Feedstock/pretreatment screening for bioconversion of sugar and lignin streams via deacetylated disc-refining
Abstract Recent publications have shown the benefits of deacetylation disc-refining (DDR) as a pretreatment process to deconstruct biomass into sugars and lignin residues. Major advantages of DDR pretreatment over steam and dilute acid pretreatment are the removal of acetyl and lignin during deacety...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Online Access: | https://doi.org/10.1186/s13068-024-02492-7 |
| _version_ | 1827572213849522176 |
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| author | Darren J. Peterson Changyub Paek Ling Tao Ryan Davis Xiaowen Chen Roman Brunecky Matthew Fowler Richard Elander |
| author_facet | Darren J. Peterson Changyub Paek Ling Tao Ryan Davis Xiaowen Chen Roman Brunecky Matthew Fowler Richard Elander |
| author_sort | Darren J. Peterson |
| collection | DOAJ |
| description | Abstract Recent publications have shown the benefits of deacetylation disc-refining (DDR) as a pretreatment process to deconstruct biomass into sugars and lignin residues. Major advantages of DDR pretreatment over steam and dilute acid pretreatment are the removal of acetyl and lignin during deacetylation. DDR does not generate hydroxymethylfurfural (HMF) and furfural which are commonly produced from steam and dilute acid pretreatments. Acetate, lignin, HMF, and furfural are known inhibitors during enzymatic hydrolysis and fermentation. Another advantage of deacetylation is the production of lignin-rich black liquor, which can be upgraded to other bioproducts. Furthermore, due to the lack of sugar degradation during deacetylation, DDR has significantly less sugar loss than other pretreatment methods. Previous studies for DDR have primarily focused on corn stover, but lacked the investigative studies of other feedstocks. This study was designed to screen various DDR process conditions at pilot scale using three different feedstocks, including corn stover, poplar, and switchgrass. The impact of the pretreatment conditions was evaluated by testing hydrolysates for bioconversion to 2,3-butanediol. Pretreatment of biomass by DDR showed high-conversion-yields and 2,3-BDO fermentation production yields. Techno-economic analysis (TEA) of the pretreatment for biomass to sugar was also developed based on NREL’s Aspen Model. This study shows that the cellulose and hemicellulose in poplar was more recalcitrant than herbaceous feedstocks which ultimately drove up the sugar cost. Switchgrass was also more recalcitrant than corn stover but less than poplar. |
| first_indexed | 2024-04-24T12:42:08Z |
| format | Article |
| id | doaj.art-fa954af7e31b45188c9686dba0210cbd |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-24T12:42:08Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-fa954af7e31b45188c9686dba0210cbd2024-04-07T11:11:33ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542024-04-0117112110.1186/s13068-024-02492-7Feedstock/pretreatment screening for bioconversion of sugar and lignin streams via deacetylated disc-refiningDarren J. Peterson0Changyub Paek1Ling Tao2Ryan Davis3Xiaowen Chen4Roman Brunecky5Matthew Fowler6Richard Elander7National Renewable Energy LaboratoryExxonMobil Technology and Engineering CompanyNational Renewable Energy LaboratoryNational Renewable Energy LaboratoryNational Renewable Energy LaboratoryNational Renewable Energy LaboratoryNational Renewable Energy LaboratoryNational Renewable Energy LaboratoryAbstract Recent publications have shown the benefits of deacetylation disc-refining (DDR) as a pretreatment process to deconstruct biomass into sugars and lignin residues. Major advantages of DDR pretreatment over steam and dilute acid pretreatment are the removal of acetyl and lignin during deacetylation. DDR does not generate hydroxymethylfurfural (HMF) and furfural which are commonly produced from steam and dilute acid pretreatments. Acetate, lignin, HMF, and furfural are known inhibitors during enzymatic hydrolysis and fermentation. Another advantage of deacetylation is the production of lignin-rich black liquor, which can be upgraded to other bioproducts. Furthermore, due to the lack of sugar degradation during deacetylation, DDR has significantly less sugar loss than other pretreatment methods. Previous studies for DDR have primarily focused on corn stover, but lacked the investigative studies of other feedstocks. This study was designed to screen various DDR process conditions at pilot scale using three different feedstocks, including corn stover, poplar, and switchgrass. The impact of the pretreatment conditions was evaluated by testing hydrolysates for bioconversion to 2,3-butanediol. Pretreatment of biomass by DDR showed high-conversion-yields and 2,3-BDO fermentation production yields. Techno-economic analysis (TEA) of the pretreatment for biomass to sugar was also developed based on NREL’s Aspen Model. This study shows that the cellulose and hemicellulose in poplar was more recalcitrant than herbaceous feedstocks which ultimately drove up the sugar cost. Switchgrass was also more recalcitrant than corn stover but less than poplar.https://doi.org/10.1186/s13068-024-02492-7 |
| spellingShingle | Darren J. Peterson Changyub Paek Ling Tao Ryan Davis Xiaowen Chen Roman Brunecky Matthew Fowler Richard Elander Feedstock/pretreatment screening for bioconversion of sugar and lignin streams via deacetylated disc-refining Biotechnology for Biofuels and Bioproducts |
| title | Feedstock/pretreatment screening for bioconversion of sugar and lignin streams via deacetylated disc-refining |
| title_full | Feedstock/pretreatment screening for bioconversion of sugar and lignin streams via deacetylated disc-refining |
| title_fullStr | Feedstock/pretreatment screening for bioconversion of sugar and lignin streams via deacetylated disc-refining |
| title_full_unstemmed | Feedstock/pretreatment screening for bioconversion of sugar and lignin streams via deacetylated disc-refining |
| title_short | Feedstock/pretreatment screening for bioconversion of sugar and lignin streams via deacetylated disc-refining |
| title_sort | feedstock pretreatment screening for bioconversion of sugar and lignin streams via deacetylated disc refining |
| url | https://doi.org/10.1186/s13068-024-02492-7 |
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