Floridean Starch and Floridoside Metabolic Pathways of <i>Neoporphyra haitanensis</i> and Their Regulatory Mechanism under Continuous Darkness
Floridean starch and floridoside are the main storage carbohydrates of red algae. However, their complete metabolic pathways and the origin, function, and regulatory mechanism of their pathway genes have not been fully elucidated. In this study, we identified their metabolic pathway genes and analyz...
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MDPI AG
2021-11-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/12/664 |
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| author | Yahui Yu Xuli Jia Wenlei Wang Yuemei Jin Weizhi Liu Dongmei Wang Yunxiang Mao Chaotian Xie Tao Liu |
| author_facet | Yahui Yu Xuli Jia Wenlei Wang Yuemei Jin Weizhi Liu Dongmei Wang Yunxiang Mao Chaotian Xie Tao Liu |
| author_sort | Yahui Yu |
| collection | DOAJ |
| description | Floridean starch and floridoside are the main storage carbohydrates of red algae. However, their complete metabolic pathways and the origin, function, and regulatory mechanism of their pathway genes have not been fully elucidated. In this study, we identified their metabolic pathway genes and analyzed the changes in related gene expression and metabolite content in <i>Neoporphyra haitanensis</i> under continuous dark conditions. Our results showed that genes from different sources, including eukaryotic hosts, cyanobacteria, and bacteria, were combined to construct floridean starch and floridoside metabolic pathways in <i>N. haitanensis</i>. Moreover, compared with those in the control, under continuous dark conditions, floridean starch biosynthesis genes and some degradation genes were significantly upregulated with no significant change in floridean starch content, whereas floridoside degradation genes were significantly upregulated with a significant decrease in floridoside content. This implies that floridean starch content is maintained but floridoside is consumed in <i>N. haitanensis</i> under dark conditions. This study elucidates the “floridean starch–floridoside” metabolic network and its gene origins in <i>N. haitanensis</i> for the first time. |
| first_indexed | 2024-03-10T03:41:35Z |
| format | Article |
| id | doaj.art-fabb121f197e4ad7ac69ff90bb442e18 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T03:41:35Z |
| publishDate | 2021-11-01 |
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| series | Marine Drugs |
| spelling | doaj.art-fabb121f197e4ad7ac69ff90bb442e182023-11-23T09:17:55ZengMDPI AGMarine Drugs1660-33972021-11-01191266410.3390/md19120664Floridean Starch and Floridoside Metabolic Pathways of <i>Neoporphyra haitanensis</i> and Their Regulatory Mechanism under Continuous DarknessYahui Yu0Xuli Jia1Wenlei Wang2Yuemei Jin3Weizhi Liu4Dongmei Wang5Yunxiang Mao6Chaotian Xie7Tao Liu8College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaFisheries College, Jimei University, Xiamen 361021, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Utilization and Conservation for Tropical Marine Bioresources (Hainan Tropical Ocean University), Ministry of Education, Sanya 572022, ChinaFisheries College, Jimei University, Xiamen 361021, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaFloridean starch and floridoside are the main storage carbohydrates of red algae. However, their complete metabolic pathways and the origin, function, and regulatory mechanism of their pathway genes have not been fully elucidated. In this study, we identified their metabolic pathway genes and analyzed the changes in related gene expression and metabolite content in <i>Neoporphyra haitanensis</i> under continuous dark conditions. Our results showed that genes from different sources, including eukaryotic hosts, cyanobacteria, and bacteria, were combined to construct floridean starch and floridoside metabolic pathways in <i>N. haitanensis</i>. Moreover, compared with those in the control, under continuous dark conditions, floridean starch biosynthesis genes and some degradation genes were significantly upregulated with no significant change in floridean starch content, whereas floridoside degradation genes were significantly upregulated with a significant decrease in floridoside content. This implies that floridean starch content is maintained but floridoside is consumed in <i>N. haitanensis</i> under dark conditions. This study elucidates the “floridean starch–floridoside” metabolic network and its gene origins in <i>N. haitanensis</i> for the first time.https://www.mdpi.com/1660-3397/19/12/664darknessfloridean starchfloridosidemetabolic pathway<i>Neoporphyra haitanensis</i> |
| spellingShingle | Yahui Yu Xuli Jia Wenlei Wang Yuemei Jin Weizhi Liu Dongmei Wang Yunxiang Mao Chaotian Xie Tao Liu Floridean Starch and Floridoside Metabolic Pathways of <i>Neoporphyra haitanensis</i> and Their Regulatory Mechanism under Continuous Darkness Marine Drugs darkness floridean starch floridoside metabolic pathway <i>Neoporphyra haitanensis</i> |
| title | Floridean Starch and Floridoside Metabolic Pathways of <i>Neoporphyra haitanensis</i> and Their Regulatory Mechanism under Continuous Darkness |
| title_full | Floridean Starch and Floridoside Metabolic Pathways of <i>Neoporphyra haitanensis</i> and Their Regulatory Mechanism under Continuous Darkness |
| title_fullStr | Floridean Starch and Floridoside Metabolic Pathways of <i>Neoporphyra haitanensis</i> and Their Regulatory Mechanism under Continuous Darkness |
| title_full_unstemmed | Floridean Starch and Floridoside Metabolic Pathways of <i>Neoporphyra haitanensis</i> and Their Regulatory Mechanism under Continuous Darkness |
| title_short | Floridean Starch and Floridoside Metabolic Pathways of <i>Neoporphyra haitanensis</i> and Their Regulatory Mechanism under Continuous Darkness |
| title_sort | floridean starch and floridoside metabolic pathways of i neoporphyra haitanensis i and their regulatory mechanism under continuous darkness |
| topic | darkness floridean starch floridoside metabolic pathway <i>Neoporphyra haitanensis</i> |
| url | https://www.mdpi.com/1660-3397/19/12/664 |
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