Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)
Abstract Background Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols...
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| Format: | Article |
| Language: | English |
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BMC
2017-06-01
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| Series: | Biological Research |
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| Online Access: | http://link.springer.com/article/10.1186/s40659-017-0125-8 |
| _version_ | 1818412696449581056 |
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| author | Karla A. Quiroz Miguel Berríos Basilio Carrasco Jorge B. Retamales Peter D. S. Caligari Rolando García-Gonzáles |
| author_facet | Karla A. Quiroz Miguel Berríos Basilio Carrasco Jorge B. Retamales Peter D. S. Caligari Rolando García-Gonzáles |
| author_sort | Karla A. Quiroz |
| collection | DOAJ |
| description | Abstract Background Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. Results In this context, we studied the cultivation of meristems and establishment of a micropropagation protocol for F. chiloensis. The addition of polyvinylpyrrolidone (PVP) improved the meristem regeneration efficiency of F. chiloensis accessions. Similarly, the use of 6-benzylaminopurine (BAP) in the culture media increased the average rate of multiplication to 3–6 shoots per plant. In addition, the use of 6-benzylaminopurine (BAP), had low levels (near zero) of explant losses due to oxidation. However, plant height as well as number of leaves and roots were higher in media without growth regulators, with average values of 0.5 cm, 9 leaves and 4 roots per plant. Conclusions For the first time in Chilean strawberry, meristem culture demonstrated to be an efficient tool for eliminating virus from infected plants, giving the possibility to produce disease free propagation material. Also, the addition of PVP into the basal MS medium improved the efficiency of plant recovery from isolated meristems. Farmers can now access to high quality plant material produced by biotech tools which will improve their technological practices. |
| first_indexed | 2024-12-14T10:51:25Z |
| format | Article |
| id | doaj.art-fa9451fd0586468ab4710a68174e8686 |
| institution | Directory Open Access Journal |
| issn | 0717-6287 |
| language | English |
| last_indexed | 2024-12-14T10:51:25Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-fa9451fd0586468ab4710a68174e86862022-12-21T23:05:12ZengBMCBiological Research0717-62872017-06-0150111110.1186/s40659-017-0125-8Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)Karla A. Quiroz0Miguel Berríos1Basilio Carrasco2Jorge B. Retamales3Peter D. S. Caligari4Rolando García-Gonzáles5Instituto de Biología Vegetal y Biotecnología, Universidad de TalcaCentro de Biotecnología de los Recursos Naturales (CENBio), Facultad de Ciencias Agrarias y Forestales, Universidad Católica del MauleFacultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de ChileCentro de Mejoramiento Genético y Fenómica Vegetal, Universidad de TalcaInstituto de Biología Vegetal y Biotecnología, Universidad de TalcaSociedad de Investigación y Servicios, BioTECNOS Ltda.Abstract Background Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. Results In this context, we studied the cultivation of meristems and establishment of a micropropagation protocol for F. chiloensis. The addition of polyvinylpyrrolidone (PVP) improved the meristem regeneration efficiency of F. chiloensis accessions. Similarly, the use of 6-benzylaminopurine (BAP) in the culture media increased the average rate of multiplication to 3–6 shoots per plant. In addition, the use of 6-benzylaminopurine (BAP), had low levels (near zero) of explant losses due to oxidation. However, plant height as well as number of leaves and roots were higher in media without growth regulators, with average values of 0.5 cm, 9 leaves and 4 roots per plant. Conclusions For the first time in Chilean strawberry, meristem culture demonstrated to be an efficient tool for eliminating virus from infected plants, giving the possibility to produce disease free propagation material. Also, the addition of PVP into the basal MS medium improved the efficiency of plant recovery from isolated meristems. Farmers can now access to high quality plant material produced by biotech tools which will improve their technological practices.http://link.springer.com/article/10.1186/s40659-017-0125-8Fragaria chiloensisMeristem culturePlant growth regulatorsVirus eliminationPlant morphogenesis |
| spellingShingle | Karla A. Quiroz Miguel Berríos Basilio Carrasco Jorge B. Retamales Peter D. S. Caligari Rolando García-Gonzáles Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.) Biological Research Fragaria chiloensis Meristem culture Plant growth regulators Virus elimination Plant morphogenesis |
| title | Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.) |
| title_full | Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.) |
| title_fullStr | Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.) |
| title_full_unstemmed | Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.) |
| title_short | Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.) |
| title_sort | meristem culture and subsequent micropropagation of chilean strawberry fragaria chiloensis l duch |
| topic | Fragaria chiloensis Meristem culture Plant growth regulators Virus elimination Plant morphogenesis |
| url | http://link.springer.com/article/10.1186/s40659-017-0125-8 |
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