Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin
A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2010-03-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/10/4/2851/ |
| _version_ | 1828153752551424000 |
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| author | Fatma Ismail Samuel B. Adeloju |
| author_facet | Fatma Ismail Samuel B. Adeloju |
| author_sort | Fatma Ismail |
| collection | DOAJ |
| description | A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished with monomer solutions which contained 0.03 M tyramine, 37 U/mL penicillinase, 0.01 M KNO3,and 3 mM penicillin with an applied current density of 0.8 mA/cm2 and an electropolymerisation time of 40 seconds. The potentiometric biosensor gave a linear concentration range of 3–283 µM for penicillin and achieved a minimum detectable concentration of 0.3 µM. The biosensor was successfully utilized for the detection of Amoxycillin and gave an average percentage recovery of 102 ± 6%. Satisfactory recoveries of penicillin G were also achieved in milk samples with the potentiometric biosensor when concentrations are ≥20 ppm. |
| first_indexed | 2024-04-11T22:29:48Z |
| format | Article |
| id | doaj.art-fab8ff8d6c1342feb1b5f959c843fe82 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:29:48Z |
| publishDate | 2010-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-fab8ff8d6c1342feb1b5f959c843fe822022-12-22T03:59:31ZengMDPI AGSensors1424-82202010-03-011042851286810.3390/s100402851Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of PenicillinFatma IsmailSamuel B. AdelojuA sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished with monomer solutions which contained 0.03 M tyramine, 37 U/mL penicillinase, 0.01 M KNO3,and 3 mM penicillin with an applied current density of 0.8 mA/cm2 and an electropolymerisation time of 40 seconds. The potentiometric biosensor gave a linear concentration range of 3–283 µM for penicillin and achieved a minimum detectable concentration of 0.3 µM. The biosensor was successfully utilized for the detection of Amoxycillin and gave an average percentage recovery of 102 ± 6%. Satisfactory recoveries of penicillin G were also achieved in milk samples with the potentiometric biosensor when concentrations are ≥20 ppm.http://www.mdpi.com/1424-8220/10/4/2851/penicillinpenicillinasepolytyraminebiosensorpotentiometry |
| spellingShingle | Fatma Ismail Samuel B. Adeloju Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin Sensors penicillin penicillinase polytyramine biosensor potentiometry |
| title | Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin |
| title_full | Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin |
| title_fullStr | Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin |
| title_full_unstemmed | Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin |
| title_short | Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin |
| title_sort | galvanostatic entrapment of penicillinase into polytyramine films and its utilization for the potentiometric determination of penicillin |
| topic | penicillin penicillinase polytyramine biosensor potentiometry |
| url | http://www.mdpi.com/1424-8220/10/4/2851/ |
| work_keys_str_mv | AT fatmaismail galvanostaticentrapmentofpenicillinaseintopolytyraminefilmsanditsutilizationforthepotentiometricdeterminationofpenicillin AT samuelbadeloju galvanostaticentrapmentofpenicillinaseintopolytyraminefilmsanditsutilizationforthepotentiometricdeterminationofpenicillin |