Glycine Effect on the Expression Profile of Orphan Receptors GPR21, GPR26, GPR39, GPR82 and GPR6 in a Model of Inflammation in 3T3-L1 Cells
Background: Chronic or low-grade inflammation is a process where various immune cells are recruited from the periphery into adipose tissue. This event gives rise to localised inflammation, in addition to having a close interaction with cardiometabolic pathologies where the mediation of orphan recept...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/2075-1729/12/11/1687 |
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| author | Rocío Alejandra Gutiérrez-Rojas Karla Aidee Aguayo-Cerón Cruz Vargas-De-León Sandra Edith Cabrera-Becerra Julio Cesar Almanza-Pérez Fengyang Huang Santiago Villafaña Rodrigo Romero-Nava |
| author_facet | Rocío Alejandra Gutiérrez-Rojas Karla Aidee Aguayo-Cerón Cruz Vargas-De-León Sandra Edith Cabrera-Becerra Julio Cesar Almanza-Pérez Fengyang Huang Santiago Villafaña Rodrigo Romero-Nava |
| author_sort | Rocío Alejandra Gutiérrez-Rojas |
| collection | DOAJ |
| description | Background: Chronic or low-grade inflammation is a process where various immune cells are recruited from the periphery into adipose tissue. This event gives rise to localised inflammation, in addition to having a close interaction with cardiometabolic pathologies where the mediation of orphan receptors is observed. The aim of this study was to analyse the participation of the orphan receptors GPR21, GPR39, GPR82 and GPR6 in a chronic inflammatory process in 3T3-L1 cells. The 3T3-L1 cells were stimulated with TNF-α (5 ng/mL) for 60 min as an inflammatory model. Gene expression was measured by RT-qPCR. Results: We showed that the inflammatory stimulus of TNF-α in adipocytes decreased the expression of the orphan receptors GPR21, GPR26, GPR39, GPR82 and GPR6, which are related to low-grade inflammation. Conclusions: Our results suggest that GPR21 and GPR82 are modulated by glycine, it shows a possible protective role in the presence of an inflammatory environment in adipocytes, and they could be a therapeutic target to decrease the inflammation in some diseases related to low-grade inflammation such as diabetes, obesity and metabolic syndrome. |
| first_indexed | 2024-03-09T18:56:18Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T18:56:18Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-fa9eafda23cf48b18b60bba09f6f20422023-11-24T05:29:18ZengMDPI AGLife2075-17292022-10-011211168710.3390/life12111687Glycine Effect on the Expression Profile of Orphan Receptors GPR21, GPR26, GPR39, GPR82 and GPR6 in a Model of Inflammation in 3T3-L1 CellsRocío Alejandra Gutiérrez-Rojas0Karla Aidee Aguayo-Cerón1Cruz Vargas-De-León2Sandra Edith Cabrera-Becerra3Julio Cesar Almanza-Pérez4Fengyang Huang5Santiago Villafaña6Rodrigo Romero-Nava7Escuela Nacional de Ciencias Biologicas del Instituto Politécnico Nacional, Ciudad de México 11340, MexicoLaboratorio de Señalización Intracelular, Sección de Estudios de Posgrado, Escuela Superior de Medicina del Instituto Politécnico Nacional, Ciudad de México 11340, MexicoLaboratorio de Señalización Intracelular, Sección de Estudios de Posgrado, Escuela Superior de Medicina del Instituto Politécnico Nacional, Ciudad de México 11340, MexicoLaboratorio de Señalización Intracelular, Sección de Estudios de Posgrado, Escuela Superior de Medicina del Instituto Politécnico Nacional, Ciudad de México 11340, MexicoLaboratorio de Farmacología, Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana-Iztapalapa (UAM-I), Ciudad de México 09340, MexicoLaboratorio de Investigación en Farmacología, Hospital Infantil de México Federico Gómez (HIMFG), Ciudad de México 06720, MexicoLaboratorio de Señalización Intracelular, Sección de Estudios de Posgrado, Escuela Superior de Medicina del Instituto Politécnico Nacional, Ciudad de México 11340, MexicoLaboratorio de Señalización Intracelular, Sección de Estudios de Posgrado, Escuela Superior de Medicina del Instituto Politécnico Nacional, Ciudad de México 11340, MexicoBackground: Chronic or low-grade inflammation is a process where various immune cells are recruited from the periphery into adipose tissue. This event gives rise to localised inflammation, in addition to having a close interaction with cardiometabolic pathologies where the mediation of orphan receptors is observed. The aim of this study was to analyse the participation of the orphan receptors GPR21, GPR39, GPR82 and GPR6 in a chronic inflammatory process in 3T3-L1 cells. The 3T3-L1 cells were stimulated with TNF-α (5 ng/mL) for 60 min as an inflammatory model. Gene expression was measured by RT-qPCR. Results: We showed that the inflammatory stimulus of TNF-α in adipocytes decreased the expression of the orphan receptors GPR21, GPR26, GPR39, GPR82 and GPR6, which are related to low-grade inflammation. Conclusions: Our results suggest that GPR21 and GPR82 are modulated by glycine, it shows a possible protective role in the presence of an inflammatory environment in adipocytes, and they could be a therapeutic target to decrease the inflammation in some diseases related to low-grade inflammation such as diabetes, obesity and metabolic syndrome.https://www.mdpi.com/2075-1729/12/11/1687orphan receptorsglycineinflammationadipokines |
| spellingShingle | Rocío Alejandra Gutiérrez-Rojas Karla Aidee Aguayo-Cerón Cruz Vargas-De-León Sandra Edith Cabrera-Becerra Julio Cesar Almanza-Pérez Fengyang Huang Santiago Villafaña Rodrigo Romero-Nava Glycine Effect on the Expression Profile of Orphan Receptors GPR21, GPR26, GPR39, GPR82 and GPR6 in a Model of Inflammation in 3T3-L1 Cells Life orphan receptors glycine inflammation adipokines |
| title | Glycine Effect on the Expression Profile of Orphan Receptors GPR21, GPR26, GPR39, GPR82 and GPR6 in a Model of Inflammation in 3T3-L1 Cells |
| title_full | Glycine Effect on the Expression Profile of Orphan Receptors GPR21, GPR26, GPR39, GPR82 and GPR6 in a Model of Inflammation in 3T3-L1 Cells |
| title_fullStr | Glycine Effect on the Expression Profile of Orphan Receptors GPR21, GPR26, GPR39, GPR82 and GPR6 in a Model of Inflammation in 3T3-L1 Cells |
| title_full_unstemmed | Glycine Effect on the Expression Profile of Orphan Receptors GPR21, GPR26, GPR39, GPR82 and GPR6 in a Model of Inflammation in 3T3-L1 Cells |
| title_short | Glycine Effect on the Expression Profile of Orphan Receptors GPR21, GPR26, GPR39, GPR82 and GPR6 in a Model of Inflammation in 3T3-L1 Cells |
| title_sort | glycine effect on the expression profile of orphan receptors gpr21 gpr26 gpr39 gpr82 and gpr6 in a model of inflammation in 3t3 l1 cells |
| topic | orphan receptors glycine inflammation adipokines |
| url | https://www.mdpi.com/2075-1729/12/11/1687 |
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