Host and Nonhost Resistance in Medicago–Colletotrichum Interactions
Medicago truncatula lines resistant (A17) or susceptible (F83005.5) to the alfalfa pathogen Colletotrichum trifolii were used to compare defense reactions induced upon inoculation with C. trifolii or with the nonadapted pathogens C. lindemuthianum and C. higginsianum. Nonadapted Colletotrichum spp....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2010-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-9-1107 |
| _version_ | 1819045940988739584 |
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| author | Valérie Jaulneau Marc Cazaux Joanne Wong Sak Hoi Sylvie Fournier Marie-Thérèse Esquerré-Tugayé Christophe Jacquet Bernard Dumas |
| author_facet | Valérie Jaulneau Marc Cazaux Joanne Wong Sak Hoi Sylvie Fournier Marie-Thérèse Esquerré-Tugayé Christophe Jacquet Bernard Dumas |
| author_sort | Valérie Jaulneau |
| collection | DOAJ |
| description | Medicago truncatula lines resistant (A17) or susceptible (F83005.5) to the alfalfa pathogen Colletotrichum trifolii were used to compare defense reactions induced upon inoculation with C. trifolii or with the nonadapted pathogens C. lindemuthianum and C. higginsianum. Nonadapted Colletotrichum spp. induced a hypersensitive response (HR)-like reaction similar to the one induced during the host-incompatible interaction. Molecular analyses indicated an induction of PR10 and chalcone synthase genes in host and nonhost interactions but delayed responses were observed in the F83005.5 line. The clste12 penetration-deficient C. lindemuthianum mutant induced an HR and defense gene expression, showing that perception of nonadapted strains occurs before penetration of epidermal cells. Cytological and transcriptomic analyses performed upon inoculation of near-isogenic M. truncatula lines, differing only at the C. trifolii resistance locus, Ct1, with the nonadapted Colletotrichum strain, showed that nonhost responses are similar in the two lines. These included a localized oxidative burst, accumulation of fluorescent compounds, and transient expression of a small number of genes. Host interactions were characterized by a group of defense and signaling-related genes induced at 3 days postinoculation, associated with an accumulation of salicylic acid. Together, these results show that M. truncatula displays a rapid and transient response to nonadapted Colletotrichum strains and that this response is not linked to the C. trifolii resistance locus. |
| first_indexed | 2024-12-21T10:36:34Z |
| format | Article |
| id | doaj.art-fa951a16ae9d44039d4860c84a7bd506 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-21T10:36:34Z |
| publishDate | 2010-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-fa951a16ae9d44039d4860c84a7bd5062022-12-21T19:07:04ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062010-09-012391107111710.1094/MPMI-23-9-1107Host and Nonhost Resistance in Medicago–Colletotrichum InteractionsValérie JaulneauMarc CazauxJoanne Wong Sak HoiSylvie FournierMarie-Thérèse Esquerré-TugayéChristophe JacquetBernard DumasMedicago truncatula lines resistant (A17) or susceptible (F83005.5) to the alfalfa pathogen Colletotrichum trifolii were used to compare defense reactions induced upon inoculation with C. trifolii or with the nonadapted pathogens C. lindemuthianum and C. higginsianum. Nonadapted Colletotrichum spp. induced a hypersensitive response (HR)-like reaction similar to the one induced during the host-incompatible interaction. Molecular analyses indicated an induction of PR10 and chalcone synthase genes in host and nonhost interactions but delayed responses were observed in the F83005.5 line. The clste12 penetration-deficient C. lindemuthianum mutant induced an HR and defense gene expression, showing that perception of nonadapted strains occurs before penetration of epidermal cells. Cytological and transcriptomic analyses performed upon inoculation of near-isogenic M. truncatula lines, differing only at the C. trifolii resistance locus, Ct1, with the nonadapted Colletotrichum strain, showed that nonhost responses are similar in the two lines. These included a localized oxidative burst, accumulation of fluorescent compounds, and transient expression of a small number of genes. Host interactions were characterized by a group of defense and signaling-related genes induced at 3 days postinoculation, associated with an accumulation of salicylic acid. Together, these results show that M. truncatula displays a rapid and transient response to nonadapted Colletotrichum strains and that this response is not linked to the C. trifolii resistance locus.https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-9-1107 |
| spellingShingle | Valérie Jaulneau Marc Cazaux Joanne Wong Sak Hoi Sylvie Fournier Marie-Thérèse Esquerré-Tugayé Christophe Jacquet Bernard Dumas Host and Nonhost Resistance in Medicago–Colletotrichum Interactions Molecular Plant-Microbe Interactions |
| title | Host and Nonhost Resistance in Medicago–Colletotrichum Interactions |
| title_full | Host and Nonhost Resistance in Medicago–Colletotrichum Interactions |
| title_fullStr | Host and Nonhost Resistance in Medicago–Colletotrichum Interactions |
| title_full_unstemmed | Host and Nonhost Resistance in Medicago–Colletotrichum Interactions |
| title_short | Host and Nonhost Resistance in Medicago–Colletotrichum Interactions |
| title_sort | host and nonhost resistance in medicago colletotrichum interactions |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-9-1107 |
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