Antioxidant Defense during Recovery of Resurrection Plant <i>Haberlea rhodopensis</i> from Drought- and Freezing-Induced Desiccation
In this study, the contribution of nonenzymatic (ascorbate, glutathione) and enzymatic antioxidants (superoxide dismutase, catalase, glutathione reductase, glutathione S-transferase) in the first hours of recovery of the resurrection plant <i>Haberlea rhodopensis</i> from drought- and fr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/2/175 |
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| author | Gergana Mihailova Ivanina Vasileva Liliana Gigova Emiliya Gesheva Lyudmila Simova-Stoilova Katya Georgieva |
| author_facet | Gergana Mihailova Ivanina Vasileva Liliana Gigova Emiliya Gesheva Lyudmila Simova-Stoilova Katya Georgieva |
| author_sort | Gergana Mihailova |
| collection | DOAJ |
| description | In this study, the contribution of nonenzymatic (ascorbate, glutathione) and enzymatic antioxidants (superoxide dismutase, catalase, glutathione reductase, glutathione S-transferase) in the first hours of recovery of the resurrection plant <i>Haberlea rhodopensis</i> from drought- and freezing-induced desiccation was assessed. The initial stage of recovery after desiccation is critical for plants, but less investigated. To better understand the alterations in the activity of antioxidant enzymes, their isoenzyme patterns were determined. Our results showed that ascorbate content remained high during the first 9 h of rehydration of desiccated plants and declined when the leaves′ water content significantly increased. The glutathione content remained high at the first hour of rehydration and then strongly decreased. The changes in ascorbate and glutathione content during recovery from drought- and freezing-induced desiccation showed great similarity. At the beginning of rehydration (1–5 h), the activities of antioxidant enzymes were significantly increased or remained as in dry plants. During 7–24 h of rehydration, certain differences in the enzymatic responses between the two plant groups were registered. The maintenance of a high antioxidant activity and upregulation of individual enzyme isoforms indicated their essential role in protecting plants from oxidative damage during the onset of recovery. |
| first_indexed | 2024-03-10T00:41:46Z |
| format | Article |
| id | doaj.art-fab66129f74a41f88c0216f5048e914b |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T00:41:46Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-fab66129f74a41f88c0216f5048e914b2023-11-23T15:07:04ZengMDPI AGPlants2223-77472022-01-0111217510.3390/plants11020175Antioxidant Defense during Recovery of Resurrection Plant <i>Haberlea rhodopensis</i> from Drought- and Freezing-Induced DesiccationGergana Mihailova0Ivanina Vasileva1Liliana Gigova2Emiliya Gesheva3Lyudmila Simova-Stoilova4Katya Georgieva5Laboratory of Photosynthesis–Activity and Regulation, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, BulgariaLaboratory of Experimental Algology, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 23, 1113 Sofia, BulgariaLaboratory of Experimental Algology, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 23, 1113 Sofia, BulgariaLaboratory of Plant-Soil Interactions, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, BulgariaLaboratory of Regulation of Gene Expression, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, BulgariaLaboratory of Photosynthesis–Activity and Regulation, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, BulgariaIn this study, the contribution of nonenzymatic (ascorbate, glutathione) and enzymatic antioxidants (superoxide dismutase, catalase, glutathione reductase, glutathione S-transferase) in the first hours of recovery of the resurrection plant <i>Haberlea rhodopensis</i> from drought- and freezing-induced desiccation was assessed. The initial stage of recovery after desiccation is critical for plants, but less investigated. To better understand the alterations in the activity of antioxidant enzymes, their isoenzyme patterns were determined. Our results showed that ascorbate content remained high during the first 9 h of rehydration of desiccated plants and declined when the leaves′ water content significantly increased. The glutathione content remained high at the first hour of rehydration and then strongly decreased. The changes in ascorbate and glutathione content during recovery from drought- and freezing-induced desiccation showed great similarity. At the beginning of rehydration (1–5 h), the activities of antioxidant enzymes were significantly increased or remained as in dry plants. During 7–24 h of rehydration, certain differences in the enzymatic responses between the two plant groups were registered. The maintenance of a high antioxidant activity and upregulation of individual enzyme isoforms indicated their essential role in protecting plants from oxidative damage during the onset of recovery.https://www.mdpi.com/2223-7747/11/2/175drought stresslow temperaturerehydrationascorbateglutathioneantioxidant enzymes |
| spellingShingle | Gergana Mihailova Ivanina Vasileva Liliana Gigova Emiliya Gesheva Lyudmila Simova-Stoilova Katya Georgieva Antioxidant Defense during Recovery of Resurrection Plant <i>Haberlea rhodopensis</i> from Drought- and Freezing-Induced Desiccation Plants drought stress low temperature rehydration ascorbate glutathione antioxidant enzymes |
| title | Antioxidant Defense during Recovery of Resurrection Plant <i>Haberlea rhodopensis</i> from Drought- and Freezing-Induced Desiccation |
| title_full | Antioxidant Defense during Recovery of Resurrection Plant <i>Haberlea rhodopensis</i> from Drought- and Freezing-Induced Desiccation |
| title_fullStr | Antioxidant Defense during Recovery of Resurrection Plant <i>Haberlea rhodopensis</i> from Drought- and Freezing-Induced Desiccation |
| title_full_unstemmed | Antioxidant Defense during Recovery of Resurrection Plant <i>Haberlea rhodopensis</i> from Drought- and Freezing-Induced Desiccation |
| title_short | Antioxidant Defense during Recovery of Resurrection Plant <i>Haberlea rhodopensis</i> from Drought- and Freezing-Induced Desiccation |
| title_sort | antioxidant defense during recovery of resurrection plant i haberlea rhodopensis i from drought and freezing induced desiccation |
| topic | drought stress low temperature rehydration ascorbate glutathione antioxidant enzymes |
| url | https://www.mdpi.com/2223-7747/11/2/175 |
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