Influence of the cultivation conditions on ligninolytic enzyme production in Pleurotus pulmonarius
The highest level of laccase activity (391 Ul-1), as well as significant Mn-oxidizing peroxidases production, were found in solid-state culture with grapevine sawdust as the carbon source. After purification of extracellular crude enzyme mixture of Pleurotus pulmonarius, grown in the medium with the...
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| Format: | Article |
| Language: | English |
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Matica srpska
2007-01-01
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| Series: | Zbornik Matice Srpske za Prirodne Nauke |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-4906/2007/0352-49060713303S.pdf |
| _version_ | 1828942502713884672 |
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| author | Stajić Mirjana Vukojević Jelena B. Duletić-Laušević Sonja |
| author_facet | Stajić Mirjana Vukojević Jelena B. Duletić-Laušević Sonja |
| author_sort | Stajić Mirjana |
| collection | DOAJ |
| description | The highest level of laccase activity (391 Ul-1), as well as significant Mn-oxidizing peroxidases production, were found in solid-state culture with grapevine sawdust as the carbon source. After purification of extracellular crude enzyme mixture of Pleurotus pulmonarius, grown in the medium with the best carbon source (grapevine sawdust), three peaks of laccase activity were noted. The results obtained by purification also showed that the levels of phenol red oxidation, in absence of external Mn2+, were higher than phenol red oxidation levels in presence of external Mn2+. The highest laccase activity was in the medium with grapevine sawdust, as carbon source, and NH4Cl at a nitrogen concentration of 30 mM (441 Ul-1). The best nitrogen source for Mn-oxidizing peroxidase production was NH4NO3 at nitrogen concentration of 30 mM. The highest laccase activity was found in the presence of 5 mM Cu2+, and 5 mM Mn2+, respectively. The absence of Cu2+ and Mn2+, as well as their presence at the concentration of 1 mM, led to the peaks of Mn-oxidizing peroxidases activities. Zn2+ and Fe2+ caused a decrease, and Se, in all investigated forms, an increase of laccase and peroxidases activities. |
| first_indexed | 2024-12-14T03:56:29Z |
| format | Article |
| id | doaj.art-fa936f9d4cfb4d218dfe818fafdffe46 |
| institution | Directory Open Access Journal |
| issn | 0352-4906 |
| language | English |
| last_indexed | 2024-12-14T03:56:29Z |
| publishDate | 2007-01-01 |
| publisher | Matica srpska |
| record_format | Article |
| series | Zbornik Matice Srpske za Prirodne Nauke |
| spelling | doaj.art-fa936f9d4cfb4d218dfe818fafdffe462022-12-21T23:18:04ZengMatica srpskaZbornik Matice Srpske za Prirodne Nauke0352-49062007-01-01200711330331210.2298/ZMSPN0713303SInfluence of the cultivation conditions on ligninolytic enzyme production in Pleurotus pulmonariusStajić MirjanaVukojević Jelena B.Duletić-Laušević SonjaThe highest level of laccase activity (391 Ul-1), as well as significant Mn-oxidizing peroxidases production, were found in solid-state culture with grapevine sawdust as the carbon source. After purification of extracellular crude enzyme mixture of Pleurotus pulmonarius, grown in the medium with the best carbon source (grapevine sawdust), three peaks of laccase activity were noted. The results obtained by purification also showed that the levels of phenol red oxidation, in absence of external Mn2+, were higher than phenol red oxidation levels in presence of external Mn2+. The highest laccase activity was in the medium with grapevine sawdust, as carbon source, and NH4Cl at a nitrogen concentration of 30 mM (441 Ul-1). The best nitrogen source for Mn-oxidizing peroxidase production was NH4NO3 at nitrogen concentration of 30 mM. The highest laccase activity was found in the presence of 5 mM Cu2+, and 5 mM Mn2+, respectively. The absence of Cu2+ and Mn2+, as well as their presence at the concentration of 1 mM, led to the peaks of Mn-oxidizing peroxidases activities. Zn2+ and Fe2+ caused a decrease, and Se, in all investigated forms, an increase of laccase and peroxidases activities.http://www.doiserbia.nb.rs/img/doi/0352-4906/2007/0352-49060713303S.pdfPleurotus pulmonariuslaccaseMn-oxidizing peroxidasesraw plant materialscarbon and nitrogen sourcesmicroelements |
| spellingShingle | Stajić Mirjana Vukojević Jelena B. Duletić-Laušević Sonja Influence of the cultivation conditions on ligninolytic enzyme production in Pleurotus pulmonarius Zbornik Matice Srpske za Prirodne Nauke Pleurotus pulmonarius laccase Mn-oxidizing peroxidases raw plant materials carbon and nitrogen sources microelements |
| title | Influence of the cultivation conditions on ligninolytic enzyme production in Pleurotus pulmonarius |
| title_full | Influence of the cultivation conditions on ligninolytic enzyme production in Pleurotus pulmonarius |
| title_fullStr | Influence of the cultivation conditions on ligninolytic enzyme production in Pleurotus pulmonarius |
| title_full_unstemmed | Influence of the cultivation conditions on ligninolytic enzyme production in Pleurotus pulmonarius |
| title_short | Influence of the cultivation conditions on ligninolytic enzyme production in Pleurotus pulmonarius |
| title_sort | influence of the cultivation conditions on ligninolytic enzyme production in pleurotus pulmonarius |
| topic | Pleurotus pulmonarius laccase Mn-oxidizing peroxidases raw plant materials carbon and nitrogen sources microelements |
| url | http://www.doiserbia.nb.rs/img/doi/0352-4906/2007/0352-49060713303S.pdf |
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