The Effects of Andrographolide on the Enhancement of Chondrogenesis and Osteogenesis in Human Suprapatellar Fat Pad Derived Mesenchymal Stem Cells
Andrographolide is a labdane diterpenoid herb, which is isolated from the leaves of <i>Andrographis paniculata,</i> and widely used for its potential medical properties. However, there are no reports on the effects of andrographolide on the human suprapatellar fat pad of osteoarthritis p...
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/7/1831 |
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| author | Thitianan Kulsirirat Sittisak Honsawek Mariko Takeda-Morishita Nuttanan Sinchaipanid Wanvisa Udomsinprasert Jiraporn Leanpolchareanchai Korbtham Sathirakul |
| author_facet | Thitianan Kulsirirat Sittisak Honsawek Mariko Takeda-Morishita Nuttanan Sinchaipanid Wanvisa Udomsinprasert Jiraporn Leanpolchareanchai Korbtham Sathirakul |
| author_sort | Thitianan Kulsirirat |
| collection | DOAJ |
| description | Andrographolide is a labdane diterpenoid herb, which is isolated from the leaves of <i>Andrographis paniculata,</i> and widely used for its potential medical properties. However, there are no reports on the effects of andrographolide on the human suprapatellar fat pad of osteoarthritis patients. In the present study, our goal was to evaluate the innovative effects of andrographolide on viability and Tri-lineage differentiation of human mesenchymal stem cells from suprapatellar fat pad tissues. The results revealed that andrographolide had no cytotoxic effects when the concentration was less than 12.5 µM. Interestingly, andrographolide had significantly enhanced, dose dependent, osteogenesis and chondrogenesis as evidenced by a significantly intensified stain for Alizarin Red S, Toluidine Blue and Alcian Blue. Moreover, andrographolide can upregulate the expression of genes related to osteogenic and chondrogenic differentiation, including <i>Runx2</i>, <i>OPN</i>, <i>Sox9</i>, and <i>Aggrecan</i> in mesenchymal stem cells from human suprapatellar fat pad tissues. In contrast, andrographolide suppressed adipogenic differentiation as evidenced by significantly diminished Oil Red O staining and expression levels for adipogenic-specific genes for <i>PPAR-γ2</i> and <i>LPL</i>. These findings confirm that andrographolide can specifically enhance osteogenesis and chondrogenesis of mesenchymal stem cells from human suprapatellar fat pad tissues. It has potential as a therapeutic agent derived from natural sources for regenerative medicine. |
| first_indexed | 2024-03-10T12:56:39Z |
| format | Article |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T12:56:39Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-fa8b213de915465786155d1a13681fe12023-11-21T11:51:23ZengMDPI AGMolecules1420-30492021-03-01267183110.3390/molecules26071831The Effects of Andrographolide on the Enhancement of Chondrogenesis and Osteogenesis in Human Suprapatellar Fat Pad Derived Mesenchymal Stem CellsThitianan Kulsirirat0Sittisak Honsawek1Mariko Takeda-Morishita2Nuttanan Sinchaipanid3Wanvisa Udomsinprasert4Jiraporn Leanpolchareanchai5Korbtham Sathirakul6Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandOsteoarthritis and Musculoskeleton Research Unit, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandLaboratory of Drug Delivery Systems, Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Kobe, Hyogo 650-8586, JapanDepartment of Manufacturing Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandDepartment of Biochemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandDepartment of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandDepartment of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandAndrographolide is a labdane diterpenoid herb, which is isolated from the leaves of <i>Andrographis paniculata,</i> and widely used for its potential medical properties. However, there are no reports on the effects of andrographolide on the human suprapatellar fat pad of osteoarthritis patients. In the present study, our goal was to evaluate the innovative effects of andrographolide on viability and Tri-lineage differentiation of human mesenchymal stem cells from suprapatellar fat pad tissues. The results revealed that andrographolide had no cytotoxic effects when the concentration was less than 12.5 µM. Interestingly, andrographolide had significantly enhanced, dose dependent, osteogenesis and chondrogenesis as evidenced by a significantly intensified stain for Alizarin Red S, Toluidine Blue and Alcian Blue. Moreover, andrographolide can upregulate the expression of genes related to osteogenic and chondrogenic differentiation, including <i>Runx2</i>, <i>OPN</i>, <i>Sox9</i>, and <i>Aggrecan</i> in mesenchymal stem cells from human suprapatellar fat pad tissues. In contrast, andrographolide suppressed adipogenic differentiation as evidenced by significantly diminished Oil Red O staining and expression levels for adipogenic-specific genes for <i>PPAR-γ2</i> and <i>LPL</i>. These findings confirm that andrographolide can specifically enhance osteogenesis and chondrogenesis of mesenchymal stem cells from human suprapatellar fat pad tissues. It has potential as a therapeutic agent derived from natural sources for regenerative medicine.https://www.mdpi.com/1420-3049/26/7/1831mesenchymal stem cellssuprapatellar fat padandrographolideosteoarthritisregenerative medicine |
| spellingShingle | Thitianan Kulsirirat Sittisak Honsawek Mariko Takeda-Morishita Nuttanan Sinchaipanid Wanvisa Udomsinprasert Jiraporn Leanpolchareanchai Korbtham Sathirakul The Effects of Andrographolide on the Enhancement of Chondrogenesis and Osteogenesis in Human Suprapatellar Fat Pad Derived Mesenchymal Stem Cells Molecules mesenchymal stem cells suprapatellar fat pad andrographolide osteoarthritis regenerative medicine |
| title | The Effects of Andrographolide on the Enhancement of Chondrogenesis and Osteogenesis in Human Suprapatellar Fat Pad Derived Mesenchymal Stem Cells |
| title_full | The Effects of Andrographolide on the Enhancement of Chondrogenesis and Osteogenesis in Human Suprapatellar Fat Pad Derived Mesenchymal Stem Cells |
| title_fullStr | The Effects of Andrographolide on the Enhancement of Chondrogenesis and Osteogenesis in Human Suprapatellar Fat Pad Derived Mesenchymal Stem Cells |
| title_full_unstemmed | The Effects of Andrographolide on the Enhancement of Chondrogenesis and Osteogenesis in Human Suprapatellar Fat Pad Derived Mesenchymal Stem Cells |
| title_short | The Effects of Andrographolide on the Enhancement of Chondrogenesis and Osteogenesis in Human Suprapatellar Fat Pad Derived Mesenchymal Stem Cells |
| title_sort | effects of andrographolide on the enhancement of chondrogenesis and osteogenesis in human suprapatellar fat pad derived mesenchymal stem cells |
| topic | mesenchymal stem cells suprapatellar fat pad andrographolide osteoarthritis regenerative medicine |
| url | https://www.mdpi.com/1420-3049/26/7/1831 |
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