Epiphitic microbiote and fungi contaminats from in vitro establishment of guajaba (Psidium guajava L.)
The studying of contaminats micobiote on guajaba (Psidium guajava L.) could help for creating schedule treatment of donator plants and its explants to eliminate or prevent the fungi contamination during guajaba micropropagation. The present work were focused on: qualitative evaluation of epiphytic m...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2002-04-01
|
| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/136 |
| _version_ | 1818921386948689920 |
|---|---|
| author | Mayra Acosta-Suárez Israel Caballero Yelenys Alvarado-Capó Michel Leiva-Mora |
| author_facet | Mayra Acosta-Suárez Israel Caballero Yelenys Alvarado-Capó Michel Leiva-Mora |
| author_sort | Mayra Acosta-Suárez |
| collection | DOAJ |
| description | The studying of contaminats micobiote on guajaba (Psidium guajava L.) could help for creating schedule treatment of donator plants and its explants to eliminate or prevent the fungi contamination during guajaba micropropagation. The present work were focused on: qualitative evaluation of epiphytic micobiote from explants of Enana Roja cubana EEA 18-40 plants (treated and not treated with fungicide), to isolate, characterize and identify the filamentous fungi from in vitro establishment of nodal segments. For the filamentous identification was used the wet chamber method and preparations were observed in optic microscopic. PDA Petri dishes were used to cultivate the filamentous fungi at 28ºC and dark during 7 to 14 days. The cultural and morphological characteristics were used to identifying each isolate. Nine filamentous fungi genera were identify on donator plants without any fungicide application, the principal genera’s were: Alternaria, Aspergillus, Cladosporium, Colletotrichum, Curvularia, Fusarium, Nigrospora, Penicillium and Trichoderma. The application of Mancozeb 80 PH (7.5g.l-1) and Benomyl 50 PH (4g.l-1) was not effective on epiphytic contaminants micobiote elimination. All these genera’s with the exception of Nigrospora were detected during the establishment of nodal segments. However the disinfection with hypochlorite at 3% for 10 minutes and HgCl2 solution at 0.05% and 0.1% could reduced the 50% of al contaminates genera’s.
Key words: micropropagation, microbiote contamination,filamentous fungi, surface dessinfectants. |
| first_indexed | 2024-12-20T01:36:50Z |
| format | Article |
| id | doaj.art-fab84634dccb4dc4ac7ae33d51636ab5 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-20T01:36:50Z |
| publishDate | 2002-04-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-fab84634dccb4dc4ac7ae33d51636ab52022-12-21T19:57:59ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472002-04-0122115Epiphitic microbiote and fungi contaminats from in vitro establishment of guajaba (Psidium guajava L.)Mayra Acosta-SuárezIsrael CaballeroYelenys Alvarado-CapóMichel Leiva-MoraThe studying of contaminats micobiote on guajaba (Psidium guajava L.) could help for creating schedule treatment of donator plants and its explants to eliminate or prevent the fungi contamination during guajaba micropropagation. The present work were focused on: qualitative evaluation of epiphytic micobiote from explants of Enana Roja cubana EEA 18-40 plants (treated and not treated with fungicide), to isolate, characterize and identify the filamentous fungi from in vitro establishment of nodal segments. For the filamentous identification was used the wet chamber method and preparations were observed in optic microscopic. PDA Petri dishes were used to cultivate the filamentous fungi at 28ºC and dark during 7 to 14 days. The cultural and morphological characteristics were used to identifying each isolate. Nine filamentous fungi genera were identify on donator plants without any fungicide application, the principal genera’s were: Alternaria, Aspergillus, Cladosporium, Colletotrichum, Curvularia, Fusarium, Nigrospora, Penicillium and Trichoderma. The application of Mancozeb 80 PH (7.5g.l-1) and Benomyl 50 PH (4g.l-1) was not effective on epiphytic contaminants micobiote elimination. All these genera’s with the exception of Nigrospora were detected during the establishment of nodal segments. However the disinfection with hypochlorite at 3% for 10 minutes and HgCl2 solution at 0.05% and 0.1% could reduced the 50% of al contaminates genera’s. Key words: micropropagation, microbiote contamination,filamentous fungi, surface dessinfectants.https://revista.ibp.co.cu/index.php/BV/article/view/136 |
| spellingShingle | Mayra Acosta-Suárez Israel Caballero Yelenys Alvarado-Capó Michel Leiva-Mora Epiphitic microbiote and fungi contaminats from in vitro establishment of guajaba (Psidium guajava L.) Biotecnología Vegetal |
| title | Epiphitic microbiote and fungi contaminats from in vitro establishment of guajaba (Psidium guajava L.) |
| title_full | Epiphitic microbiote and fungi contaminats from in vitro establishment of guajaba (Psidium guajava L.) |
| title_fullStr | Epiphitic microbiote and fungi contaminats from in vitro establishment of guajaba (Psidium guajava L.) |
| title_full_unstemmed | Epiphitic microbiote and fungi contaminats from in vitro establishment of guajaba (Psidium guajava L.) |
| title_short | Epiphitic microbiote and fungi contaminats from in vitro establishment of guajaba (Psidium guajava L.) |
| title_sort | epiphitic microbiote and fungi contaminats from in vitro establishment of guajaba psidium guajava l |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/136 |
| work_keys_str_mv | AT mayraacostasuarez epiphiticmicrobioteandfungicontaminatsfrominvitroestablishmentofguajabapsidiumguajaval AT israelcaballero epiphiticmicrobioteandfungicontaminatsfrominvitroestablishmentofguajabapsidiumguajaval AT yelenysalvaradocapo epiphiticmicrobioteandfungicontaminatsfrominvitroestablishmentofguajabapsidiumguajaval AT michelleivamora epiphiticmicrobioteandfungicontaminatsfrominvitroestablishmentofguajabapsidiumguajaval |