Phenolic content, antioxidant activities and stimulatory roles of citrus fruits on some lactic acid bacteria
In this study, phenolic compounds and antioxidant activities in citrus fruits and their peels were determined, and their stimulatory roles on some lactic acid bacteria were investigated. Phenolic compounds in citrus fruits such as mandarin, lemon, orange and grapefruit were determined eithe...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
University of Belgrade, University of Novi Sad
2015-01-01
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Series: | Archives of Biological Sciences |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500108I.pdf |
Summary: | In this study, phenolic compounds and antioxidant activities in citrus fruits
and their peels were determined, and their stimulatory roles on some lactic
acid bacteria were investigated. Phenolic compounds in citrus fruits such as
mandarin, lemon, orange and grapefruit were determined either in the juices
or in the peel extracts. Total phenolic content was determined in a
spectrophotometer at 685 nm using the adapted Folin-Ciocalteu method. Total
flavonoid content was measured using LC/MS (liquid chromatography-mass
spectrometry). The effects of the fruit juices and peel extracts on the
selected lactic acid bacteria (Lactobacillus delbrueckii NRRL B5448, Lb.
casei NRRL B1922, Lb. acidophilus NRRL B4495) were investigated. The tested
lactic acid bacteria were significantly affected by chlorogenic acid,
hesperidin, naringin and caffeic acid compared to the control samples
(P≤0.05). Antioxidant properties of fruit samples were also measured using
the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The phenolics positively
affected the metabolism of bacteria, with the stimulatory effects of the
assayed samples being influenced by the phenolic profile. |
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ISSN: | 0354-4664 1821-4339 |