γt (Th17) and increased Foxp3 (Treg) expression. Moreover, B. infantis alleviated the symptoms of GBS. Th1 and Th17 cells decreased while Treg cells increased after B. infantis treatment, which could be partly abrogated by PD-1 inhibitor. Conclusions We concluded from this study that B.infantis alleviated GBS partly through PD-1.https://doi.org/10.1186/s12883-022-03046-wGuillain-Barré syndromeB. infantis,experimental autoimmune neuritis animal modelT helper cellsPD-1 |
| spellingShingle | Peng Shi Di Nian Hongdang Qu Ming Ye Chun Wang Li Li Qian Li B.infantis enhances immunotherapy for Guillain-Barre syndrome through regulating PD-1 BMC Neurology Guillain-Barré syndrome B. infantis,experimental autoimmune neuritis animal model T helper cells PD-1 |
| title | B.infantis enhances immunotherapy for Guillain-Barre syndrome through regulating PD-1 |
| title_full | B.infantis enhances immunotherapy for Guillain-Barre syndrome through regulating PD-1 |
| title_fullStr | B.infantis enhances immunotherapy for Guillain-Barre syndrome through regulating PD-1 |
| title_full_unstemmed | B.infantis enhances immunotherapy for Guillain-Barre syndrome through regulating PD-1 |
| title_short | B.infantis enhances immunotherapy for Guillain-Barre syndrome through regulating PD-1 |
| title_sort | b infantis enhances immunotherapy for guillain barre syndrome through regulating pd 1 |
| topic | Guillain-Barré syndrome B. infantis,experimental autoimmune neuritis animal model T helper cells PD-1 |
| url | https://doi.org/10.1186/s12883-022-03046-w |
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