APPLICATION EFFICIENCY OF BOVINE SERUM ALBUMIN FOR RECOVERY OF SEMINIFEROUS TUBULES OF TESTES AFTER CRYOPRESERVATION
An optimal approach to the recovery of testicular tissue fragments after cryopreservation is critical for their further use in order to successful fertility restoration. Aim. The purpose of this study was to investigate the effect of bovine serum albumin (BSA) addition to the rehabilitation mediu...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2021-04-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/2_2021/volkova_2_2021.pdf |
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| author | N. O Volkova M. S. Yukhta L. V. Sokil L. G. Chernyschenko L. V. Stepanyuk A. M. Goltsev |
| author_facet | N. O Volkova M. S. Yukhta L. V. Sokil L. G. Chernyschenko L. V. Stepanyuk A. M. Goltsev |
| author_sort | N. O Volkova |
| collection | DOAJ |
| description | An optimal approach to the recovery of testicular tissue fragments after cryopreservation is critical for their further use in order to successful fertility restoration.
Aim. The purpose of this study was to investigate the effect of bovine serum albumin (BSA) addition to the rehabilitation medium on the morphofunctional characteristics of fragments of seminiferous tubules of testes (FSTT) of immature rats after cryopreservation.
Methods. The object of the study was cryopreserved by slow cooling and vitrified FSTT. Warmed samples were incubated for 30 min in Leibovitz medium supplemented with BSA at concentrations of 0, 2, 5 or 10%. After that, morphological characteristics, the activity of the metabolic and antioxidant systems were evaluated.
Results. It was found that in the samples cryopreserved by slow cooling, the use of 5% BSA contributed to the increase in the safety of spermatogenic epithelium cells, in the levels of metabolic and antioxidant activities. In case of vitrified FSTT samples, it was observed that the addition of 5% BSA to the medium promoted the repair of minor tissue damage and increased metabolic activity level, but did not affect the state of the antioxidant defense system.
Conclusions. The obtained data can be used to develop an effective rehabilitation medium for cryopreserved fragments of the seminiferous tubules of testes using BSA. |
| first_indexed | 2024-03-12T06:23:31Z |
| format | Article |
| id | doaj.art-fa9be74d9eb6483d84a56d9ee50afe77 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T06:23:31Z |
| publishDate | 2021-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-fa9be74d9eb6483d84a56d9ee50afe772023-09-03T02:07:53ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2021-04-01142283610.15407/biotech14.02.028APPLICATION EFFICIENCY OF BOVINE SERUM ALBUMIN FOR RECOVERY OF SEMINIFEROUS TUBULES OF TESTES AFTER CRYOPRESERVATIONN. O Volkova0M. S. Yukhta1 L. V. Sokil2L. G. Chernyschenko3 L. V. Stepanyuk4 A. M. Goltsev5Institute for Problems of Cryobiology and Cryomedicineof the National Academy of Sciences of Ukraine, KharkivInstitute for Problems of Cryobiology and Cryomedicineof the National Academy of Sciences of Ukraine, KharkivInstitute for Problems of Cryobiology and Cryomedicineof the National Academy of Sciences of Ukraine, KharkivInstitute for Problems of Cryobiology and Cryomedicineof the National Academy of Sciences of Ukraine, KharkivInstitute for Problems of Cryobiology and Cryomedicineof the National Academy of Sciences of Ukraine, KharkivInstitute for Problems of Cryobiology and Cryomedicineof the National Academy of Sciences of Ukraine, KharkivAn optimal approach to the recovery of testicular tissue fragments after cryopreservation is critical for their further use in order to successful fertility restoration. Aim. The purpose of this study was to investigate the effect of bovine serum albumin (BSA) addition to the rehabilitation medium on the morphofunctional characteristics of fragments of seminiferous tubules of testes (FSTT) of immature rats after cryopreservation. Methods. The object of the study was cryopreserved by slow cooling and vitrified FSTT. Warmed samples were incubated for 30 min in Leibovitz medium supplemented with BSA at concentrations of 0, 2, 5 or 10%. After that, morphological characteristics, the activity of the metabolic and antioxidant systems were evaluated. Results. It was found that in the samples cryopreserved by slow cooling, the use of 5% BSA contributed to the increase in the safety of spermatogenic epithelium cells, in the levels of metabolic and antioxidant activities. In case of vitrified FSTT samples, it was observed that the addition of 5% BSA to the medium promoted the repair of minor tissue damage and increased metabolic activity level, but did not affect the state of the antioxidant defense system. Conclusions. The obtained data can be used to develop an effective rehabilitation medium for cryopreserved fragments of the seminiferous tubules of testes using BSA.http://biotechnology.kiev.ua/images/storage/2_2021/volkova_2_2021.pdfbovine serum albuminrehabilitationcryopreservationfragments of the seminiferous tubules of testes. |
| spellingShingle | N. O Volkova M. S. Yukhta L. V. Sokil L. G. Chernyschenko L. V. Stepanyuk A. M. Goltsev APPLICATION EFFICIENCY OF BOVINE SERUM ALBUMIN FOR RECOVERY OF SEMINIFEROUS TUBULES OF TESTES AFTER CRYOPRESERVATION Biotechnologia Acta bovine serum albumin rehabilitation cryopreservation fragments of the seminiferous tubules of testes. |
| title | APPLICATION EFFICIENCY OF BOVINE SERUM ALBUMIN FOR RECOVERY OF SEMINIFEROUS TUBULES OF TESTES AFTER CRYOPRESERVATION |
| title_full | APPLICATION EFFICIENCY OF BOVINE SERUM ALBUMIN FOR RECOVERY OF SEMINIFEROUS TUBULES OF TESTES AFTER CRYOPRESERVATION |
| title_fullStr | APPLICATION EFFICIENCY OF BOVINE SERUM ALBUMIN FOR RECOVERY OF SEMINIFEROUS TUBULES OF TESTES AFTER CRYOPRESERVATION |
| title_full_unstemmed | APPLICATION EFFICIENCY OF BOVINE SERUM ALBUMIN FOR RECOVERY OF SEMINIFEROUS TUBULES OF TESTES AFTER CRYOPRESERVATION |
| title_short | APPLICATION EFFICIENCY OF BOVINE SERUM ALBUMIN FOR RECOVERY OF SEMINIFEROUS TUBULES OF TESTES AFTER CRYOPRESERVATION |
| title_sort | application efficiency of bovine serum albumin for recovery of seminiferous tubules of testes after cryopreservation |
| topic | bovine serum albumin rehabilitation cryopreservation fragments of the seminiferous tubules of testes. |
| url | http://biotechnology.kiev.ua/images/storage/2_2021/volkova_2_2021.pdf |
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