Deep sequencing identification of novel glucocorticoid-responsive miRNAs in apoptotic primary lymphocytes.
Apoptosis of lymphocytes governs the response of the immune system to environmental stress and toxic insult. Signaling through the ubiquitously expressed glucocorticoid receptor, stress-induced glucocorticoid hormones induce apoptosis via mechanisms requiring altered gene expression. Several reports...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3824063?pdf=render |
| _version_ | 1811331187038224384 |
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| author | Lindsay K Smith Arpit Tandon Ruchir R Shah Deepak Mav Alyson B Scoltock John A Cidlowski |
| author_facet | Lindsay K Smith Arpit Tandon Ruchir R Shah Deepak Mav Alyson B Scoltock John A Cidlowski |
| author_sort | Lindsay K Smith |
| collection | DOAJ |
| description | Apoptosis of lymphocytes governs the response of the immune system to environmental stress and toxic insult. Signaling through the ubiquitously expressed glucocorticoid receptor, stress-induced glucocorticoid hormones induce apoptosis via mechanisms requiring altered gene expression. Several reports have detailed the changes in gene expression mediating glucocorticoid-induced apoptosis of lymphocytes. However, few studies have examined the role of non-coding miRNAs in this essential physiological process. Previously, using hybridization-based gene expression analysis and deep sequencing of small RNAs, we described the prevalent post-transcriptional repression of annotated miRNAs during glucocorticoid-induced apoptosis of lymphocytes. Here, we describe the development of a customized bioinformatics pipeline that facilitates the deep sequencing-mediated discovery of novel glucocorticoid-responsive miRNAs in apoptotic primary lymphocytes. This analysis identifies the potential presence of over 200 novel glucocorticoid-responsive miRNAs. We have validated the expression of two novel glucocorticoid-responsive miRNAs using small RNA-specific qPCR. Furthermore, through the use of Ingenuity Pathways Analysis (IPA) we determined that the putative targets of these novel validated miRNAs are predicted to regulate cell death processes. These findings identify two and predict the presence of additional novel glucocorticoid-responsive miRNAs in the rat transcriptome, suggesting a potential role for both annotated and novel miRNAs in glucocorticoid-induced apoptosis of lymphocytes. |
| first_indexed | 2024-04-13T16:15:22Z |
| format | Article |
| id | doaj.art-fa9a7755c02b4a2abce104936014526b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T16:15:22Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fa9a7755c02b4a2abce104936014526b2022-12-22T02:40:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7831610.1371/journal.pone.0078316Deep sequencing identification of novel glucocorticoid-responsive miRNAs in apoptotic primary lymphocytes.Lindsay K SmithArpit TandonRuchir R ShahDeepak MavAlyson B ScoltockJohn A CidlowskiApoptosis of lymphocytes governs the response of the immune system to environmental stress and toxic insult. Signaling through the ubiquitously expressed glucocorticoid receptor, stress-induced glucocorticoid hormones induce apoptosis via mechanisms requiring altered gene expression. Several reports have detailed the changes in gene expression mediating glucocorticoid-induced apoptosis of lymphocytes. However, few studies have examined the role of non-coding miRNAs in this essential physiological process. Previously, using hybridization-based gene expression analysis and deep sequencing of small RNAs, we described the prevalent post-transcriptional repression of annotated miRNAs during glucocorticoid-induced apoptosis of lymphocytes. Here, we describe the development of a customized bioinformatics pipeline that facilitates the deep sequencing-mediated discovery of novel glucocorticoid-responsive miRNAs in apoptotic primary lymphocytes. This analysis identifies the potential presence of over 200 novel glucocorticoid-responsive miRNAs. We have validated the expression of two novel glucocorticoid-responsive miRNAs using small RNA-specific qPCR. Furthermore, through the use of Ingenuity Pathways Analysis (IPA) we determined that the putative targets of these novel validated miRNAs are predicted to regulate cell death processes. These findings identify two and predict the presence of additional novel glucocorticoid-responsive miRNAs in the rat transcriptome, suggesting a potential role for both annotated and novel miRNAs in glucocorticoid-induced apoptosis of lymphocytes.http://europepmc.org/articles/PMC3824063?pdf=render |
| spellingShingle | Lindsay K Smith Arpit Tandon Ruchir R Shah Deepak Mav Alyson B Scoltock John A Cidlowski Deep sequencing identification of novel glucocorticoid-responsive miRNAs in apoptotic primary lymphocytes. PLoS ONE |
| title | Deep sequencing identification of novel glucocorticoid-responsive miRNAs in apoptotic primary lymphocytes. |
| title_full | Deep sequencing identification of novel glucocorticoid-responsive miRNAs in apoptotic primary lymphocytes. |
| title_fullStr | Deep sequencing identification of novel glucocorticoid-responsive miRNAs in apoptotic primary lymphocytes. |
| title_full_unstemmed | Deep sequencing identification of novel glucocorticoid-responsive miRNAs in apoptotic primary lymphocytes. |
| title_short | Deep sequencing identification of novel glucocorticoid-responsive miRNAs in apoptotic primary lymphocytes. |
| title_sort | deep sequencing identification of novel glucocorticoid responsive mirnas in apoptotic primary lymphocytes |
| url | http://europepmc.org/articles/PMC3824063?pdf=render |
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