Polarized SCAR and the Arp2/3 complex regulate apical cortical remodeling in asymmetrically dividing neuroblasts
Summary: Although the formin-nucleated actomyosin cortex has been shown to drive the changes in cell shape that accompany animal cell division in both symmetric and asymmetric cell divisions, the mitotic role of cortical Arp2/3-nucleated actin networks remain unclear. Here using asymmetrically divid...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223012063 |
| _version_ | 1797774587283374080 |
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| author | Giulia Cazzagon Chantal Roubinet Buzz Baum |
| author_facet | Giulia Cazzagon Chantal Roubinet Buzz Baum |
| author_sort | Giulia Cazzagon |
| collection | DOAJ |
| description | Summary: Although the formin-nucleated actomyosin cortex has been shown to drive the changes in cell shape that accompany animal cell division in both symmetric and asymmetric cell divisions, the mitotic role of cortical Arp2/3-nucleated actin networks remain unclear. Here using asymmetrically dividing Drosophila neural stem cells as a model system, we identify a pool of membrane protrusions that form at the apical cortex of neuroblasts as they enter mitosis. Strikingly, these apically localized protrusions are enriched in SCAR, and depend on SCAR and Arp2/3 complexes for their formation. Because compromising SCAR or the Arp2/3 complex delays the apical clearance of Myosin II at the onset of anaphase and induces cortical instability at cytokinesis, these data point to a role for an apical branched actin filament network in fine-tuning the actomyosin cortex to enable the precise control of cell shape changes during an asymmetric cell division. |
| first_indexed | 2024-03-12T22:23:16Z |
| format | Article |
| id | doaj.art-fa91e575293c4fb39f48630e888f82ae |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:23:16Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-fa91e575293c4fb39f48630e888f82ae2023-07-23T04:55:39ZengElsevieriScience2589-00422023-07-01267107129Polarized SCAR and the Arp2/3 complex regulate apical cortical remodeling in asymmetrically dividing neuroblastsGiulia Cazzagon0Chantal Roubinet1Buzz Baum2Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, UKMedical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Corresponding authorMedical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Corresponding authorSummary: Although the formin-nucleated actomyosin cortex has been shown to drive the changes in cell shape that accompany animal cell division in both symmetric and asymmetric cell divisions, the mitotic role of cortical Arp2/3-nucleated actin networks remain unclear. Here using asymmetrically dividing Drosophila neural stem cells as a model system, we identify a pool of membrane protrusions that form at the apical cortex of neuroblasts as they enter mitosis. Strikingly, these apically localized protrusions are enriched in SCAR, and depend on SCAR and Arp2/3 complexes for their formation. Because compromising SCAR or the Arp2/3 complex delays the apical clearance of Myosin II at the onset of anaphase and induces cortical instability at cytokinesis, these data point to a role for an apical branched actin filament network in fine-tuning the actomyosin cortex to enable the precise control of cell shape changes during an asymmetric cell division.http://www.sciencedirect.com/science/article/pii/S2589004223012063Biological sciencesMolecular biologyNeuroscienceCell biology |
| spellingShingle | Giulia Cazzagon Chantal Roubinet Buzz Baum Polarized SCAR and the Arp2/3 complex regulate apical cortical remodeling in asymmetrically dividing neuroblasts iScience Biological sciences Molecular biology Neuroscience Cell biology |
| title | Polarized SCAR and the Arp2/3 complex regulate apical cortical remodeling in asymmetrically dividing neuroblasts |
| title_full | Polarized SCAR and the Arp2/3 complex regulate apical cortical remodeling in asymmetrically dividing neuroblasts |
| title_fullStr | Polarized SCAR and the Arp2/3 complex regulate apical cortical remodeling in asymmetrically dividing neuroblasts |
| title_full_unstemmed | Polarized SCAR and the Arp2/3 complex regulate apical cortical remodeling in asymmetrically dividing neuroblasts |
| title_short | Polarized SCAR and the Arp2/3 complex regulate apical cortical remodeling in asymmetrically dividing neuroblasts |
| title_sort | polarized scar and the arp2 3 complex regulate apical cortical remodeling in asymmetrically dividing neuroblasts |
| topic | Biological sciences Molecular biology Neuroscience Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223012063 |
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