Molecular aspects of Chikungunya virus infections in cancer patients
BACKGROUND Chikungunya virus (CHIKV) is an arbovirus that can cause chronic and debilitating manifestations. The first autochthonous case in Rio de Janeiro state was diagnosed in 2015, and an outbreak was declared in 2016. OBJECTIVE The aim of this work was to evaluate CHIKV viral load in serum,...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2022-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762022000101103&tlng=en |
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| author | Débora Familiar-Macedo Bianca Ervatti Gama Vanessa Erichsen Emmel Gabriela Vera-Lozada Eliana Abdelhay Ianick Souto Martins Rocio Hassan |
| author_facet | Débora Familiar-Macedo Bianca Ervatti Gama Vanessa Erichsen Emmel Gabriela Vera-Lozada Eliana Abdelhay Ianick Souto Martins Rocio Hassan |
| author_sort | Débora Familiar-Macedo |
| collection | DOAJ |
| description | BACKGROUND Chikungunya virus (CHIKV) is an arbovirus that can cause chronic and debilitating manifestations. The first autochthonous case in Rio de Janeiro state was diagnosed in 2015, and an outbreak was declared in 2016. OBJECTIVE The aim of this work was to evaluate CHIKV viral load in serum, plasma and urine in cancer patients to determine the best sample for diagnosis, as well as perform molecular characterisation and phylogenetic analysis of circulating strains. METHODS Paired serum, plasma and urine collected from 31 cancer patients were tested by real-time quantitative polymerase chain reaction (qPCR) and a segment of the CHIKV E1 gene was sequenced. FINDINGS We detected 11 CHIKV+ oncological patients. Paired samples analyses of nine patients showed a different pattern of detection. Also, a higher viral load in plasma (6.84 log10) and serum (6.07 log10) vs urine (3.76 log10) was found. Phylogenetic analysis and molecular characterisation revealed East/Central/Southern Africa (ECSA) genotype circulation and three amino acids substitutions (E1-K211T, E1-M269V, E1-T288I) in positive patients. MAIN CONCLUSION The results indicate the bioequivalence of serum and plasma for CHIKV diagnosis, with urine being an important complement. ECSA genotype was circulating among patients in the period of the 2016 outbreak with K211T, M269V and T288I substitution. |
| first_indexed | 2024-03-12T09:24:50Z |
| format | Article |
| id | doaj.art-fa9456bbac6440a99c7bf88f095448f6 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:24:50Z |
| publishDate | 2022-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-fa9456bbac6440a99c7bf88f095448f62023-09-02T14:21:06ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602022-04-0111710.1590/0074-02760210383Molecular aspects of Chikungunya virus infections in cancer patientsDébora Familiar-Macedohttps://orcid.org/0000-0002-9229-5912Bianca Ervatti GamaVanessa Erichsen EmmelGabriela Vera-LozadaEliana AbdelhayIanick Souto MartinsRocio Hassan BACKGROUND Chikungunya virus (CHIKV) is an arbovirus that can cause chronic and debilitating manifestations. The first autochthonous case in Rio de Janeiro state was diagnosed in 2015, and an outbreak was declared in 2016. OBJECTIVE The aim of this work was to evaluate CHIKV viral load in serum, plasma and urine in cancer patients to determine the best sample for diagnosis, as well as perform molecular characterisation and phylogenetic analysis of circulating strains. METHODS Paired serum, plasma and urine collected from 31 cancer patients were tested by real-time quantitative polymerase chain reaction (qPCR) and a segment of the CHIKV E1 gene was sequenced. FINDINGS We detected 11 CHIKV+ oncological patients. Paired samples analyses of nine patients showed a different pattern of detection. Also, a higher viral load in plasma (6.84 log10) and serum (6.07 log10) vs urine (3.76 log10) was found. Phylogenetic analysis and molecular characterisation revealed East/Central/Southern Africa (ECSA) genotype circulation and three amino acids substitutions (E1-K211T, E1-M269V, E1-T288I) in positive patients. MAIN CONCLUSION The results indicate the bioequivalence of serum and plasma for CHIKV diagnosis, with urine being an important complement. ECSA genotype was circulating among patients in the period of the 2016 outbreak with K211T, M269V and T288I substitution.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762022000101103&tlng=enCHIKVoncological patientsviral loadECSA |
| spellingShingle | Débora Familiar-Macedo Bianca Ervatti Gama Vanessa Erichsen Emmel Gabriela Vera-Lozada Eliana Abdelhay Ianick Souto Martins Rocio Hassan Molecular aspects of Chikungunya virus infections in cancer patients Memorias do Instituto Oswaldo Cruz CHIKV oncological patients viral load ECSA |
| title | Molecular aspects of Chikungunya virus infections in cancer patients |
| title_full | Molecular aspects of Chikungunya virus infections in cancer patients |
| title_fullStr | Molecular aspects of Chikungunya virus infections in cancer patients |
| title_full_unstemmed | Molecular aspects of Chikungunya virus infections in cancer patients |
| title_short | Molecular aspects of Chikungunya virus infections in cancer patients |
| title_sort | molecular aspects of chikungunya virus infections in cancer patients |
| topic | CHIKV oncological patients viral load ECSA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762022000101103&tlng=en |
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