Investigation and Scaling of Hydrogen Production by Klebsiella sp. ABZ11 for Optimal Yield and the Kinetics of Batch Fermentation Process
The slow metabolism of bacteria at low temperatures affects the catalytic efficiency of enzymes and productivity. This article investigates the use of a psychrotolerant bacteria (Klebsiella sp. ABZ11) for biohydrogen production, yield and scaling at optimal temperature, pH and glucose in a batch fe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2022-12-01
|
| Series: | Journal of Applied Sciences and Environmental Management |
| Subjects: | |
| Online Access: | https://www.ajol.info/index.php/jasem/article/view/239344 |
| _version_ | 1827297361810948096 |
|---|---|
| author | A. Mohammed A. Mohd Firdaus I. Zaharah I. L. Mohammed J. N. Mohammed T. V. Balogun |
| author_facet | A. Mohammed A. Mohd Firdaus I. Zaharah I. L. Mohammed J. N. Mohammed T. V. Balogun |
| author_sort | A. Mohammed |
| collection | DOAJ |
| description |
The slow metabolism of bacteria at low temperatures affects the catalytic efficiency of enzymes and productivity. This article investigates the use of a psychrotolerant bacteria (Klebsiella sp. ABZ11) for biohydrogen production, yield and scaling at optimal temperature, pH and glucose in a batch fermentation process within a 2-liter bioreactor using Response Surface Methodology (RSM) to report the process performance. The results show 33.5°C, pH 6.75 and 9.15 g glucose as the optimal conditions. Scale-up yielded 137.56 mol/L biohydrogen, 22.13% more than production under optimized conditions. Biomass grew at 0.081/h and doubled in 17 h with 0.71 g cells to reach maximum production. Compared to 0.062/h, 22 h with 0.87 g cells in optimal condition to achieve maximum biohydrogen production. This result shows the potential of biohydrogen production using Antarctic psychrotolerant bacteria at mesophilic temperature.
|
| first_indexed | 2024-04-24T14:54:05Z |
| format | Article |
| id | doaj.art-fa91b33a4751452c9c948eb65a9d5531 |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T14:54:05Z |
| publishDate | 2022-12-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-fa91b33a4751452c9c948eb65a9d55312024-04-02T19:46:17ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992022-12-01261210.4314/jasem.v26i12.28Investigation and Scaling of Hydrogen Production by Klebsiella sp. ABZ11 for Optimal Yield and the Kinetics of Batch Fermentation ProcessA. MohammedA. Mohd FirdausI. ZaharahI. L. MohammedJ. N. MohammedT. V. Balogun The slow metabolism of bacteria at low temperatures affects the catalytic efficiency of enzymes and productivity. This article investigates the use of a psychrotolerant bacteria (Klebsiella sp. ABZ11) for biohydrogen production, yield and scaling at optimal temperature, pH and glucose in a batch fermentation process within a 2-liter bioreactor using Response Surface Methodology (RSM) to report the process performance. The results show 33.5°C, pH 6.75 and 9.15 g glucose as the optimal conditions. Scale-up yielded 137.56 mol/L biohydrogen, 22.13% more than production under optimized conditions. Biomass grew at 0.081/h and doubled in 17 h with 0.71 g cells to reach maximum production. Compared to 0.062/h, 22 h with 0.87 g cells in optimal condition to achieve maximum biohydrogen production. This result shows the potential of biohydrogen production using Antarctic psychrotolerant bacteria at mesophilic temperature. https://www.ajol.info/index.php/jasem/article/view/239344Biohydrogen production;Optimization;Scale-up;Kinetics |
| spellingShingle | A. Mohammed A. Mohd Firdaus I. Zaharah I. L. Mohammed J. N. Mohammed T. V. Balogun Investigation and Scaling of Hydrogen Production by Klebsiella sp. ABZ11 for Optimal Yield and the Kinetics of Batch Fermentation Process Journal of Applied Sciences and Environmental Management Biohydrogen production; Optimization; Scale-up; Kinetics |
| title | Investigation and Scaling of Hydrogen Production by Klebsiella sp. ABZ11 for Optimal Yield and the Kinetics of Batch Fermentation Process |
| title_full | Investigation and Scaling of Hydrogen Production by Klebsiella sp. ABZ11 for Optimal Yield and the Kinetics of Batch Fermentation Process |
| title_fullStr | Investigation and Scaling of Hydrogen Production by Klebsiella sp. ABZ11 for Optimal Yield and the Kinetics of Batch Fermentation Process |
| title_full_unstemmed | Investigation and Scaling of Hydrogen Production by Klebsiella sp. ABZ11 for Optimal Yield and the Kinetics of Batch Fermentation Process |
| title_short | Investigation and Scaling of Hydrogen Production by Klebsiella sp. ABZ11 for Optimal Yield and the Kinetics of Batch Fermentation Process |
| title_sort | investigation and scaling of hydrogen production by klebsiella sp abz11 for optimal yield and the kinetics of batch fermentation process |
| topic | Biohydrogen production; Optimization; Scale-up; Kinetics |
| url | https://www.ajol.info/index.php/jasem/article/view/239344 |
| work_keys_str_mv | AT amohammed investigationandscalingofhydrogenproductionbyklebsiellaspabz11foroptimalyieldandthekineticsofbatchfermentationprocess AT amohdfirdaus investigationandscalingofhydrogenproductionbyklebsiellaspabz11foroptimalyieldandthekineticsofbatchfermentationprocess AT izaharah investigationandscalingofhydrogenproductionbyklebsiellaspabz11foroptimalyieldandthekineticsofbatchfermentationprocess AT ilmohammed investigationandscalingofhydrogenproductionbyklebsiellaspabz11foroptimalyieldandthekineticsofbatchfermentationprocess AT jnmohammed investigationandscalingofhydrogenproductionbyklebsiellaspabz11foroptimalyieldandthekineticsofbatchfermentationprocess AT tvbalogun investigationandscalingofhydrogenproductionbyklebsiellaspabz11foroptimalyieldandthekineticsofbatchfermentationprocess |