Manipulation of <i>CBTS1</i> Expression Alters Tobacco Resistance to <i>Spodoptera frugiperda</i> and <i>Phytophthora nicotianae</i>
Cembranoids produced by tobacco glandular trichomes have bioactivities in resistance to insect pests and pathogens. Cembratrien-ol synthase (CBTS) plays a key role in the biosynthesis of cembranoids and directly determines the cembranoid content in tobacco. This study examined the effect of changing...
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MDPI AG
2023-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/3/845 |
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| author | Jian Guan Zaifeng Du Tian Tian Wenjing Wang Fuzhu Ju Xiaoyang Lin Zhongfeng Zhang Yi Cao Hongbo Zhang |
| author_facet | Jian Guan Zaifeng Du Tian Tian Wenjing Wang Fuzhu Ju Xiaoyang Lin Zhongfeng Zhang Yi Cao Hongbo Zhang |
| author_sort | Jian Guan |
| collection | DOAJ |
| description | Cembranoids produced by tobacco glandular trichomes have bioactivities in resistance to insect pests and pathogens. Cembratrien-ol synthase (CBTS) plays a key role in the biosynthesis of cembranoids and directly determines the cembranoid content in tobacco. This study examined the effect of changing <i>CBTS1</i> expression on tobacco resistance to the insect pest <i>Spodoptera frugiperda</i> and oomycete pathogen <i>Phytophthora nicotianae</i>. The CDS sequence of <i>CBTS1</i> was cloned into gene overexpression and silencing vectors and introduced into tobacco (<i>Nicotiana tabacum</i> L. cv. TN90) to obtain <i>CBTS1</i>-overexpression plants (CBTS1-OE) and <i>CBTS1</i>-silenced plants (CBTS1-RI). Compared with control plants, the content of cembratrien-ol (CBT-ol) was increased 4.48 times in the CBTS1-OE plants but decreased by 68% in the CBTS1-RI plants, while that of cembratrien-diol (CBT-diol) was increased 3.17 times in the CBTS1-OE plants but decreased by 76% in the CBTS1-RI plants. The <i>S. frugiperda</i> resistance of transgenic tobacco plants was evaluated by in vitro toxicity test, and the results showed that the resistance of CBTS1-OE plants to <i>S. frugiperda</i> was significantly improved but that of CBTS1-RI plants was reduced. The <i>P. nicotianae</i> resistance of transgenic tobacco plants was assessed by the detached leaf assay, and the results showed that the resistance of CBTS1-OE plants to <i>P. nicotianae</i> was enhanced, while that of CBTS1-RI plants was attenuated. Further gene expression analysis showed that overexpression of <i>CBTS1</i> increased the expression of the pathogen-related gene <i>PR-1a</i>, while silencing of <i>CBTS1</i> decreased its expression. This study demonstrated that manipulating the expression of <i>CBTS1</i> could change the cembranoid content in tobacco plants and alter their resistance to both insect pests and oomycete pathogens. |
| first_indexed | 2024-03-11T07:03:05Z |
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| id | doaj.art-faae364762944e5f8f1f587baaac8750 |
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| spelling | doaj.art-faae364762944e5f8f1f587baaac87502023-11-17T09:07:03ZengMDPI AGAgronomy2073-43952023-03-0113384510.3390/agronomy13030845Manipulation of <i>CBTS1</i> Expression Alters Tobacco Resistance to <i>Spodoptera frugiperda</i> and <i>Phytophthora nicotianae</i>Jian Guan0Zaifeng Du1Tian Tian2Wenjing Wang3Fuzhu Ju4Xiaoyang Lin5Zhongfeng Zhang6Yi Cao7Hongbo Zhang8Key Laboratory of Synthetic Biology of Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory of Synthetic Biology of Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory of Synthetic Biology of Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory of Synthetic Biology of Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory of Synthetic Biology of Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory of Synthetic Biology of Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaKey Laboratory of Synthetic Biology of Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaAgronomy Research Center, Academy of Guizhou Tobacco Sciences, Guiyang 550081, ChinaKey Laboratory of Synthetic Biology of Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaCembranoids produced by tobacco glandular trichomes have bioactivities in resistance to insect pests and pathogens. Cembratrien-ol synthase (CBTS) plays a key role in the biosynthesis of cembranoids and directly determines the cembranoid content in tobacco. This study examined the effect of changing <i>CBTS1</i> expression on tobacco resistance to the insect pest <i>Spodoptera frugiperda</i> and oomycete pathogen <i>Phytophthora nicotianae</i>. The CDS sequence of <i>CBTS1</i> was cloned into gene overexpression and silencing vectors and introduced into tobacco (<i>Nicotiana tabacum</i> L. cv. TN90) to obtain <i>CBTS1</i>-overexpression plants (CBTS1-OE) and <i>CBTS1</i>-silenced plants (CBTS1-RI). Compared with control plants, the content of cembratrien-ol (CBT-ol) was increased 4.48 times in the CBTS1-OE plants but decreased by 68% in the CBTS1-RI plants, while that of cembratrien-diol (CBT-diol) was increased 3.17 times in the CBTS1-OE plants but decreased by 76% in the CBTS1-RI plants. The <i>S. frugiperda</i> resistance of transgenic tobacco plants was evaluated by in vitro toxicity test, and the results showed that the resistance of CBTS1-OE plants to <i>S. frugiperda</i> was significantly improved but that of CBTS1-RI plants was reduced. The <i>P. nicotianae</i> resistance of transgenic tobacco plants was assessed by the detached leaf assay, and the results showed that the resistance of CBTS1-OE plants to <i>P. nicotianae</i> was enhanced, while that of CBTS1-RI plants was attenuated. Further gene expression analysis showed that overexpression of <i>CBTS1</i> increased the expression of the pathogen-related gene <i>PR-1a</i>, while silencing of <i>CBTS1</i> decreased its expression. This study demonstrated that manipulating the expression of <i>CBTS1</i> could change the cembranoid content in tobacco plants and alter their resistance to both insect pests and oomycete pathogens.https://www.mdpi.com/2073-4395/13/3/845cembranoidcembratrien-ol synthase<i>Spodoptera frugiperda</i><i>Phytophthora nicotianae</i><i>PR</i> gene |
| spellingShingle | Jian Guan Zaifeng Du Tian Tian Wenjing Wang Fuzhu Ju Xiaoyang Lin Zhongfeng Zhang Yi Cao Hongbo Zhang Manipulation of <i>CBTS1</i> Expression Alters Tobacco Resistance to <i>Spodoptera frugiperda</i> and <i>Phytophthora nicotianae</i> Agronomy cembranoid cembratrien-ol synthase <i>Spodoptera frugiperda</i> <i>Phytophthora nicotianae</i> <i>PR</i> gene |
| title | Manipulation of <i>CBTS1</i> Expression Alters Tobacco Resistance to <i>Spodoptera frugiperda</i> and <i>Phytophthora nicotianae</i> |
| title_full | Manipulation of <i>CBTS1</i> Expression Alters Tobacco Resistance to <i>Spodoptera frugiperda</i> and <i>Phytophthora nicotianae</i> |
| title_fullStr | Manipulation of <i>CBTS1</i> Expression Alters Tobacco Resistance to <i>Spodoptera frugiperda</i> and <i>Phytophthora nicotianae</i> |
| title_full_unstemmed | Manipulation of <i>CBTS1</i> Expression Alters Tobacco Resistance to <i>Spodoptera frugiperda</i> and <i>Phytophthora nicotianae</i> |
| title_short | Manipulation of <i>CBTS1</i> Expression Alters Tobacco Resistance to <i>Spodoptera frugiperda</i> and <i>Phytophthora nicotianae</i> |
| title_sort | manipulation of i cbts1 i expression alters tobacco resistance to i spodoptera frugiperda i and i phytophthora nicotianae i |
| topic | cembranoid cembratrien-ol synthase <i>Spodoptera frugiperda</i> <i>Phytophthora nicotianae</i> <i>PR</i> gene |
| url | https://www.mdpi.com/2073-4395/13/3/845 |
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