Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps
While there have been huge advances in the field of biosensors during the last decade, their integration into a microfluidic environment avoiding external tubing and pumping is still neglected. Herein, we show a new microfluidic design that integrates multiple reservoirs for reagent storage and sing...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-09-01
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| Series: | Micromachines |
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| Online Access: | http://www.mdpi.com/2072-666X/7/9/153 |
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| author | Sascha Geidel Sergio Peransi Llopis Manuel Rodrigo Graciela de Diego-Castilla Antonio Sousa Jörg Nestler Thomas Otto Thomas Gessner Victor Parro |
| author_facet | Sascha Geidel Sergio Peransi Llopis Manuel Rodrigo Graciela de Diego-Castilla Antonio Sousa Jörg Nestler Thomas Otto Thomas Gessner Victor Parro |
| author_sort | Sascha Geidel |
| collection | DOAJ |
| description | While there have been huge advances in the field of biosensors during the last decade, their integration into a microfluidic environment avoiding external tubing and pumping is still neglected. Herein, we show a new microfluidic design that integrates multiple reservoirs for reagent storage and single-use electrochemical pumps for time-controlled delivery of the liquids. The cartridge has been tested and validated with a silicon nitride-based photonic biosensor incorporating multiple optical ring resonators as sensing elements and an immunoassay as a potential target application. Based on experimental results obtained with a demonstration model, subcomponents were designed and existing protocols were adapted. The newly-designed microfluidic cartridges and photonic sensors were separately characterized on a technical basis and performed well. Afterwards, the sensor was functionalized for a protein detection. The microfluidic cartridge was loaded with the necessary assay reagents. The integrated pumps were programmed to drive the single process steps of an immunoassay. The prototype worked selectively, but only with a low sensitivity. Further work must be carried out to optimize biofunctionalization of the optical ring resonators and to have a more suitable flow velocity progression to enhance the system’s reproducibility. |
| first_indexed | 2024-04-12T23:52:00Z |
| format | Article |
| id | doaj.art-faab0ab684614454b9a725e3e1713f8c |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-04-12T23:52:00Z |
| publishDate | 2016-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-faab0ab684614454b9a725e3e1713f8c2022-12-22T03:11:40ZengMDPI AGMicromachines2072-666X2016-09-017915310.3390/mi7090153mi7090153Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot MicropumpsSascha Geidel0Sergio Peransi Llopis1Manuel Rodrigo2Graciela de Diego-Castilla3Antonio Sousa4Jörg Nestler5Thomas Otto6Thomas Gessner7Victor Parro8Fraunhofer Institute for Electronic Nanosystems (ENAS), Technologie-Campus 3, 09126 Chemnitz, GermanyDAS Photonics S.L., Valencia 46022, SpainDAS Photonics S.L., Valencia 46022, SpainCentro de Astrobiología (INTA-CSIC), Torrejon de Ardoz 28850, SpainEvoleo Technologies Lda, 442565 Maia, PortugalBiFlow Systems GmbH, 09126 Chemnitz, GermanyFraunhofer Institute for Electronic Nanosystems (ENAS), Technologie-Campus 3, 09126 Chemnitz, GermanyFraunhofer Institute for Electronic Nanosystems (ENAS), Technologie-Campus 3, 09126 Chemnitz, GermanyCentro de Astrobiología (INTA-CSIC), Torrejon de Ardoz 28850, SpainWhile there have been huge advances in the field of biosensors during the last decade, their integration into a microfluidic environment avoiding external tubing and pumping is still neglected. Herein, we show a new microfluidic design that integrates multiple reservoirs for reagent storage and single-use electrochemical pumps for time-controlled delivery of the liquids. The cartridge has been tested and validated with a silicon nitride-based photonic biosensor incorporating multiple optical ring resonators as sensing elements and an immunoassay as a potential target application. Based on experimental results obtained with a demonstration model, subcomponents were designed and existing protocols were adapted. The newly-designed microfluidic cartridges and photonic sensors were separately characterized on a technical basis and performed well. Afterwards, the sensor was functionalized for a protein detection. The microfluidic cartridge was loaded with the necessary assay reagents. The integrated pumps were programmed to drive the single process steps of an immunoassay. The prototype worked selectively, but only with a low sensitivity. Further work must be carried out to optimize biofunctionalization of the optical ring resonators and to have a more suitable flow velocity progression to enhance the system’s reproducibility.http://www.mdpi.com/2072-666X/7/9/153microfluidicsbiosensoroptical ring resonatormicropumpslab-on-a-chip |
| spellingShingle | Sascha Geidel Sergio Peransi Llopis Manuel Rodrigo Graciela de Diego-Castilla Antonio Sousa Jörg Nestler Thomas Otto Thomas Gessner Victor Parro Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps Micromachines microfluidics biosensor optical ring resonator micropumps lab-on-a-chip |
| title | Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps |
| title_full | Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps |
| title_fullStr | Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps |
| title_full_unstemmed | Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps |
| title_short | Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps |
| title_sort | integration of an optical ring resonator biosensor into a self contained microfluidic cartridge with active single shot micropumps |
| topic | microfluidics biosensor optical ring resonator micropumps lab-on-a-chip |
| url | http://www.mdpi.com/2072-666X/7/9/153 |
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